Optical observations of Sco X-1 with exposure times around 0.5 s were performed with the 1.56 m telescope of the Shanghai Astronomical Observatory in 2008. With these observations, we studied the fluctuation of the optical flux on three short timescales (5, 10, 100 s). We found that the standard deviations of fluctuations on these three timescales differ from those of Gaussian distributions by 7 Sigma, 5 Sigma and 3 Sigma, respectively. The result suggests that the variations in the intensity on these short timescales differ from pure statistical fluctuations, which, we suggest, is due to the intrinsic variability in the optical emission.accretion disks, stars: individual (Scorpius X-1), optical: stars Sco X-1 is the brightest X-ray point source in the sky. It is a Z source, a subclass of low-mass X-ray binaries (LMXBs) [1], which traces out roughly a Z-shaped pattern in the X-ray color-color diagram. Its optical counterpart, V818 Sco, is a 13th magnitude star with excess UV emission. The optical emission is generally considered to originate mainly from the accretion disc rather than the donor star [2], and arises from the reprocessed X-ray emission of the accretion disk. The X-ray emission illuminates the accretion disk and heats the corona above the disk, and the optical emission arises from the hot plasma in the corona [3]. The X-ray flux variation may result in the variation of optical flux. There have been some other claims since the discovery of the narrow emission components arising from the donor star [4-6].Mcnamara et al. suggested a nearly linear relation between the B magnitude and the accretion rate on the normal and lower flaring branches [7]. Typically, a flux correlation between X-ray and optical occurs only when the object is brighter than the B magnitude around 12.7 [8-12], but there are some exceptions (e.g. refs. [13-15]). Several attempts have been made to study the erratic variability of Sco X-1 simultaneously in optical and X-ray, but there is no systematic understanding that has yet been achieved.Recent X-ray observations show X-ray variability on time scales as short as milliseconds (see a review in [16]). Some of the X-ray variability, such as the band-limited noise (BLN) on time scales from subseconds to tens of seconds, has been suggested to correspond to the variation of mass accretion rate in the disk [17]. The search for optical variability and the relation between optical and X-ray variability on short timescales would help reveal the origin of both the X-ray and optical variability. The study of optical variability on short timescales would open a new window for the study of the X-ray variability.In this paper, we show the optical behavior of Sco X-1 on short timescales of 5, 10 and 100 s and found evidence of rapid variability on these timescales in the optical emission of Sco X-1.