Monoclonal Antibodies Reacting Specifically with <i>Francisella</i> sp.

Greiser‐Wilke, I.; Soiné, Christiane; Moennig, V.

doi:10.1111/j.1439-0450.1989.tb00650.x

Cited by 13 publications

(16 citation statements)

References 27 publications

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“…The IgG1 monoclonal antibody (MAb) 11/1/6 was produced by myeloma cells fused with mouse (BALB/c) spleen cells after immunization with F. tularensis ATCC 6223 as previously described (21). No cross-reactivity with a wide range of gram-negative bacteria was observed (22).…”

Section: Methodsmentioning

confidence: 99%

“…Preparations of outer membrane antigens can be applied to several methodological platforms such as enzyme-linked immunosorbent assays (ELISA), microagglutination, and Western blotting (5,6,8). Assays based on LPS as a capture antigen, with different approaches to purification and detection, have been described previously (11,20,21,37). These preparations have yielded high specificity for Francisella type A and B strains, the assays are easy to carry out, and antigens were stable over a long period (22).…”

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confidence: 99%

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Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Porsch-Özçürümez

Kischel

Priebe

et al. 2004

Clin Vaccine Immunol

106

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Porsch-Özçürümez

Kischel

Priebe

et al. 2004

Clin Vaccine Immunol

106

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“…The MAbs directed against F. tularensis had been developed as described previously (8). Selected MAbs were produced in serum-free medium at concentrations of approximately 500 to 1,000 g/ml with a Miniperm bioreactor (Heraeus, Hanau, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Two specific murine MAbs to F. tularensis (8), Ft-11 (IgG1) and Ft-27 (IgM), were utilized for the cELISA. Both MAbs reacted strongly with the LPS of F. tularensis as shown by Western blot analysis (Fig.…”

Section: Methodsmentioning

confidence: 99%

Detection of Francisella tularensis in Biological Specimens Using a Capture Enzyme-Linked Immunosorbent Assay, an Immunochromatographic Handheld Assay, and a PCR

Grunow

Splettstoesser

McDonald³

et al. 2000

Clin Diagn Lab Immunol

139

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The early detection of Francisella tularensis, the causative agent of tularemia, is important for adequate treatment by antibiotics and the outcome of the disease. Here we describe a new capture enzyme-linked immunosorbent assay (cELISA) based on monoclonal antibodies specific for lipopolysaccharide (LPS) of Francisella tularensis subsp. holarctica and Francisella tularensis subsp. tularensis. No cross-reactivity with Francisella tularensis subsp. novicida, Francisella philomiragia, and a panel of other possibly related bacteria, including Brucella spp., Yersinia spp., Escherichia coli, and Burkholderia spp., was observed. The detection limit of the assay was 10 3 to 10 4 bacteria/ml. This sensitivity was achieved by solubilization of the LPS prior to the cELISA. In addition, a novel immunochromatographic membrane-based handheld assay (HHA) and a PCR, targeting sequences of the 17-kDa protein (TUL4) gene of F. tularensis, were used in this study. Compared to the cELISA, the sensitivity of the HHA was about 100 times lower and that of the PCR was about 10 times higher. All three techniques were successfully applied to detect F. tularensis in tissue samples of European brown hares (Lepus europaeus). Whereas all infected samples were recognized by the cELISA, those with relatively low bacterial load were partially or not detected by PCR and HHA, probably due to inhibitors or lack of sensitivity. In conclusion, the HHA can be used as a very fast and simple approach to perform field diagnosis to obtain a first hint of an infection with F. tularensis, especially in emergent situations. In any suspect case, the diagnosis should be confirmed by more sensitive techniques, such as the cELISA and PCR.Within the genus Francisella there are two known species, Francisella tularensis and Francisella philomiragia, which have a 16S rRNA gene sequence similarity of more than 98% (6). F. tularensis, a gram-negative, small (0.2 to 0.7 by 0.2 m) facultative intracellular bacterium,

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“…Successful vaccination for each pathogen was tested by ELISA and immunoblot. Finally, different mouse monoclonal antibodies (MAbs) recognizing different epitopes of F. tularensis LPS were tested in all four assays (12).…”

Section: Sera Used For Test Evaluationmentioning

confidence: 99%

Evaluation of an Immunochromatographic Test for Rapid and Reliable Serodiagnosis of Human Tularemia and Detection ofFrancisella tularensis-Specific Antibodies in Sera from Different Mammalian Species

et al. 2010

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Tularemia is a highly contagious infectious zoonosis caused by the bacterial agent Francisella tularensis.Serology is still considered to be a cornerstone in tularemia diagnosis due to the low sensitivity of bacterial culture and the lack of standardization in PCR methodology for the direct identification of the pathogen. We developed a novel immunochromatographic test (ICT) to efficiently detect F. tularensis-specific antibodies in sera from humans and other mammalian species (nonhuman primate, pig, and rabbit). This new tool requires none or minimal laboratory equipment, and the results are obtained within 15 min. When compared to the method of microagglutination, which was shown to be more specific than the enzyme-linked immunosorbent assay, the ICT had a sensitivity of 98.3% (58 positive sera were tested) and a specificity of 96.5% (58 negative sera were tested) on human sera. On animal sera, the overall sensitivity was 100% (22 positive sera were tested) and specificity was also 100% (70 negative sera were tested). This rapid test preferentially detects IgG antibodies that may occur early in the course of human tularemia, but further evaluation with human sera is important to prove that the ICT can be a valuable field test to support a presumptive diagnosis of tularemia. The ICT can also be a useful tool to monitor successful vaccination with subunit vaccines or live vaccine strains containing lipopolysaccharide (e.g., LVS) and to detect seropositive individuals or animals in outbreak situations or in the context of epidemiologic surveillance programs in areas of endemicity as recently recommended by the World Health Organization.

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Monoclonal Antibodies Reacting Specifically with Francisella sp.

Cited by 13 publications

References 27 publications

Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Comparison of Enzyme-Linked Immunosorbent Assay, Western Blotting, Microagglutination, Indirect Immunofluorescence Assay, and Flow Cytometry for Serological Diagnosis of Tularemia

Detection of Francisella tularensis in Biological Specimens Using a Capture Enzyme-Linked Immunosorbent Assay, an Immunochromatographic Handheld Assay, and a PCR

Evaluation of an Immunochromatographic Test for Rapid and Reliable Serodiagnosis of Human Tularemia and Detection ofFrancisella tularensis-Specific Antibodies in Sera from Different Mammalian Species

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