Monoclonal Antibody in the Identification of Haemophilus Somnus

Abstract

Abstract. Monoclonal antibody in the identification ofCoagglutination tests were performed using a monoclonal antibody coagglutination assay. The monoclonal reagent was produced by incubating Cowan strain Staphylococcus aureus suspension, used as a source of crude protein A, with mouse ascitic fluid monoclonal antibody or goat anti-H. somnus hyperimmune serum. Bacteria to be tested were suspended at a concentration of 4.5 x 10 9 cells/ml. The coagglutination test was performed by the addition of 50 µ1 of the monoclonal reagent to 50 µ1 of the bacterial suspension on a glass plate and manual rotation for 2-3 minutes.The coagglutination assay using Cowan strain Staphylococcus aureus protein A, coupled with the monoclonal antibody, agglutinated 10 different H. somnus isolates. The antibody reagent did not coagglutinate with Actinobacillus suis, A. equuli, Pasteurella haemolytica, P. multocida, or P. pneumotropica under similar test conditions.

Identification and characterization of outer membrane proteins of Pasteurella multocida serotype D by using monoclonal antibodies

“…(i) Preparation of OMPs. The method of Thomson et al (32) was followed for the preparation of OMPs of P. multocida P210, 3001, and 1703, with some modifications. The WC was disrupted in a French press at 20,000 b/in 2 in 1 mM EDTA-20 mM Tris HCl (pH 7.8).…”