Monosaccharide composition analysis of immunomodulatory polysaccharides by on‐line hollow fiber microextraction with high‐performance liquid chromatography

Wang, Nani; Wang, Xuping; Huang, Xiaowen; Mao, Zhu-Jun; Zhang, Yang; Yu, Yong; Shou, Dan

doi:10.1002/jssc.201501205

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Monosaccharide identification was achieved by HPLC analysis (Wang et al., ). The samples were hydrolyzed with trifluoroacetic acid (TFA), and then the released monosaccharides were pre‐column derivatization with 1‐phenyl‐3‐methy‐5‐pyrazolone (PMP).…”

Section: Methodsmentioning

confidence: 99%

Microwave‐Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity

Zhang

Cai

Tian

et al. 2019

Journal of Food Science

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Four polysaccharide fractions (P‐1: 71.40%, P‐2: 1.95%, P‐3: 1.14%, P‐4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose‐52 anion‐exchange chromatography. Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet–visible detector. The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability). The highest content fraction P‐1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P‐4 > P‐3 > P‐2 > PSP > P‐1. After processed by microwave‐assisted degradation, the molecular weight of P‐1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P‐1 was about eightfold higher than natural P‐1. These results indicated that the proposed microwave‐assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides. Practical Application This study provided an environmentally friendly, convenient and efficient microwave‐assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum. The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.

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Section: Methodsmentioning

confidence: 99%

Microwave‐Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity

Zhang

Cai

Tian

et al. 2019

Journal of Food Science

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“…The PMP derivatization of monosaccharides was carried out according to previous method [27,55,56,57] with minor modifications. The resulting monosaccharides were dissolved in 5 mL ammonia (28%).…”

Section: Methodsmentioning

confidence: 99%

Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

et al. 2019

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Polyporus umbellatus is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization–ion trap–time of flight–mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10–103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.

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“…An RP HPLC with spectrophotometric detection techniques is often associated with the separation of ASs, but due to lack of chromophore in their structure and insufficient separation from other polar substances, pre‐ or postcolumn derivatization methods for selective and reproducible chromatographic separations have been employed. Anthranilic acid , 1‐phenyl‐3‐methyl‐5‐pyrazolone , phenyl isothiocyanate (PITC) , 9‐fluorenylmethoxycarbonyl salts (FMOC) , 1‐naphthyl isothiocyanate , o ‐phthalaldehyde (OPA) , or OPA/3‐mercaptopropionic acid have been reported as derivatization reagents for ASs. However, several problems are often related with most of the derivatization reagents, including elimination of excess reagent after reaction, stability of degradation products, or formation of dimers.…”

Section: Introductionmentioning

confidence: 99%

Development of a precolumn derivatization HPLC method with diode‐array detection for the determination of amino sugars in peat and soil humic acids

Beňo

Góra

Hutta

2018

J of Separation Science

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The work is focused on the development of a high-performance liquid chromatography method with diode-array detection for the separation and quantitation of the three most abundant amino sugars; d-glucosamine, d-galactosamine, and d-mannosamine. The high-performance liquid chromatography separation was carried out by reversed-phase chromatography on Chromolith Performance RP-18e monolithic column after acid hydrolysis (5 M HCl) and precolumn derivatization of samples using diethyl ethoxymethylenemalonate. Gradient elution and a mobile phase composed of ammonium formate buffer solution (10 mmol/L, pH 3.60) and methanol with flow rate of 1.0 mL/min were used. The monitoring wavelength was set at 280 nm. The limits of detection and quantitation for analytes ranged from 0.017 to 0.122 mg/L and from 0.057 to 0.407 mg/L, respectively. The proposed method was successfully applied for the determination of amino sugars in samples of humic acids isolated from different soils and peat.

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Monosaccharide composition analysis of immunomodulatory polysaccharides by on‐line hollow fiber microextraction with high‐performance liquid chromatography

Cited by 8 publications

References 24 publications

Microwave‐Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity

Microwave‐Assisted Degradation of Polysaccharide from Polygonatum sibiricum and Antioxidant Activity

Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

Development of a precolumn derivatization HPLC method with diode‐array detection for the determination of amino sugars in peat and soil humic acids

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