Serological characterization of Actinobacillus pleuropneumoniae serotype 2 strains by using polyclonal and monoclonal antibodies

Actinobacillus pleuropneumoniae is an important pig pathogen that is responsible for swine pleuropneumonia, a highly contagious respiratory infection. Knowledge of the importance, composition and structural determination of the major antigens involved in virulence provides crucial information that could lead to the development of a rationale for the production of specific serodiagnostic tools as well as vaccine development. Thus, efforts have been devoted to study mainly A. pleuropneumoniae virulence determinants with special emphasis on the Apx toxins (for A. pleuropneumoniae RTX toxins). In comparison, little attention has been given to the surface polysaccharides, which include capsular polysaccharides (CPS) and cell-wall lipopolysaccharides (LPS). Here, we review current knowledge on CPS and LPS of A. pleuropneumoniae used as diagnostic tools to monitor the infection and as immunogens for inclusion in vaccine preparations for animal protection.

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Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

Dubreuil

Jacques

Mittal

et al. 2000

Anim. Health. Res. Rev.

114

111

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Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains

et al. 2008

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Dealing with unexpected Actinobacillus pleuropneumoniae serological results

Broes,

Martineau,

Gottschalk

2007

JSHAP

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Serological testing is widely used to monitor swine herds for Actinobacillus pleuropneumoniae (APP). Several serological tests are presently used, most often the complement fixation test, the long-chain lipopolysaccharide enzyme-linked immunosorbent assay (ELISA), and the ApxIV ELISA. Serological testing occasionally generates ambiguous results. In such situations, bacterial isolation and polymerase chain reaction testing must be used in order to accurately define the presence or absence of APP. Examples of unexpected serological results and the eventual means of establishing herd APP status are illustrated by means of 10 cases that occurred in European and North American herds.

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Serological characterization of Actinobacillus pleuropneumoniae serotype 2 strains by using polyclonal and monoclonal antibodies

Cited by 4 publications

References 28 publications

Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

Actinobacillus pleuropneumoniaesurface polysaccharides: their role in diagnosis and immunogenicity

Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains

Dealing with unexpected Actinobacillus pleuropneumoniae serological results

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