Monoclonal rat antibodies to murine IgM determinants

Kincade, Paul W.; Lee, Grace; Sun, Leslie; Watanabe, Tsuguo

doi:10.1016/0022-1759(81)90220-9

Cited by 91 publications

(38 citation statements)

References 16 publications

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“…A variant of the common leukocyte-specific antigen CD45R is expressed predominantly in B-cells and a subset of macrophages (35) and is recognized by the B220 antibody. An antibody against mouse IgM served as isotype-matched control (36). The results of these analyses are shown in Fig.…”

Section: Characterization Of In Vitro Differentiated Myeloid Cellsmacmentioning

confidence: 99%

Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice

Jägle

Müller

Kohler

et al. 1997

Journal of Biological Chemistry

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Section: Characterization Of In Vitro Differentiated Myeloid Cellsmacmentioning

confidence: 99%

Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice

Jägle

Müller

Kohler

et al. 1997

Journal of Biological Chemistry

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“…The anti-mouse IgD hybridoma 1.19 was provided by G. Klaus (NIMR, Mill Hill, U.K.). Anti-mouse Fc␥RIIb mAb (2.4G2) (28), anti-(187.1) (29), anti-mouse CD3 (2C11) (30), anti-mouse IgE (R1E4) (31), anti-mouse IgM (RS3.1) (32), and two anti-mouse IgM mAbs 331.12 (33) and Bet-2 (34) were purified from hybridoma supernatant by protein G affinity chromatography (Pharmacia-LKB, Uppsala, Sweden). Unlabeled and biotin-conjugated goat anti-mouse IgG1 Abs were purchased from Southern Biotechnology Associates (Birmingham, AL).…”

Section: Reagents and Absmentioning

confidence: 99%

Cross-Linking Surface Ig Delays CD40 Ligand- and IL-4-Induced B Cell Ig Class Switching and Reveals Evidence for Independent Regulation of B Cell Proliferation and Differentiation

Rush

Hasbold

Hodgkin

2002

The Journal of Immunology

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T cells stimulate B cells to divide and differentiate by providing activating signals in the form of inducible membrane-bound molecules and secreted cytokines. Provision of these signals in vitro reproduces many of the consequences of T-B collaboration in the absence of any form of Ag stimulation. Although clearly not obligatory, Ag signals appear to play an important regulatory role in numerous aspects of the B cell response. To examine directly the effect of an Ag signal, naive B cells were stimulated in the presence of rCD40 ligand, with or without IL-4 in the presence or absence of different anti-Ig mAbs. Anti-Ig mAbs exerted variable effects on the B cell division rate, from enhancement to no effect to inhibition. In contrast, all anti-Ig mAbs tested inhibited division-linked isotype switching to IgG1 and IgE. Thus, B cell Ag receptor ligands could modify the rates of B cell expansion and class switching independently. The ability of anti-Ig reagents to modify class switching suggests the B cell Ag receptor may play an important role in the selection of Ig isotypes during T cell-dependent humoral immune responses to Ags of different physical structure.

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“…Supernatant culture medium from the TSA-IL7 cell line (22) was used at a concentration of 5% as a source of recombinant mouse IL-7 to maintain progenitor B-cell cultures. Monoclonal rat antimouse antibodies against heavy-chain [331 (23)], CD43 [S7 (24)], and Mac-1 [M1͞70 (25)] were kindly provided by Alan Stall (Columbia University). Avidin-Texas red and antibodies against B220 [RA3-6B2 (26)] were purchased from PharMingen.…”

Section: Methodsmentioning

confidence: 99%

Increased sensitivity to apoptotic stimuli in c-abl-deficient progenitor B-cell lines

Dorsch

Goff

1996

Proc. Natl. Acad. Sci. U.S.A.

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The protooncogene c-abl encodes a nonreceptor tyrosine kinase whose cellular function is unknown. To study the possible involvement of c-Abl in proliferation, differentiation, and cell cycle regulation of early B cells, long-term lymphoid bone marrow cultures were established from c-abl-deficient mice and their wild-type littermates. Interleukin 7-dependent progenitor B-cell clones and lines expressing B220 and CD43 could be generated from both mutant and wild-type mice. The mutant and wild-type lines displayed no difference in their proliferative capacity as measured by thymidine incorporation in response to various concentrations of interleukin 7. Similarly, c-abl deficiency did not interfere with the ability of mutant clones to differentiate into surface IgM-positive cells in vitro. Analysis of cultures after growth factor deprivation, however, revealed a strikingly accelerated rate of cell death in c-abl mutant cells, due to apoptosis as confirmed by terminal deoxynucleotidyltransferase-mediated UTP nick end labeling analysis. Furthermore, a greater susceptibility to apoptotic cell death in c-abl mutant cells was also observed after glucocorticoid treatment. These results suggest that mutant c-Abl renders the B-cell progenitors more sensitive to apoptosis, and may account for some of the phenotypes observed in c-abl-deficient animals.

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Monoclonal rat antibodies to murine IgM determinants

Cited by 91 publications

References 16 publications

Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice

Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice

Cross-Linking Surface Ig Delays CD40 Ligand- and IL-4-Induced B Cell Ig Class Switching and Reveals Evidence for Independent Regulation of B Cell Proliferation and Differentiation

Increased sensitivity to apoptotic stimuli in c-abl-deficient progenitor B-cell lines

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