Monoliths for the purification of whey protein–dextran conjugates

Etzel, Mark R.; Bund, Tejashree

doi:10.1016/j.chroma.2011.01.025

Cited by 13 publications

(3 citation statements)

References 28 publications

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“…In the last 30 years, rigid porous monoliths have also been introduced and developed. The single solid continuous matrix has no interstitial voids and can also vastly improve productivity by operating at much higher flowrates than packed-bed chromatography [5] . Current advantages in industry have been realised in the polishing stage of monoclonal antibody purification using flowthrough mode where a membrane column binds impurities and allows the target to pass through [6] .…”

Section: Introductionmentioning

confidence: 99%

Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography

Dods

Hardick

Stevens

et al. 2015

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Fabricating electrospun cellulose nanofibre adsorbents for ion-exchange chromatography

Dods

Hardick

Stevens

et al. 2015

Journal of Chromatography A

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“…Moving away from beaded adsorbents to supports allowing convective flow reduces the mass transfer and pressure drop limitations (Jungbauer, 2005). Monolith structures with derivatized porous channels allow for bioseparation while employing convective mass transfer (Xie et al, 2002) enabling operation at high flowrates (Etzel and Bund, 2011; Peters et al, 1997). To date commercial applications have focused on large molecules such as plasmids where capacities are optimal (Urthaler et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Nanofiber adsorbents for high productivity downstream processing

Hardick

Dods

Stevens

et al. 2012

Biotech & Bioengineering

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Electrospun polymeric nanofiber adsorbents offer an alternative ligand support surface for bioseparations. Their non-woven fiber structure with diameters in the sub-micron range creates a remarkably high surface area. To improve the purification productivity of biological molecules by chromatography, cellulose nanofiber adsorbents were fabricated and assembled into a cartridge and filter holder format with a volume of 0.15 mL, a bed height of 0.3 mm and diameter of 25 mm. The present study investigated the performance of diethylaminoethyl (DEAE) derivatized regenerated cellulose nanofiber adsorbents based on criteria including mass transfer and flow properties, binding capacity, and fouling effects. Our results show that nanofibers offer higher flow and mass transfer properties. The non-optimized DEAE-nanofiber adsorbents indicate a binding capacity of 10% that of packed bed systems with BSA as a single component system. However, they operate reproducibly at flowrates of a hundred times that of packed beds, resulting in a potential productivity increase of 10-fold. Lifetime studies showed that this novel adsorbent material operated reproducibly with complex feed material (centrifuged and 0.45 µm filtered yeast homogenate) and harsh cleaning-in-place conditions over multiple cycles. DEAE nanofibers showed superior operating performance in permeability and fouling over conventional adsorbents indicating their potential for bioseparation applications.

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“…Using this affinity support, LF was purified from human skimmed milk with purity higher than 95%, in one step. In another application, Etzel and Bund [27] purified whey protein-dextran conjugates from a feed solution also containing un-reacted protein and dextran using either a cation exchange packed bed column or a tube monolith. Binding capacities were similar for both monolith and beaded column (4-6 mgmL -1 ).…”

mentioning

confidence: 98%