Monoclonal Antibodies: Clinical Utility and the Misunderstood Epitope

Keller, R.; Milson, Thomas J.; Janicek, Karen M.; Patrick, Catherine W.

doi:10.1093/labmed/15.12.795

Cited by 5 publications

(3 citation statements)

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“…A variety of highly specific monoclonal antibodies is now commercially available against multiple markers of T-lymphocytes, B-lymphocytes, histiocytes, and myeloid cells (3,5,10,(22)(23)(24)(25)(26). The antibodies that we employ routinely are listed in Table I.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, scattered (primarily helper) T-cells are normally identified within the follicular centers. The interfollicular T-cells contain a mixture of helper and suppressor subsets, in a ratio of approximately 2 to 1 (3,22,23,25). Hyperplasia of B-or T-cells results in an expansion of the zones that these cells normally occupy.…”

Section: Lmmunoarchitecture Of Normal Reactive and Neoplastic Lymphmentioning

confidence: 99%

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Diagnostic immunohistochemistry of lymphoma and related disorders: Practical aspects of frozen section technique and interpretation

Robbins

1987

Clinical Laboratory Analysis

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The diagnosis and classification of malignant lymphomas increasingly relies upon analysis of lymphocyte markers. Immunohistochemical techniques applied to frozen sections of lymphoid tissue have the advantage of allowing simultaneous evaluation of both immunologic phenotype and histologic/cytologic features. This paper describes the methodology currently employed in the Scripps institution for lymphocyte marker studies on tissue biopsies, including avidin‐biotin complex (ABC) immunoperoxidase and direct immunoperoxidase techniques. Emphasis is placed on technical aspects of the procedures and pitfalls in interpretation. A detailed listing of monoclonal antibodies against lymphocyte markers is provided, including technical features and important cross reactivities. The results of 60 cases analyzed over a one‐year period are presented. Essential immunophenotypic features of lymphoid hyperplasia, B‐cell lymphomas, T‐cell lymphomas, Hodgkin's disease, thymomas, and undifferentiated carcinoma are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Lmmunoarchitecture Of Normal Reactive and Neoplastic Lymphmentioning

confidence: 99%

Diagnostic immunohistochemistry of lymphoma and related disorders: Practical aspects of frozen section technique and interpretation

Robbins

1987

Clinical Laboratory Analysis

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“…Recognition that some surface antigens were expressed across multiple lineages and/or subsets denoted the importance of multifluorescence probe analysis [7], Among the dyes which may be utilized with argon ion lasers, fluorescein isothiocyanate, phyco-bilierythrin and allophycocyanin derivatives currently appear to be most popular. The results of two and three fluorochrome probe simultaneous analysis combined with func tional studies serve to expand our under standing of lineage and subset relationships [8,9], The lymphoid elements were the first to be studied with this new form of biologic dissec tion; however, by the early 1980s monoclonal antibodies were available against surface membrane antigens of monocytes, granulo cytes, erythrocytes, megakaryocytes, platelets and solid tumors.…”

Section: Evolution Of Clinical Cytometry' and Immunophenotypingmentioning

confidence: 99%

Clinical Cytometry and Immunophenotyping

Patrick

Ash

Libnoch

et al. 1987

Path Immunopathol Res

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Flow cytometry in the diagnosis of lymphomas

Parker

1988

Cytometry

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Because of recent advances in instrumentation, immunological reagents, and methods for measuring a variety of cell constituents and functions, there has been increasing interest in clinical applications of flow cytometry. Several commercially available flow cytometers and a growing list of monoclonal antibodies make it feasible for clinical laboratories to apply this technology for phenotyping leukocytes in leukemias, lymphomas, immunological disorders, transplantation, and infectious diseases. The ability to measure cell constituents such as DNA, RNA, proteins, enzymes, and hormone receptors has added to the list of applications. Use in conjunction with simultaneous phenotyping with monoclonal antibodies makes such measurements even more valuable. Phenotyping specimens from a large number of patients with lymphomas and leukemias has demonstrated the value of flow cytometry in diagnosis and patient management.

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Monoclonal Antibodies: Clinical Utility and the Misunderstood Epitope

Cited by 5 publications

References 0 publications

Diagnostic immunohistochemistry of lymphoma and related disorders: Practical aspects of frozen section technique and interpretation

Diagnostic immunohistochemistry of lymphoma and related disorders: Practical aspects of frozen section technique and interpretation

Clinical Cytometry and Immunophenotyping

Flow cytometry in the diagnosis of lymphomas

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