Monitoring toxicity of an azo dye methyl red and a heavy metal Cu, using plant and animal bioassays

Sharma, Shagun; Upreti, N.; Sharma, Kanta Prasad

doi:10.1080/02772240802010987

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…Sterile MilliQ water was used as a negative control while 0.03 mM Cu 2 þ was used as a positive control because this concentration is known to inhibit germination in lettuce (Sharma et al, 2009). Seed Germination Filter Paper (diameter 90 mm, grade 181, Whatman™) was fully soaked in each treatment solution and placed in a petri dish.…”

Section: Seed Germination Shoot and Root Elongation Assaysmentioning

confidence: 99%

“…To avoid water loss during incubation, the petri dishes were sealed with parafilm and kept in the dark for eight days. Crop seeds were incubated at 20°C (Sharma et al, 2009) while native seeds were incubated at 30°C (Mott, 1978). To compensate for water loss from evaporation due to higher temperature in the native species incubation, 1 ml of MilliQ water was added every two days.…”

Section: Seed Germination Shoot and Root Elongation Assaysmentioning

confidence: 99%

“…Discharge of NR from the textile dye industry is recognised as a serious environmental issue (Sharma et al, 2009;Zhou, 2001). In India, wastewater from the dye industry is often discharged without adequate treatment (Sharma et al, 2009). In China, a total organic dye concentration between 83 and 706 mg/l was recorded in wetlands near dye industries and between 12.3 and 456.2 mg/kg dry weight in surface soil (Zhou, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Since its earliest known use as a vital stain in 1894 (Koehring, 1930), NR has been broadly used as an intracellular pH indicator (Lamanna and McCracken, 1984), ecological marker (New, 1958), textile dye (Sharma et al, 2009;Zhou, 2001;Sawran et al, 2012), histological stain in cytotoxicity assays (Guerard et al, 2012) and recently as electron shuttles in microbial fuel cells for electricity generation (Park and Zeikus, 2000) and during reductive halogenation (Watanabe et al, 2009;Yee et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ecotoxicity of neutral red (dye) and its environmental applications

Kastury

Juhasz

Beckmann

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Seed Germination Shoot and Root Elongation Assaysmentioning

confidence: 99%

Section: Seed Germination Shoot and Root Elongation Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ecotoxicity of neutral red (dye) and its environmental applications

Kastury

Juhasz

Beckmann

et al. 2015

Ecotoxicology and Environmental Safety

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Redox Mediator‐Based Microbial Biosensors for Acute Water Toxicity Assessment: A Critical Review

et al. 2020

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Water toxicity assessment is of great importance for monitoring water quality and safeguarding ecological health. Microbial biosensors, which use microorganisms as the bioreceptor, offer a promising complementary method for water quality evaluation. The present Minireview has particular emphasis on microbial biosensors that use redox mediators as signal indicators and their performance for water acute toxicity assessment. The principles, fabrication methods, and recent developments of electrochemical microbial biosensors are systematically summarized. In addition, the application of microbial fuel cells in water biological oxygen demand and biotoxicity determination are also briefly introduced. The main hurdles that hinder the on‐site application of electrochemical microbial biosensors are discussed. With unceasing efforts on improvement of sensitivity and stability, electrochemical microbial biosensors are promising to play more crucial roles in water quality evaluation.

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Zebrafish embryo toxicity of anaerobic biotransformation products from the insensitive munitions compound 2,4‐dinitroanisole

Olivares

Sierra-Álvarez

Abrell

et al. 2016

Enviro Toxic and Chemistry

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2,4-dinitroanisole (DNAN) is an emerging insensitive munitions compound that readily undergoes anaerobic nitro-group reduction to 2-methoxy-5-nitroaniline (MENA) and 2,4-diaminoanisole (DAAN), followed by subsequent formation of unique azo-dimers. Currently there is scarce knowledge on the ecotoxicity of DNAN (bio)transformation products. In this work, mortality, development, and behavioral effects of DNAN (bio)transformation products were assessed using zebrafish (Danio rerio) embryos. We tested individual products, MENA and DAAN, as well as dimer and trimer surrogates. As pure compounds, 3-nitro-4-methoxyaniline and 2,2’-dimethoxy-4,4’-azodianiline caused significant effects with lowest observable effect levels (LOEL) at 6.4 µM on one or two developmental endpoints, respectively. The latter had six-additional significant developmental endpoints with LOELs of 64 µM. Based on light/dark swimming behavioral tests, DAAN (640 µM) caused reduction in swimming, suggestive of neurotoxicity. No significant mortality occurred (≤ 64 µM) for any of the individual compounds. However, metabolite mixtures formed during different stages of MENA (bio)transformation in soil were characterized using high-resolution mass spectrometry in parallel with zebrafish embryo toxicity assays, which demonstrated significant mortality during the onset of azo-dimer formation. Overall the results indicate that several DNAN (bio)transformation products cause different types of toxicity to zebrafish embryos.

show abstract

Monitoring toxicity of an azo dye methyl red and a heavy metal Cu, using plant and animal bioassays

Cited by 24 publications

References 20 publications

Ecotoxicity of neutral red (dye) and its environmental applications

Ecotoxicity of neutral red (dye) and its environmental applications

Redox Mediator‐Based Microbial Biosensors for Acute Water Toxicity Assessment: A Critical Review

Zebrafish embryo toxicity of anaerobic biotransformation products from the insensitive munitions compound 2,4‐dinitroanisole

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