1991
DOI: 10.1007/bf00208241
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Monoclonal antibodies directed to phytochrome from green leaves of Avena sativa L. cross-react weakly or not at all with the phytochrome that is most abundant in etiolated shoots of the same species

Abstract: Seven monoclonal antibodies (MAbs) have been prepared to phytochrome from green oat (Avena sativa L. cv. Garry) leaves. One of these MAbs (GO-1) cross-reacts with apoprotein of the phytochrome that is most abundant in etiolated oat shoots as assessed by immunoblot assay of fusion proteins expressed in Escherichia coli. The epitope for this MAb is located between amino acids 618 and 686 in the primary sequence of type 3 phytochrome (Hershey et al. 1985, Nucleic Acids Res. 13, 8543-8559), which is one of the pre… Show more

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Cited by 13 publications
(20 citation statements)
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“…Computer predictions of surface probability suggest that the region between roughly amino acids S620 and E690 are located within the interior of phytochrome. More convincingly, this region is not readily accessible to proteases (Grimm et al, 1988), and a monoclonal antibody, GO-1, specific to this region is only able to recognize phytochrome by immunoblotting, and not by ELISA (Pratt et al, 1991). Sequences on either side of V623-S673 are modified by a variety of agents when phytochrome is in the Pfr conformation.…”
Section: Structure Prediction Of the Dimerization Regionsmentioning
confidence: 99%
See 3 more Smart Citations
“…Computer predictions of surface probability suggest that the region between roughly amino acids S620 and E690 are located within the interior of phytochrome. More convincingly, this region is not readily accessible to proteases (Grimm et al, 1988), and a monoclonal antibody, GO-1, specific to this region is only able to recognize phytochrome by immunoblotting, and not by ELISA (Pratt et al, 1991). Sequences on either side of V623-S673 are modified by a variety of agents when phytochrome is in the Pfr conformation.…”
Section: Structure Prediction Of the Dimerization Regionsmentioning
confidence: 99%
“…Also, the existente of two or more different types of phytochrome raises the possibility of heterodimer formation. Pratt et al (1991) have recently provided biochemical evidence that such heterodimers do indeed form in solution. For these reasons, we have focused our efforts on identification and characterization of regions of the protein involved in dimerization.…”
Section: Introductionmentioning
confidence: 99%
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“…Pea-25 is an antibody directed to phytochrome from etiolated pea shoots (7,8). Oat-16 and GO-7 were raised against phytochromes from etiolated and GO shoots, respectively (9,22,28).…”
Section: Plant Materialsmentioning
confidence: 99%