Monoclonal Antibodies Against the Plant Cytokinin, <i>cis</i>-Zeatin Riboside

Banowetz, Gary M.

doi:10.1089/hyb.1993.12.729

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…Such methods were coupled with other chromatographic methods to analyze cZ derivatives from various sources, including potato tuber sprouts (Nicander et al ., 1995; Nicander et al ., 1993). The first monoclonal antibodies against a cis -derivative of a plant CK were developed by Banowetz (1993). Immunopurification methods coupled with LC-MS analysis of CKs are still commonly used today (Novak et al ., 2003) and contribute to the increasing number of publications regarding the levels of cZ and its derivatives as well as other CKs in tissues from various sources, including pea ( Pisum sativum ) roots (Stirk et al ., 2008); algae ( Chlorella minutissima , Stirk et al ., 2011); moss ( Physcomitrella patens ) (Lindner et al ., 2014; von Schwartzenberg et al ., 2007) and the plant pathogen Rhodococcus fascians (Pertry et al ., 2009).…”

Section: A Brief History Of the Analysis Of Cis-zeatin Derivativesmentioning

confidence: 99%

The role ofcis-zeatin-type cytokinins in plant growth regulation and mediating responses to environmental interactions

Schäfer

Brütting

Meza-Canales

et al. 2015

EXBOTJ

211

153

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Cytokinins (CKs) are well-established as important phytohormonal regulators of plant growth and development. An increasing number of studies have also revealed the function of these hormones in plant responses to biotic and abiotic stresses. While the function of certain CK classes, including trans-zeatin and isopentenyladenine-type CKs, have been studied in detail, the role of cis-zeatin-type CKs (cZs) in plant development and in mediating environmental interactions is less well defined. Here we provide a comprehensive summary of the current knowledge about abundance, metabolism and activities of cZs in plants. We outline the history of their analysis and the metabolic routes comprising cZ biosynthesis and degradation. Further we provide an overview of changes in the pools of cZs during plant development and environmental interactions. We summarize studies that investigate the role of cZs in regulating plant development and defence responses to pathogen and herbivore attack and highlight their potential role as 'novel' stress-response markers. Since the functional roles of cZs remain largely based on correlative data and genetic manipulations of their biosynthesis, inactivation and degradation are few, we suggest experimental approaches using transgenic plants altered in cZ levels to further uncover their roles in plant growth and environmental interactions and their potential for crop improvement.

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Section: A Brief History Of the Analysis Of Cis-zeatin Derivativesmentioning

confidence: 99%

The role ofcis-zeatin-type cytokinins in plant growth regulation and mediating responses to environmental interactions

Schäfer

Brütting

Meza-Canales

et al. 2015

EXBOTJ

211

153

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“…The HPLC conditions used in these studies permitted baseline resolution of the cis ‐ and trans ‐isomers of both zeatin and zeatin riboside but did not provide a clear separation of cis ‐Z and dihydrozeatin nor their ribosides (data not presented). In addition, the anti‐ cis ‐ZR mAb used in these studies exhibits significant cross‐reactivity toward dihydrozeatin and its riboside ( Banowetz 1993). Although previous studies failed to detect significant quantities of dihydrozeatin or its riboside in tuber extracts ( Suttle 1998a), a preliminary study was performed to verify that endogenous dihydrozeatin‐type cytokinins did not interfere with these analyses.…”

Section: Resultsmentioning

confidence: 99%

“…The methanolic eluates were dried, resuspended in a concentrated form and fractionated by C 18 ‐reverse‐phase HPLC using a binary 1% (v/v) acetic acid/acetonitrile gradient system. Aliquots of the appropriate fractions were then analyzed for cis ‐Z(R) content by mAb ELISA ( Banowetz 1993). Extraction efficiency was monitored by following the recovery of trans ‐[8‐ 14 C]‐zeatin (Sigma Chemical Co., St. Louis, MO, USA) added immediately after tissue homogenization.…”

Section: Methodsmentioning

confidence: 99%

Changes in cis‐zeatin and cis‐zeatin riboside levels and biological activity during potato tuber dormancy

Suttle

Banowetz

2000

Physiologia Plantarum

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20°C) prior to use, the rise in endogenous cis-Z was less The effects of postharvest storage duration and temperature on endogenous cis-zeatin (cis-Z) and cis-zeatin riboside (cis-dramatic and more protracted; increasing twofold after 53 days of storage. No change in cis-Z riboside content was ZR) levels in potato (Solanum tuberosum L.) tubers were observed in these tubers during this period. Dose-response determined in relation to tuber bud dormancy. The tubers used in these studies were completely dormant for at least 81 studies using either cis-Z or trans-Z demonstrated a time-dependent increase in cytokinin sensitivity during postharvest days of storage. Thereafter, tuber bud dormancy diminished storage. Immediately after harvest, dormant tubers were in-gradually and after 165 days of postharvest storage, the tubers were completely non-dormant. Immediately after har-sensitive to both zeatin isomers. Thereafter, tubers exhibited a dose-dependent increase in premature sprouting following vest, endogenous levels of cis-Z and cis-ZR were approxiinjection with either cytokinin isomer. After injection into mately 25 pmol (g fresh weight) − 1 and 8 pmol (g fresh dormant tubers, cis-[8-14 C]-zeatin was metabolized primarily weight) − 1 , respectively. In tubers exiting dormancy but stored at a growth-inhibiting temperature (3°C), endogenous to adenine/adenosine and cis-Z riboside. Seven days after injection, less than 10% of the recovered radioactivity was levels of cis-Z rose over threefold after 25 days of storage associated with trans-ZR. These results are consistent with a and remained elevated for the duration of the study. Levels of role for endogenous cis-Z (and its derivatives) in the regula-cis-ZR remained essentially constant during this same period. tion of potato tuber dormancy. In tubers transferred to a growth permissive temperature stored crop and result in financial losses to producers and processors in excess of several hundred million dollars annually.At the cellular level, tuber meristem dormancy is a result of an inhibition of cell division (Macdonald and Osborne 1988). Meristematic cells in the tuber buds (eyes) are arrested in the G 1 -phase of the cell cycle prior to the onset of DNA replication (Campbell et al. 1996). The resumption of bud growth following the termination of dormancy is accompanied by a large increase in DNA synthesis and in the number of cells traversing the cell cycle.The molecular processes regulating meristem dormancy in plants remain unknown (see Bewley 1997). However, there

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“…The immunoassays with these antibodies have detected as little as 5-10 pg of the homologous and structurally related cytokinins, when used in both competitive and non-competitive immunoassays. Those were the first monoclonal antibodies against plant cytokinins and can be useful for the purification of the 9-ribosides and 9-glucosides in plant samples [87,88].…”

Section: Cytokinin-binding Proteinsmentioning

confidence: 99%