Monoclonal antibodies to human amnion

Hsi, Bae‐Li; Yeh, Chang-Jing G.

doi:10.1016/0165-0378(86)90021-5

Cited by 46 publications

(16 citation statements)

References 16 publications

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“…Monoclonal antibody GB3 raised against amnion epithelium (Hsi and Yeh, 1986) reacts with human laminin-5 (Verrando et al, 1987). Monoclonal antibody 6F12 (generous gift from Prof. R.E.…”

Section: Experimental Procedures Antibodiesmentioning

confidence: 99%

Developmental expression of laminin-5 and HD1 in the intestine: Epithelial to mesenchymal shift for the laminin γ2 chain subunit deposition

et al. 1996

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“…Monoclonal antibody GB3 raised against amnion epithelium (Hsi and Yeh, 1986) reacts with human laminin-5 (Verrando et al, 1987). Monoclonal antibody 6F12 (generous gift from Prof. R.E.…”

Section: Experimental Procedures Antibodiesmentioning

confidence: 99%

Developmental expression of laminin-5 and HD1 in the intestine: Epithelial to mesenchymal shift for the laminin γ2 chain subunit deposition

et al. 1996

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“…Recently, the use of sev eral monoclonal antibodies, including GB3 [9,10], 19- 48 Shimizu/Onodcra/Ikcda/Ogawa/Suzumori/ Nishikawa…”

Section: Discussionmentioning

confidence: 99%

“…Of 15 patients with EB referred to us, 3 were confirmed to have gravis JEB, because they lacked GB3 antigen at the epidermal basement membrane zone (BMZ) defined by GB3 monoclonal antibody [6,9,10]. GB3 monoclonal antibody defines a widespread defect of several basement membranes and a kératinocyte dysfunction in patients with gravis JEB [9].…”

Section: Introductionmentioning

confidence: 99%

Prenatal Diagnosis of Epidermolysis bullosa: First Successful Trial in Asia

Shimizu

Onodera²,

Ikeda³

et al. 1994

Dermatology

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Prenatal diagnosis of epidermolysis bullosa (EB) using fetal skin biopsy specimens has been successfully performed in Europe and America, but this technique has not previously been attempted in Asia. For the first time in Asia, we attempted to make a prenatal diagnosis of EB in a high-risk fetus by fetal skin biopsy. A skin biopsy was obtained from the fetus at risk of gravis type junctional epidermolysis bullosa of Herlitz. The biopsy specimen was studied by electron microscopy and immunohistochemistry using various monoclonal antibodies against the epidermal basement membrane zone (BMZ). There were no ultrastructural abnormalities in the BMZ, including the hemidesmosomes. Indirect immunofluorescence showed normal expression of GB3 antigen. The pregnancy was continued, and a normal, healthy infant was born. The prenatal diagnosis of epidermolysis bullosa is now available in Tokyo. This clinical diagnostic service is available to families from various parts of Asia.

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“…GB9 and GB11 were used for this study. Their production and their reactivities to hu man amnion and other extra-embryonic tissues were detailed by Hsi and Yeh [7], Undiluted supernatant fluids of the hybridoma cell lines were used. The cul ture medium of P3-NSl-Ag4-l (NS-1) cell line was used as a control.…”

Section: Antibodiesmentioning

confidence: 99%

“…However, due to the lack of specific antibodies to the ocular tissues, us ing antigens as cell differentiation markers to study eye development has rarely been performed. This condition has been im proved with the development of the mono clonal antibody technology [6], Recently, three mouse monoclonal antibodies GB4, GB9 and GB11 which were raised against human amnion [7] were found to react spe cifically with some structures in the anterior segment and the lens of the rabbit eye [8], GB4 reacted with the conjunctival epithe lium and the subcapsular epithelium of the lens; GB9 was positive only with the corneal epithelium; and GB11 mainly reacted with the pigmented epithelial cells of the ciliary processes. In this report, we undertook the study of rabbit eye embryogenesis using these three monoclonal antibodies.…”

Section: Introductionmentioning

confidence: 99%

Expression of the Antigens Recognized by Monoclonal Antibodies to Human Amnion (GB4, GB9, GB11) on the Rabbit Eye during Embryonic Development

et al. 1986

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Three monoclonal antibodies to human amnion (GB4, GB9 and GB11) which recognized well-defined structures of the adult rabbit eye were used to study the embryonic eye development. The reactivity of GB4 could be identified on the migrating neural crest cells under the corneal epithelium at 2 weeks of gestation. At 3 weeks of gestation, the conjunctival epithelium, corneal epithelium and corneal endothelium reacted with GB4. In the newborn rabbit, the corneal epithelium was reactive with GB9 instead of GB4; corneal endothelium was positive with GB4 at this stage, but gradually became negative in the adult rabbit; the subcapsular epithelium of the lens was recognized by GB4 when it changed from pseudostratified to simple cuboidal epithelium. During early embryonic life, GB11 was positive on the external retinal layer of the optic cup and lens vesicle. In the adult, GB11 reacted only with the pigmented epithelium of the ciliary processes. The results of this study demonstrated that the expression of antigens recognized by GB4, GB9 and GB11 could be mapped according to the epithelial differentiation of the rabbit eye during embryogenesis.

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Monoclonal antibodies to human amnion

Cited by 46 publications

References 16 publications

Developmental expression of laminin-5 and HD1 in the intestine: Epithelial to mesenchymal shift for the laminin γ2 chain subunit deposition

Developmental expression of laminin-5 and HD1 in the intestine: Epithelial to mesenchymal shift for the laminin γ2 chain subunit deposition

Prenatal Diagnosis of Epidermolysis bullosa: First Successful Trial in Asia

Expression of the Antigens Recognized by Monoclonal Antibodies to Human Amnion (GB4, GB9, GB11) on the Rabbit Eye during Embryonic Development

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