Monoacylglycerol lipase from marine Geobacillus sp. showing lysophospholipase activity and its application in efficient soybean oil degumming

Li, Xuan; Wang, Weifei; Zhao, Zexin; Xu, Long; Yang, Bo; Lan, Dongming; Wang, Yonghua

doi:10.1016/j.foodchem.2022.134506

Cited by 12 publications

(1 citation statement)

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“…The presence of phospholipids in crude oils was sometimes considered to negatively affect the oil’s appearance and flavour (Liu et al, 2023). Since the oxidation of phospholipids has a substantial influence on the stability, shelf life, and quality of food oils, in food lipid production, degumming is often required to remove phospholipids from crude oils (Li et al, 2023).…”

Section: Resultsmentioning

confidence: 99%

EngineeringRhodosporidium toruloidesfor sustainable production of value-added punicic acid from glucose and wood residues

Wang,

Haddis,

Xiao

et al. 2024

Preprint

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Rhodosporidium toruloideshas emerged as a prominent candidate for producing single-cell oil from cost-effective feedstocks. In this study, the capability ofR. toruloidesto produce punicic acid (PuA), a representative plant unusual fatty acid, was investigated. The introduction of acyl lipid desaturase and conjugase (PgFADX) allowedR. toruloidesto accumulate 3.7% of total fatty acids as PuA. Delta-12 acyl lipid desaturase (PgFAD2) and diacylglycerol acyltransferase 2 were shown to benefit PuA production. The strain withPgFADXandPgFAD2coexpression accumulated 12% of its lipids as PuA from glucose, which translated into a PuA titer of 451.6 mg/L in shake flask condition. Utilizing wood hydrolysate as the feedstock, this strain produced 6.4% PuA with a titer of 310 mg/L. Taken together, the results demonstrated thatR. toruloidescould serve as an ideal platform for the production of plant-derived high-value conjugated fatty acid using agricultural and forestry waste as feedstock.

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Section: Resultsmentioning

confidence: 99%