Monitoring the efficacy of bioremediation

Heitzer, A.

doi:10.1016/0167-7799(93)90156-4

Cited by 78 publications

(33 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[9][10][11][12] As reported by Heitzer and Sayler, while pollutants and intermediates are important chemical monitoring parameters, only the formation of end products such as CO 2 provides direct proof of the complete destruction and mineralization of a pollutant. 13 A Hewlett-Packard 5890A II gas chromatograph was used to analyze the headspaces from hermetically stopper Pyrex filter flask containing soil and liquid samples. The carrier gas was ultra-high purity helium.…”

Section: Determinationmentioning

confidence: 99%

Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

Rizzo¹,

Cunha²,

Santos³

et al. 2008

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

A biorremediação é uma alternativa atraente para o tratamento de solos contaminados com hidrocarbonetos de petróleo. No entanto, a efetividade do processo pode ser limitada não só pelas características do contaminante, mas também pela sua biodisponibilidade. Este trabalho tem como meta o estabelecimento de procedimento preliminar para identificar o fator principal (recalcitrância ou biodisponibilidade) que interfere negativamente na biodegradação, resultando em baixas eficiên-cias. Foram realizados testes em microcosmos contendo solo aplicando as técnicas de bioestímulo e bioaumento. Experimentos semelhantes foram conduzidos em meio líquido contendo óleo cru e microorganismos extraídos do solo contaminado, nutrientes e inóculo microbiano (Rhodoturula glutinis e Nocardia nova). Após 30 dias, o maior nível de biodegradação (98.8%) foi obtido na condição com bioaumento em meio líquido, indicando que o problema principal de biodegradação de hidrocarbonetos de petróleo pode estar associado à baixa biodisponibilidade destes no solo e não apenas a recalcitrância intrínseca do poluente residual.Bioremediation is an attractive alternative to treat soils contaminated with petroleum hydrocarbons. However, the effectiveness of biodegradation process can be limited by both contaminant characteristics and its bioavailability in soil. This work aims at establishing a preliminary procedure to identify the main factor (hydrocarbon recalcitrance or its bioavailability) that impairs the biodegradation, possibly resulting in low remediation efficiencies. Tests in soil microcosms were carried out applying biostimulation and bioaugmentation techniques. Similar experiments were conducted using liquid medium containing crude oil and microorganisms, both of them extracted from a weathered contaminated soil sample, nutrients and microbial inoculum constituted of Rhodoturula glutinis and Nocardia nova. After 30 days, highest microcosms biodegradation levels (98.8%) were obtained with bioaugmentation in liquid medium, pointing out that the main problem of petroleum hydrocarbon utilization by microorganisms was associated to its availability and not only to the intrinsic recalcitrancy of remaining pollutants.

show abstract

Section: Determinationmentioning

confidence: 99%

Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

Rizzo¹,

Cunha²,

Santos³

et al. 2008

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Among these, biological remediation techniques constitute an attractive and cost-effective alternative (Heitzer & Sayler, 1993). However, the efficiency of the biological treatment of soils containing hydrophobic organic contaminants is highly dependent on contaminant bioavailability.…”

Section: Introductionmentioning

confidence: 99%

The effect of an anionic surfactant on the mobilization and biodegradation of PAHs in a creosote-contaminated soil

Deschênes

Lafrance

Villeneuve

et al. 1995

Hydrological Sciences Journal

View full text Add to dashboard Cite

The mobilization and biodégradation of 13 PAHs sorbed in a creosote-contaminated soil were assessed in the presence of sodium dodecyl sulphate (SDS). In a mobilization experiment, the soil was mixed with SDS solutions (0.005 to 1 % w/v) and the PAH concentrations in the aqueous phase were determined. In a biodégradation experiment, soil residual PAH concentrations were monitored during 45 weeks in the presence of 10, 100 and 500 mg kg" 1 SDS. The SDS was effective in mobilizing three-and four-ring PAHs. Increasing SDS concentration resulted in the mobilization of very low water-soluble five-and six-ring PAHs. The SDS (100 and~ 500 mg kg 4 ) significantly decreased the biodégradation of fluorene, phenanthrene and all of the four-ring PAHs. PAHs with more than four rings were not biodegraded. The surfactant [a 14 C]SDS was readily biodegraded. Even if SDS may be efficient in mobilizing PAHs in soil pore water or groundwater, it will not improve PAH biodégradation. Such a result must be considered when using anionic surfactants in the perspective of biological treatment of PAHs. Effet d'un agent tensio-actif anionique sur la mobilisation et la biodégradation de HAP présents dans un sol contaminé par la créosoteRésumé La mobilisation et la biodégradation de 13 HAP adsorbés sur un sol contaminé par la créosote ont été étudiées en présence de dodecyl sulphate de sodium (DSS). Pour la mobilisation, le sol a été mélangé avec des solutions de DSS (0.005 à 1 % p/v) et la concentration des HAP a été mesurée en phase aqueuse. Pour la biodégradation, les concentrations résiduelles de HAP dans le sol ont été mesurées durant 45 semaines en présence de 10,

show abstract

“…Biodegradation of the monoaromatic hydrocarbons, benzene, toluene, ethylbenzene, and the xylenes (collectively designated BTEX) has been extensively investigated as a basis for understanding the intrinsic biodegradation potential of these fuel hydrocarbons when they occur as groundwater contaminants (17,21,39,57,61). Toluene has been studied as a model compound representative of this group of aromatic hydrocarbons (14), and its biodegradation under aerobic conditions has been found to proceed by the six pathways shown in Fig.…”

mentioning

confidence: 99%

Genetic and Functional Analysis of the tbc Operons for Catabolism of Alkyl- and Chloroaromatic Compounds in Burkholderia sp. Strain JS150

Kahng¹,

Malinverni²,

Majko³

et al. 2001

Appl Environ Microbiol

View full text Add to dashboard Cite

Burkholderia sp. strain JS150 is able to metabolize a wide range of alkyl-and chloroaromatic hydrocarbons through multiple, apparently redundant catabolic pathways. Previous research has shown that strain JS150 is able to synthesize enzymes for multiple upper pathways as well as multiple lower pathways to accommodate variously substituted catechols that result from degradation of complex mixtures of monoaromatic compounds. We report here the genetic organization and functional characterization of a gene cluster, designated tbc (for toluene, benzene, and chlorobenzene utilization), which has been cloned as a 14.3-kb DNA fragment from strain JS150 into vector pRO1727. The cloned DNA fragment expressed in Pseudomonas aeruginosa PAO1c allowed the recombinant to grow on toluene or benzene and to transform chlorobenzene, trichloroethylene, phenol, and cresols. The tbc genes are organized into two divergently transcribed operons, tbc1 and tbc2, each comprised of six open reading frames. Similarity searches of databases revealed that the tbc1 and tbc2 genes showed significant homology to multicomponent cresol and phenol hydroxylases and to toluene and benzene monooxygenases, respectively. Deletion mutagenesis and product analysis were used to demonstrate that tbc2 plays a role in the initial catabolism of the unactivated alkyl-or chloroaromatic substrate and that the tbc1 gene products play a role in the catabolism of the first metabolite that results from transformation of the initial substrate. Phylogenetic analysis was used to compare individual components of these tbc monooxygenases with similar sequences in the databases. These results provide further evidence for the existence of multiple, functionally redundant alkyl-and chloroaromatic monooxygenases in strain JS150.Biodegradation of the monoaromatic hydrocarbons, benzene, toluene, ethylbenzene, and the xylenes (collectively designated BTEX) has been extensively investigated as a basis for understanding the intrinsic biodegradation potential of these fuel hydrocarbons when they occur as groundwater contaminants (17,21,39,57,61). Toluene has been studied as a model compound representative of this group of aromatic hydrocarbons (14), and its biodegradation under aerobic conditions has been found to proceed by the six pathways shown in Fig. 1. Implicit in much of the literature on biodegradation of toluene is the assumption that each toluene degrader elaborates a single pathway for toluene degradation. This is seen, for example, for Pseudomonas putida mt-2 (PaW1), which carries the TOL plasmid pWW0 (64); for P. putida F1 (13); for Burkholderia cepacia G4 (52); for Ralstonia pickettii PKO1 (25); or for Pseudomonas mendocina KR-1 (63). However, Burkholderia sp. strain JS150 seems to be an exception to this rule. Previous research conducted with this strain by Haigler and coworkers (15) has demonstrated that a broad-substrate-range toluene dioxygenase is at least partly responsible for the extended aromatic substrate range of JS150. Subsequently, the studies of Johnson...

show abstract

Monitoring the efficacy of bioremediation

Cited by 78 publications

References 21 publications

Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

Preliminary identification of the bioremediation limiting factors of a clay bearing soil contaminated with crude oil

The effect of an anionic surfactant on the mobilization and biodegradation of PAHs in a creosote-contaminated soil

Genetic and Functional Analysis of the tbc Operons for Catabolism of Alkyl- and Chloroaromatic Compounds in Burkholderia sp. Strain JS150

Contact Info

Product

Resources

About