Monolayers on disordered substrates: self-assembly of alkyltrichlorosilanes on surface-modified polyethylene and poly(dimethylsiloxane)

Ferguson, Gregory S.; Chaudhury, Manoj K.; Biebuyck, Hans A.; Whitesides, George M.

doi:10.1021/ma00074a007

Cited by 152 publications

(151 citation statements)

References 3 publications

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“…Although organic polymers have a poorly characterized surface composition, they are also amenable to SAM derivatization after certain chemical treatments. 45,[50][51][52][53][54][55][56] Despite issues of monolayer order and biointeraction, the use of SAMs in biopatterning is appealing because the adhesiveness of the surface is engineered at a molecular level, 57 and at the same time biopatterning is reduced to patterning the SAM. 58 …”

Section: Iiia Engineering Cell-adhesiveness Of a Substratementioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Iiia Engineering Cell-adhesiveness Of a Substratementioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…Two layers of approximately 200 µm thickness were spun and baked before exposure and development. Before use, the structures were silanized using 1H,1H,2H,2H-perfluorooctyl trichloro silane (Sigma) as previously described 18 . The wafers were spin coated with PDMS (RTV615, General electric, 60 s at 150 rpm and 120 s at 350 rpm).…”

Section: Methodsmentioning

confidence: 99%

Live mammalian cell arrays

et al. 2013

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High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We have developed a simple, yet powerful method that uses contact spotting to generate highdensity nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries. High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We have developed a simple, yet powerful method that uses contact spotting to generate highdensity nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries.Cell-based assays and the tools used to perform them are constantly undergoing improvements toward higher experimental throughput, reduced reagent consumption, and advanced control of the cell microenvironment 1,2 . There are currently two main approaches for conducting highcontent cell culture experiments. In the first, cells are cultured in microtiter plates and robotic equipment is used to perform all necessary fluidic operations 3 . Although effective, this method remains unavailable to many laboratories due to the requirement for expensive robotics. A second approach that can be used for high-throughput cell studies is reverse transfection [4][5][6] . Here, cells are seeded onto an array of DNA, RNA, or small molecules. Large gene expression and silencing studies can be conducted in this manner, but complex cell libraries cannot be investigated since each array is limited to a single cell type.As an alternative to these approaches, contact spotting offers an affordable yet high-throughput platform. This technique is extensively used to generate high-density DNA and protein arrays and has been adapted to a variety of other purposes. For example, Hart et al. created a high-content immunoassay by spotting fixed mammalian cells that were cultured under different conditions 7 . To date, contact spotting has not been used to array live mammalian cells due to rapid spot evaporation and consequent cell death. Therefore, arraying of live mammalian cells has been limited to inkjet printing, which lacks the ability to handle a large number of different samples 8,9 .Large collections of mammalian cell lines have recently become available, including primary tumor and cancer cell lines 10 , stably transfected expression cell lines, and GFP-fusion libraries 11 . Novel approaches are required to efficiently assemble complex arrays of hundreds to thousands of genetically diverse cells. To address this arising need, we developed a simple, fast, and scalable method that uses standard microarray printing tools to generate high-density nanowell arrays. A minimal sample requirement of 500 cells enables the interrogation of cells that are availab...

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“…Traditional transformation of a hydrophobic polymer such as poly(dimethylsiloxane) (PDMS) to a hydrophilic state has been achieved via techniques such as corona and plasma treatments (Ferguson et al, 1993;Owen, 2005). Corona, plasma, irradiation, and laser technologies are ideal for textile surface modifications due to the energy-efficient dry-state processing, continuous on-line applicability, and minimal precursor quantity requirements.…”

Section: Physical Methodsmentioning

confidence: 99%