Monoclonal antibody-mediated clean-up procedure for the high-performance liquid chromatographic analysis of chloramphenicol in milk and eggs

Water, C. van de; Tebbal, D.; Haagsma, N.

doi:10.1016/0021-9673(89)90018-6

Cited by 30 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Folded filters (S&S 595 \) were from Schleicher and Schiill (Dassel, FRG). The monoclonal antibodies directed against CAP were prepared as described earlier (van de Water et al, 1987) and were immobilized as described earlier (van de Water et al, 1989). The immobilized monoclonal antibody preparation (i.e.…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

“…The sample was also analysed by a HPLC procedure described earlier (van de Water et al, 1989). The CAP content was found to be 6-7 /zg/kg.…”

Section: Milk Of a Chloramphenicol-treated Cowmentioning

confidence: 99%

“…Many chromatographic methods have been described for the determination of CAP in milk (Wai et al, 1979;Wai et al, 1980;Holtmannspotter & Thier, 1982;Be"cheiraz et al, 1983;Petz, 1983;van der Stroom-Kruyswijk, 1983;Malisch et al, 1984;Allen, 1985, Bergner-Lang & Kachele, 1985Malisch, 1987;van de Water et al, 1989). Apart from these methods, immunoassays have been described for this purpose.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in Milk

Water

Haagsma

1990

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

A sensitive streptavidin-biotin enzyme-linked immunosorbent assay (ELISA) for the detection of chloramphenicol (CAP) in milk is described. This test is based on a procedure developed earlier for the direct detection of CAP in crude aqueous meat extracts. Milk samples were defatted by centrifugation, filtered and directly submitted to the ELISA procedure. The results were compared with the values obtained by analysis of a part of the sample from which CAP was removed by an immobilized monoclonal antibody preparation. In spiked samples the presence of CAP at concentrations of 1-0 µg/kg and higher can be easily demonstrated. The possibility of the interpretation of the ELISA results with a statistical method, i.e. the so-called standard deviation ratio (SDR) procedure, was also investigated.

show abstract

Section: Reagents and Chemicalsmentioning

confidence: 99%

“…The sample was also analysed by a HPLC procedure described earlier (van de Water et al, 1989). The CAP content was found to be 6-7 /zg/kg.…”

Section: Milk Of a Chloramphenicol-treated Cowmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in Milk

Water

Haagsma

1990

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several authors have reported the use of enzymic digestion with (3-glucuronidase prior to extraction in order to liberate conjugated chloramphenicol (Keukens et al 1986, Nouws et al 1986, Van de Water and Haagsma 1993. There is evidence from various studies that chloramphenicol may be protein-bound in cattle plasma, milk, egg and trout muscle (Nouws et al 1986, Van de Water et al 1989, Baradat et al 1993. Attempts to liberate protein-bound chloramphenicol from human tissues by protease digestion were reportedly unsuccessful due to on-going metabolism during sample preparation (Ashton 1989).…”

Section: Introductionmentioning

confidence: 99%

Aspects of extraction, spiking and distribution in the determination of incurred residues of chloramphenicol in animal tissues

Cooper

Tarbin

Farrington

et al. 1998

Food Additives and Contaminants

View full text Add to dashboard Cite

Studies of distribution, extraction procedures and spiking protocols in the determination of incurred chloramphenicol residues in animal tissues have been carried out. An extraction procedure involving glucuronidase enzyme digestion was found to extract 10 times more incurred chloramphenicol from pig kidney than direct extraction without digestion. However, neither protease digestion nor ultrasonic probe treatment resulted in improved chloramphenicol extraction. Chloramphenicol was found to be inhomogeneously distributed within kidney from a treated pig. Highest concentrations were detected in the renal medulla. Muscle tissues from the same animal were found to contain a lower concentration of chloramphenicol residues, but no chloramphenicol residues were detectable in the liver. Chloramphenicol recovery from spiked pig liver was found to be lower than that from kidney, but was improved by the addition of piperonyl butoxide before extraction. This additive had no effect on recovery from spiked pig or cattle kidney. The implications of these results for regulatory surveillance of animal tissue for chloramphenicol residues are discussed.

show abstract

“…A very simple and practical technique for sample purification and concentration from complex matrices is the use of immobilized antibodies as an immunoabsorber [24][25][26]. In the method described in this paper antibodies against M were immobilized by coupling to carbonyldiimidazole-activated trisacrylgel to create an immunoabsorber for sample purification.…”

Section: Introductionmentioning

confidence: 99%

Antibody-mediated clean-up of blood for simultaneous HPLC determination of morphine and morphine glucuronides

Beike

Köhler

Blaschke

1997

International Journal of Legal Medicine

View full text Add to dashboard Cite

For the interpretation of the concentration of morphine in blood samples of heroin consumers information about the concentration of the analgesic active morphine metabolite morphine-6-glucuronide is very important. Thus a simple but specific clean-up procedure based on immuno-affinity chromatography is presented for the extraction of morphine, morphine-3-glucuronide and morphine-6-glucuronide from whole blood in cases of fatal heroin overdose. The preparation of the immunoabsorber by immobilization of antibodies against morphine-3-BSA and morphine-6-KLH with carbonyldiimidazole-activated trisacrylgel is described. The separation of the extracts is achieved by HPLC using native fluorescence detection. The limits of detection for this method are 10ng for morphine and morphine glucuronides/g blood. The results for the concentration of morphine and morphine glucuronides in blood from seven cases of heroin overdose are presented. By calculating the quotients for the concentrations of morphine-6-glucuronide/morphine the time elapsed since the last intake of heroin is estimated.

show abstract

Monoclonal antibody-mediated clean-up procedure for the high-performance liquid chromatographic analysis of chloramphenicol in milk and eggs

Cited by 30 publications

References 8 publications

A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in Milk

A Sensitive Streptavidin‐Biotin Enzyme‐linked Immunosorbent Assay for Rapid Screening of Residues of Chloramphenicol in Milk

Aspects of extraction, spiking and distribution in the determination of incurred residues of chloramphenicol in animal tissues

Antibody-mediated clean-up of blood for simultaneous HPLC determination of morphine and morphine glucuronides

Contact Info

Product

Resources

About