Mono-homologous linear DNA recombination by the non-homologous end-joining pathway as a novel and simple gene inactivation method: a proof of concept study inDietziasp. DQ12-45-1b

Abstract

17Non-homologous end-joining (NHEJ) is critical for genome stability because of its roles in Therefore, this method may be a widely applicable genome editing tool for diverse prokaryotic 31 organisms, especially for non-model microorganisms. (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/300723 doi: bioRxiv preprint first posted online Apr. 13, 2018; IMPORTANCE 35The non-model gram-positive bacteria lack efficient genetic manipulation systems, but they 36 express genes encoding Ku and LigD. The NHEJ pathway in Dietzia sp. DQ12-45-1b was 37 evaluated and was used to successfully knockout eleven genes in the genome. Since bioinformatic 38 studies revealed that the putative genes encoding Ku and LigD ubiquitously exist in 39 phylogenetically diverse bacteria and archaea, the mono-homologous linear DNA recombination 40 by the NHEJ pathway could be a potentially applicable genetic manipulation method for diverse 41 non-model prokaryotic organisms.

Healthy Cattle Microbiome and Dysbiosis in Diseased Phenotypes

“…as a potential probiotic to inhibit Mycobacterium avium subsp. Paratuberculosis (MAP) and Johne's disease in vitro [153]. The susceptible calf, following MAP infection, had been administered probiotics to impede the development of Johne's disease.…”

Healthy Cattle Microbiome and Dysbiosis in Diseased Phenotypes

“…as potential probiotics, to inhibit Mycobacterium avium subsp. Paratuberculosis (MAP) and Johne's disease in vitro [153]. Probiotics had been administered to impede the development of Johne's disease, following MAP infection, in the susceptible calf.…”