Monoclonal antibodies specific to haemocytes of black tiger prawn Penaeus monodon

Production and characterization of monoclonal antibodies to the hemocytes of mud crab, Scylla serrata

“…Although low level of reactivity was observed in semigranular cells, MAbs did not show any reaction with hyaline hemocytes. In terms of reactivity to particular hemocytes, the MAbs produced in the present study showed similarity with the MAbs produced against hemocytes of P. monodon by Rodriguez et al (1995), Sung et al (1999) and Winotaphan et al (2005). It was also observed that hyaline cells constitute 60% of the total hemocyte population of S. serrata.…”

“…This suggests that most of the proteins stored in the hemocytes may be released into the hemolymph as reported in the case of penaeid shrimps (Rodriguez et al, 1995;Sung et al, 1999;Van de Braak et al, 2001). However, in the case of P. monodon, all the MAbs generated showed reactivity only to hemocyte proteins (Winotaphan et al, 2005). In the present study also, six of the hybridomas reacted specifically with hemocyte proteins (apparent molecular weights of 39 and 255 kDa).…”

“…A similar approach has been adopted for many crustaceans, especially penaeid shrimp (Rodriguez et al, 1995;Sung et al, 1999;Van de Braak et al, 2000;Sung and Sun, 2002;Zhang et al, 2004;Winotaphan et al, 2005). However, there is no report of the antigenic characterization of hemocytes using MAbs in any of the crab species.…”

Pathogenesis of a Thai strain of white spot syndrome virus (WSSV) in juvenile, specific pathogen-free Litopenaeus vannamei

“…Double stainings were performed to characterize infected hemocytes. Here, a first incubation was made for 1 h at 37°C (1:70 dilution in PBS or 0.02 mg ml -1 solution) with hemocyte markers from Penaeus monodon (Winotaphan et al 2005), or with wheat germ agglutinin (WGA) labeled with FITC (L4895 Sigma) (Martin et al 2003). Cytospins were washed and incubated with 0.02 mg ml -1 goat anti-mouse IgG-FITC for 1 h at 37°C.…”

Classification of haematopoietic cells and haemocytes in Chinese prawn Fenneropenaeus chinensis

“…Cytochemical characterisations including lysosomes, cytoplasmic contents and granule enzymes were also attempted to provide information on the function of and relationship among different haemocyte types [4]. Monoclonal antibodies (mAbs) reacting with various proteins of haemoctyes have been obtained in Penaeus monodon [5,6], and more specific mAbs were produced to classify all three types of P. monodon haemocytes recently [7].…”