Monoclonal Antibodies against Heat-Treated Muscle Proteins for Species Identification and End-Point Temperature Determination of Cooked Meats

Hsieh, Y-H. Peggy; Chen, F. C.; Djurdjevic, N.

doi:10.1007/978-1-4615-4731-0_20

Cited by 3 publications

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monoclonal antibodies (MAbs) are homogeneous populations of antibodies produced by cell‐fusion hybridoma technology. They have defined biological activities, consistent specificity, and unlimited production capability (Hsieh and Others 1999). Species‐specific MAbs raised against thermal‐stable flesh proteins can be used to construct inexpensive rapid (complete in minutes) field immunoassay test kits, enabling untrained inspectors or fisherman to verify the true species of a fish sample regardless of any heat treatment and whether it is raw, frozen, cooked, or canned.…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Monoclonal Antibodies Specific to Pangasius Catfish, Basa, and Tra

Gajewski¹,

Chen²,

Hsieh³

2009

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

Four IgG (subclass IgG1) class monoclonal antibodies (MAbs) strongly reactive to Asian farm-raised Pangasius catfish, tra (Pangasius hypophthalmus) and basa (Pangasius bocourti), have been developed. These MAbs were raised by immunizing an animal with thermal-stable crude sarcoplasmic protein extract of cooked tra. The MAbs were selected by screening hybridoma clones against more than 70 common fish and meat protein extracts. Two MAbs, T7E10 and T1G11, were found to be specific to the Asian Pangasius catfish, tra, and basa, with no cross-reactions with any of the common fish and meat species or with the food additive proteins (bovine serum albumin, soy proteins, milk proteins, egg proteins, and gelatin) tested. MAb T7E10 recognized 2 antigenic proteins (molecular weight approximately 36 and 75 kDa) in raw and cooked tra and basa extracts, while T1G11 bound to several proteins (molecular weight between 13 and 18 kDa) in tra and basa extracts. Two other MAbs, F7B8 and F1G11, recognized a common protein (36 KDa) and cross-reacted with all the fish extracts tested and with several mammalian species. These MAbs can be employed individually or in combination in various formats of immunoassays for rapid identification of Pangasius catfish, either raw or cooked. They can also be used to study the biological, biochemical, and physiological aspects of thermal-stable antigenic proteins. This is the first study identifying these thermal-stable antigenic proteins present in Pangasius catfish as species-specific biomarkers.

show abstract

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Monoclonal Antibodies Specific to Pangasius Catfish, Basa, and Tra

Gajewski¹,

Chen²,

Hsieh³

2009

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

show abstract

Animal Specification in Speciation

Ritter

Allred

2009

Molecular Biological and Immunological Techniques and Applications for Food Chemists

View full text Add to dashboard Cite

Rapid Immune Colloidal Gold Strip for Cetacean Meat Restraining Illegal Trade and Consumption: Implications for Conservation and Public Health

Chin

Chu

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

The consumption of cetacean meat is geographically common and often of undetermined sustainability. Besides, it can expose humans to contaminants and zoonotic pathogens. The illegality of possessing cetacean meat was likely under-reported in some countries due to lack of attention paid by the officials although DNA analysis of market products helped to show such practices. We developed two monoclonal antibodies against synthetic peptides of myoglobin (Mb) for constructing a rapid immune colloidal gold strip. Only cetacean Mb is capable of binding to both antibodies and presents positive signal while the Mb from other animals can bind only 1 of the antibodies and presents negative result. The strip for cetacean meat would be an applicable and cost-effective test for field inspectors and even the general public. It contributes to increase the reporting capacity and coverage of illegal cetacean meat possession, which has implications for global cetacean conservation and public health.

show abstract

Monoclonal Antibodies against Heat-Treated Muscle Proteins for Species Identification and End-Point Temperature Determination of Cooked Meats

Cited by 3 publications

References 70 publications

Production and Characterization of Monoclonal Antibodies Specific to Pangasius Catfish, Basa, and Tra

Production and Characterization of Monoclonal Antibodies Specific to Pangasius Catfish, Basa, and Tra

Animal Specification in Speciation

Rapid Immune Colloidal Gold Strip for Cetacean Meat Restraining Illegal Trade and Consumption: Implications for Conservation and Public Health

Contact Info

Product

Resources

About