Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid

Mertens, Ralf; Deus-Neumann, Brigitte; Weiler, Elmar W.

doi:10.1016/0014-5793(83)80980-6

Cited by 172 publications

(96 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in Figure 3 such deviations could not be observed in the test extract. Together with the data shown in Figure 2, the results show that combination of separation techniques with ELISA (17) and IAA (18). In these studies, it became clear that the selection of mAB with specificities rivaling or even surpassing those of antisera is possible.…”

Section: -(2-ethylhexylamino)-2-methylpyrollo-[23-d]-pyrimidine and supporting

confidence: 57%

“…In these studies, it became clear that the selection of mAB with specificities rivaling or even surpassing those of antisera is possible. It was furthermore shown that, by employing appropriate screening protocols, the selection of high affinity mAB is possible (17,18). Both specificity and affinity need to be maximized in order to allow for optimum immunoassay performance.…”

Section: -(2-ethylhexylamino)-2-methylpyrollo-[23-d]-pyrimidine and mentioning

confidence: 99%

“…Due to their extreme usefulness as standardizable reagents (22) monoclonal antibodies specific for plant growth regulators are being developed (17,18). In this paper we report on the production of mAB with high affinity and specificity for ZR and DHZR, their free bases and phosphate esters.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Monoclonal Antibodies to Plant Growth Regulators

Eberle¹,

Arnscheidt²,

Klix³

et al. 1986

Plant Physiol.

View full text Add to dashboard Cite

show abstract

Section: -(2-ethylhexylamino)-2-methylpyrollo-[23-d]-pyrimidine and supporting

confidence: 57%

Section: -(2-ethylhexylamino)-2-methylpyrollo-[23-d]-pyrimidine and mentioning

confidence: 99%

See 1 more Smart Citation

Monoclonal Antibodies to Plant Growth Regulators

Eberle¹,

Arnscheidt²,

Klix³

et al. 1986

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…The ELISA was performed as described by Walker-Simmons (28) with the monoclonal antibody of Mertens et al (11), which was purchased from Idetek (San Bruno, CA). The ABA-4'-conjugate was prepared according to Weiler (29).…”

Section: Growth Of Plantsmentioning

confidence: 99%

On the Role of Abscisic Acid and Gibberellin in the Regulation of Growth in Rice

1992

View full text Add to dashboard Cite

show abstract

“…Levels of endogenous abscisic acid were determined for replicate batches of five embryos by ELISA, using an abscisicacid-specific monoclonal antibody developed specifically for ELISA [20] and obtained from Idetek Inc. (San Bruno, CA, USA). The procedures followed for the extraction of abscisic acid and the determination of its concentration by ELISA have been comprehensively detailed elsewhere [21].…”

Section: Invnunoassuy Qf Uhscisic Cicidmentioning

confidence: 99%

Osmotic stress and abscisic acid induce expression of the wheat Em genes

Morris

Kumar

Bowles

et al. 1990

European Journal of Biochemistry

121

View full text Add to dashboard Cite

The early-methionine-labelled (Em) polypeptide is the single most abundant cytosolic protein of dry wheat embryos. It is encoded by messenger RNA which accumulates during the later (maturation) stages of embryogenesis. The accumulation of Em mRNA can be induced in isolated developing embryos, in culture, by the application of the plant growth regulator, abscisic acid, which prevents precocious germination.Precocious germination is also inhibited by the culture of embryos under conditions of osmotic stress when accumulation of Em mRNA is induced. This induction occurs in the absence of any significant increase in the endogenous levels of embryonic abscisic acid although there is a requirement for the continued presence of the growth regulator. Additionally, expression of Em genes can be repeated during early germination, if imbibing embryos are subjected to osmotic stress. Induction of Em-gene expression by osmotic stress is consistent with the proposed role of the Em polypeptide in mediating the remarkable tolerance of cereal embryos to the programmed desiccation undergone during their maturation Embryonic development in cereals produces a differentiated embryo held within the developing grain in a developmentally arrested condition, whilst reserves within the endosperm continue to accumulate. The arrested embryo undergoes a period of maturation, during which characteristic changes in the pattern of gene expression occur within it. The maintenance of embryonic arrest and the associated accumulation of a maturation-specific subset of gene products is believed to be controlled by the plant growth regulator, abscisic acid: embryos which are removed from immature grains prior to the onset of maturation and cultured in the presence of abscisic acid do not germinate precociously, but accumulate maturation-specific proteins and mRNA [l -31.In the final stages of maturation, dehydration causes the cessation of metabolic activity within the embryo. Additionally, the developmental programme of the grain becomes radically altered, so that upon subsequent imbibition of water, the previously arrested mature embryo germinates. The maturation-specific gene products within the embryo disappear and the expression of a new subset of germination-specific genes occurs [4, 51. It is thought that the developmental switch effected by dehydration is due in part to the decline of endogenous abscisic acid during the last period of maturation. However, dehydration to a moisture content of z 5% fresh mass also appears to reduce the sensitivity of the embryo to inhibition of germination by exogenously applied abscisic acidIn recent years, a number of the abscisic-acid-related maturation-specific gene products of cereal embryos have been identified and characterised in some detail. The maintenance 151.Correspandence to A. C. Cuming, Department of Genetics, University of Leeds, Leeds LS2 9JT, EnglandAbbreviutions. dpa, days post-anthesis; Em, early-methioninelabelled polypeptide; norflurazon, 4-chloro-5-(methylamino)-2-(a,ct,a,-trifluoro-rr?-...

show abstract

Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid

Cited by 172 publications

References 12 publications

Monoclonal Antibodies to Plant Growth Regulators

Monoclonal Antibodies to Plant Growth Regulators

On the Role of Abscisic Acid and Gibberellin in the Regulation of Growth in Rice

Osmotic stress and abscisic acid induce expression of the wheat Em genes

Contact Info

Product

Resources

About