Monitoring the quality consistency of Fufang Danshen Pills using micellar electrokinetic chromatography fingerprint coupled with prediction of antioxidant activity and chemometrics

Ji, Zhengchao; Sun, Wanyang; Sun, Guoxiang; Zhang, Jin

doi:10.1002/jssc.201600334

Cited by 11 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values of IC 50 from DPPH, LPI and SRS assays were used as an excellent measurement indicator to compare the antioxidant activities of SSM and NSSM. When the IC 50 was lower, the antioxidant activity was better (Ji, Sun, Sun, & Zhang, ). The Student t ‐test was used to determine whether the values of IC 50 of SSM and NSSM differed from one another.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating

Fan

Wang

et al. 2018

Biomedical Chromatography

View full text Add to dashboard Cite

Salvia miltiorrhiza Bge. is a traditional Chinese medicine applied in the treatment of various diseases in clinical practice. In the course of its processing, S. miltiorrhiza Bge. is usually processed by sweating. This study employed 10-component contents determination coupled with high-performance liquid chromatography (HPLC) fingerprint and antioxidant activity to investigate the effect of sweating on S. miltiorrhiza Bge. so as to evaluate the quality of S. miltiorrhiza Bge. The HPLC method was performed using C and 0.05% phosphoric acid aqueous solution-acetonitrile with a gradient elution system. It was validated for linearity, precision, repeatability, stability and recovery. Similarity analysis, principal components analysis and antioxidant activity assays were used to compare sweated S. miltiorrhiza Bge. (SSM) and nonsweated S. miltiorrhiza Bge. (NSSM). SSM and NSSM showed good similarities in HPLC fingerprint (>0.9), but principal components analysis could classify the HPLC fingerprint and 10-component quantitation analysis. Meanwhile, the antioxidant activity of SSM was significantly higher than that of NSSM (p < 0.01). The results of this study indicated that sweating could alter the content of chemical constituents in S. miltiorrhiza Bge., and could also improve its antioxidant activity. In addition, the method not only affords a viable strategy for comparing SSM and NSSM and assessing the quality of S. miltiorrhiza Bge., but also provides a reference for other herbal medicine that suffers from sweating.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating

Fan

Wang

et al. 2018

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Therefore, it is necessary to establish a multi-criteria quality evaluation system. Chemical fingerprint analysis method was proposed and accepted for the identification of authenticity, differentiation of origin, and evaluation of quality of herbal medicines and related products, which could display the holistic chemical profile obtained by various analytical techniques [ 21 , 22 , 23 , 24 , 25 , 26 ]. Recently, chemometrics have attracted increasing attention with the development of data mining methodologies, which could simplify complex data and find hidden information.…”

Section: Introductionmentioning

confidence: 99%

Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. (Danshen) Based on HPLC Fingerprints and HPLC-MSn Coupled with Chemometrics

Liang

Chen

et al. 2017

Molecules

View full text Add to dashboard Cite

Danshen, the dried root of Salvia miltiorrhiza Bge., is a widely used commercially available herbal drug, and unstable quality of different samples is a current issue. This study focused on a comprehensive and systematic method combining fingerprints and chemical identification with chemometrics for discrimination and quality assessment of Danshen samples. Twenty-five samples were analyzed by HPLC-PAD and HPLC-MSn. Forty-nine components were identified and characteristic fragmentation regularities were summarized for further interpretation of bioactive components. Chemometric analysis was employed to differentiate samples and clarify the quality differences of Danshen including hierarchical cluster analysis, principal component analysis, and partial least squares discriminant analysis. Consistent results were that the samples were divided into three categories which reflected the difference in quality of Danshen samples. By analyzing the reasons for sample classification, it was revealed that the processing method had a more obvious impact on sample classification than the geographical origin, it induced the different content of bioactive compounds and finally lead to different qualities. Cryptotanshinone, trijuganone B, and 15,16-dihydrotanshinone I were screened out as markers to distinguish samples by different processing methods. The developed strategy could provide a reference for evaluation and discrimination of other traditional herbal medicines.

show abstract

“…First, sample and reference fingerprint vectors are respectively converted into

\vec{T_{s}} = false(\frac{x_{1}}{y_{1}}, \frac{x_{2}}{y_{2}}, \dots \frac{x_{n}}{y_{n}} false)

and

\vec{T_{r}} = false(\frac{y_{1}}{y_{1}}, \frac{y_{2}}{y_{2}}, \dots \frac{y_{n}}{y_{n}} false) = false(1, 1, 1 \dots 1 false)

, the cosine of the two vectors after the conversion is denoted as the qualitative ratio similarity (

S_{F}^{'}

), as calculated by Eq. . This conversion makes all the peaks in the same range.…”

Section: Theory and Principlesmentioning

confidence: 99%

Assessment of quality consistency in traditional Chinese medicine using multi‐wavelength fusion profiling by integrated quantitative fingerprint method: Niuhuang Jiedu pill as an example

Zhang

Sun

2018

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Under the wave of the revival of traditional Chinese medicine, there is a quite imperative duty to study an integrated and comprehensive method of fingerprint data processing and analysis on the quality consistency of traditional Chinese medicine. So, we proposed six parameters from two aspects (qualitative and quantitative), three levels (biased to strong peaks, biased to weak peaks, no obvious bias), to comprehensively evaluate the similarity of the two fingerprints. On this basis, another five parameters were proposed to evaluate the integrated effects (consistency, volatility, and similarity). This method was applied to 22 batches of Niuhuang Jiedu pill samples. Next, a practical and convenient multi‐wavelength fusion method was designed to provide more information, and the generated fusion profilings were used for subsequent evaluation. The characteristics of the parameters were confirmed by correlation analysis. The results of both hierarchical clustering analysis and principal component analysis for raw data and standardized data were consistent with integrated quantitative fingerprint method results. At the same time, this method gave a reasonable explanation for abnormal and dissimilar samples. This work illustrated that the proposed method was particularly suitable for similarity analysis of fingerprints and capable of ensuring the quality consistency in traditional Chinese medicine.

show abstract

Monitoring the quality consistency of Fufang Danshen Pills using micellar electrokinetic chromatography fingerprint coupled with prediction of antioxidant activity and chemometrics

Cited by 11 publications

References 36 publications

Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating

Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating

Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. (Danshen) Based on HPLC Fingerprints and HPLC-MSn Coupled with Chemometrics

Assessment of quality consistency in traditional Chinese medicine using multi‐wavelength fusion profiling by integrated quantitative fingerprint method: Niuhuang Jiedu pill as an example

Contact Info

Product

Resources

About