Monoclonal antibodies as probes for defining cellular subsets in the bone marrow, thymus, bursa of Fabricius, and spleen of the chicken

Jeurissen, S.H.M.; Janse, E.M.; Ekino, Shigeo; Nieuwenhuis, Paul; Koch, G.; Boer, G. F. de

doi:10.1016/0165-2427(88)90110-9

Cited by 90 publications

(30 citation statements)

References 11 publications

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“…In some of previous studies (Thorbecke et al, 1957;Payne, 1971;Hodges, 1974;Jeurissen et al, 1988), it has been stated that the boundary between the white and red pulps was not distinguishable in poultry. On the other hand, the boundary between the white and red areas was distinguishable in some others (Starck and Riclefs, 1998;Causey Whittow, 2000).…”

Section: Discussionmentioning

confidence: 99%

Histologic and Histometric Examination of Spleen in Geese (Anser anser)

Bingöl¹,

Gülmez²,

Deprem³

et al. 2014

Atatürk Üniversitesi Vet. Bil. Derg.

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Abstract:The aim of this study was to examine the histometrical and histological structures of goose (Anser anser) spleen.Six healthy female geese were used as material. Tissue samples taken from the spleen were processed routinely, and were then stained with H&E, Crossman's Triple stain and Toluidine blue stain. The spleen surrounded by capsules composed of connective tissue and parts of the capsules were observed increasingly thinning into the spleen as trabeculae. The red pulp area was distinguishable from the white pulp inside the organ; further, the lymph follicles appeared clearly within the white pulp. Histometric measurements revealed that the thickness of the capsules surrounding the organ ranged from 18 to 28 µm. The average thickness of the capsules was measured as 22 µm. The average number of lymph follicles was found to be 2.4 in 1.07 mm 2 . The average width and length of the lymph follicles were measured as 113 µm and 144 µm, respectively.The average diameter of the mast cells was found to be 6 µm. The average number of mast cells was found to be 1.4 in 1.07 mm 2 . Although the histological structures of the geese spleens seemed very similar to those of other animals in several respects, but some specific properties of geese spleens being more similar to that of mammalians were also observed.

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Section: Discussionmentioning

confidence: 99%

Histologic and Histometric Examination of Spleen in Geese (Anser anser)

Bingöl¹,

Gülmez²,

Deprem³

et al. 2014

Atatürk Üniversitesi Vet. Bil. Derg.

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“…Availability of a reliable immunostaining of microglial cells in the chick would be a valuable tool for investigators of this model system. The possibility that microglia in the chick might express CD45, as occurs in mammals, and the commercial availability of the HIS-C7 monoclonal antibody (Jeurissen et al 1988a), which recognizes the CD45 homolog in chicks, led us to use this antibody in the chick CNS to test its utility as an immunomarker for microglia in this species. Immunocytochemical analysis of developing and adult brains and retinas showed that HIS-C7 recognizes macrophages and all forms of microglial cells, including mature ramified ones, and can therefore be used to identify microglial cells in the chick.…”

Section: S U M M a R Ymentioning

confidence: 99%

“…Sections from chicken specimens were immunostained with monoclonal antibodies HIS-C7, which recognize a CD45 homolog in chickens (Jeurissen et al 1988a); CVI-68.1, which labels chicken mononuclear phagocytes (Jeurissen et al 1988b); and Lep-100, which labels a glycoprotein of the membrane of lysosomes (LippincotSchwartz and Fambrough 1986).…”

Section: Antibodies and Immunocytochemistrymentioning

confidence: 99%

Specific Immunolabeling of Brain Macrophages and Microglial Cells in the Developing and Mature Chick Central Nervous System

Cuadros

Santos

Martín‐Oliva

et al. 2006

J Histochem Cytochem.

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The present study showed that the HIS-C7 monoclonal antibody, which recognizes the chick form of CD45, is a specific marker for macrophages/microglial cells in the developing and mature chick central nervous system (CNS). HIS-C7-positive cells were characterized according to their morphological features and chronotopographical distribution patterns within developing and adult CNS, similar to those of macrophages/microglial cells in the quail CNS and confirmed by their histochemical labeling with Ricinus communis agglutinin I, a lectin that recognizes chick microglial cells. Therefore, the HIS-C7 antibody is a valuable tool to identify brain macrophage and microglial cells in studies of the function, development, and pathology of the chick brain. CD45 expression differed between chick microglia (as revealed with HIS-C7 antibody) and mouse microglial cells (as revealed with an antibody against mouse form of CD45). Thus, a discontinuous label was seen on mouse microglial cells with the anti-mouse CD45 immunostaining, whereas the entire surface of chick microglial cells was labeled with the anti-chick CD45 staining. The functional relevance of these differences between species has yet to be determined.

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“…Titrated sera were incubated for 1 h at room temperature in a twofold dilution. The isotype-specific responses were determined with mouse monoclonal antibodies CVI-ChIgM-59.7, specific for chicken IgM, and CVI-ChIgG-47.3, specific for chicken IgG (Bianchi et al, 1990;Jeurissen et al, 1988). Detection was performed with rabbit-anti-mouse-HRPO (DAKO A/S, Glostrup, Denmark) and the substrate tetramethylbenzidine (0.1 mg/ml) and H 2 O 2 (0.005%, v/v).…”

Section: Specific T-cell Proliferationmentioning

confidence: 99%