Monoclonal antibodies against serogroup B salmonellae: production, characterisation and use in a sandwich ELISA

Abstract

Ten monoclonal antibodies (MAbs) against serogroup B salmonellae were obtained after immunisation of BALB/c mice with outer membrane proteins (OMPs) from Salmonella enterica serovar Typhimurium. Affinity constants, measured by enzyme-linked immunosorbent assay (ELISA), ranged from 8 )0 5 to 6)0 7 l mol (1 . Additivity ELISA demonstrated that most MAbs recognise different epitopes. ELISA determined the antigen specificity of the MAbs. The MAbs were more reactive with live Salmonella Typhimurium than with heattreated cells. Two MAbs were used to develop a sandwich ELISA for rapid detection of Salmonella Typhimurium in experimentally contaminated meats. Its detection limit was 10 5 CFU ml (1 and it was able to detect 1Á10 CFU in postenrichment broth from 25 g of beef and chicken meat samples. The sandwich ELISA developed was shown to be specific and sensitive for the detection of Salmonella Typhimurium in meat, and produced results comparable to the culture methods in 57 h.

Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms

“…Recently conformist PCR based assays for Salmonella detection in foods have been widely reported (Moreira et al, 2008;Ammar et al, 2010 andZowail et al, 2017). The stn gene is in attendance in all Salmonella serotypes and contained a unique sequence that considered as suitable PCR target for detection of Salmonella strains in field samples (Moore et al, 2007;Singh et al, 2017 andAmmar et al, 2019) The sequencing analysis and GC content of the stn gene of Salmonella isolates were an average of 53.3% which is a good prediction (Singh et al, 2017).…”

Outer Membrane Proteins and Efflux Pumps Mediated Multi-Drug Resistance in Salmonella: Rising Threat to Antimicrobial Therapy

“…For MDR and XDR Gram-negative infections, passive immunization generated by antibodies targeting OMPs was considered a favorable treatment strategy. 128 Moreira et al 145 showed that immunizing BALB/c mice with an OMP antigen of 38 kDa from S. Typhimurium produced 10 mAbs against serogroup B Salmonellae. As a result, antibodies to detect Salmonella Saint Paul, S. Typhimurium, and Salmonella Heidelberg in food samples have been developed to reduce the spread of Salmonellosis.…”

Monoclonal antibody-based cross-reactive sandwich ELISA for the detection of Salmonella spp. in milk samples

“…Most of these assays are specic to one serotype or one serogroup of Salmonella. [45][46][47] Linh Thuoc Tran and co-authors developed an ELISA to detect Salmonella spp. in foods using the recombinant H antigen.…”