Monospecific high-affinity and complement activating anti-GM1 antibodies are determinants in experimental axonal neuropathy

Notturno, Francesca; Boccio, Piero Del; Luciani, Mirella; Caporale, Christina M.; Pieragostino, Damiana; Prencipe, Vincenza; Sacchetta, Paolo; Uncini, Antonino

doi:10.1016/j.jns.2010.03.003

Cited by 4 publications

(7 citation statements)

References 41 publications

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“…Furthermore, in their study, the authors immunized mice lacking the enzymes necessary to develop complex gangliosides with lipo-oligosaccharides derived from C. jejuni and found that the mice developed monoclonal antibodies against GM1. The specificity and purity of the lipooligosaccharides is critical for the proper induction of the model (Notturno et al, 2010). When two lipo-oligosaccharides were derived from the same strain of C. jejuni, only the purer of the two induced the symptoms.…”

Section: Role Of Gangliosides In Aman Amsan and Animal Modelsmentioning

confidence: 99%

The Neuroimmunology of Guillain-Barré Syndrome and the Potential Role of an Aging Immune System

Hagen

Ousman

2021

Front. Aging Neurosci.

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Guillain-Barré syndrome (GBS) is a paralyzing autoimmune condition affecting the peripheral nervous system (PNS). Within GBS there are several variants affecting different aspects of the peripheral nerve. In general, there appears to be a role for T cells, macrophages, B cells, and complement in initiating and perpetuating attacks on gangliosides of Schwann cells and axons. Of note, GBS has an increased prevalence and severity with increasing age. In addition, there are alterations in immune cell functioning that may play a role in differences in GBS with age alongside general age-related declines in reparative processes (e.g., delayed de-differentiation of Schwann cells and decline in phagocytic ability of macrophages). The present review will explore the immune response in GBS as well as in animal models of several variants of the disorder. In addition, the potential involvement of an aging immune system in contributing to the increased prevalence and severity of GBS with age will be theorized.

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Section: Role Of Gangliosides In Aman Amsan and Animal Modelsmentioning

confidence: 99%

The Neuroimmunology of Guillain-Barré Syndrome and the Potential Role of an Aging Immune System

Hagen

Ousman

2021

Front. Aging Neurosci.

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“…In fact, strong complement activation by anti‐GM1 antibodies was related to the development of axonal neuropathy in C . jejuni LOS‐sensitized rabbits 54 . Although the degree of complement activation by anti‐GQ1b antibodies was not associated with disease extension in atni‐GQ1b antibody syndrome, further studies are required to evaluate the clinical significance of diverse levels of complement activation in anti‐GM1‐positive GBS 55 …”

Section: Complement Activationmentioning

confidence: 99%

“…High affinity is the result of a mature immune response, probably through the selection of high‐affinity clones that produce the antibodies. The relative affinity of IgG anti‐GM1 antibodies has been measured using the chaotropic agent (sodium thiocyanate) elusion technique and the soluble antigen binding inhibition technique, and the affinity against GM1 differs between individual antibodies 54,57,58 …”

Section: Affinitymentioning

confidence: 99%

“…In an experiment where rabbits were immunized by LOS from a C . jejuni strain carrying the GM1 epitope, rabbits with high‐affinity anti‐GM1 antibodies manifested axonal neuropathy, whereas those with low‐affinity antibodies did not 54 . Investigation of serial changes in antibody affinity in GM1‐immunized rabbits showed that antibody affinity was higher at the disease onset compared with 1 or 2 weeks before onset, although antibody titers and fine specificity remained unchanged 60 .…”

Section: Affinitymentioning

confidence: 99%

“…The relative affinity of IgG anti-GM1 antibodies has been measured using the chaotropic agent (sodium thiocyanate) elusion technique and the soluble antigen binding inhibition technique, and the affinity against GM1 differs between individual antibodies. 54,57,58 Several studies have reported the association of the affinity of anti-GM1 antibodies with the development of GBS in patients and animal models. Axonal degeneration occurred significantly more frequently in patients with high-affinity anti-GM1 antibodies than in those with low-affinity antibodies, 57 whereas another group reported that disease severity was not associated with the affinity of anti-GM1 antibodies.…”

Section: Affinit Ymentioning

confidence: 99%

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Multifaceted features of immunoglobulin G anti‐GM1 antibodies in Guillain–Barré syndrome

Koga

2021

Clinical & Exp Neuroim

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Immunoglobulin G (IgG) anti‐GM1 antibodies, the most common autoantibodies in Guillain–Barré syndrome (GBS), have diverse characteristics regarding IgG subclass, complement activation, affinity and fine specificity. In this review, an update on multifaceted aspects of IgG anti‐GM1 antibodies is provided, emphasizing their roles in creating a varying clinical picture of GBS and in developing pure motor neuropathy after antecedent infection. Diversity in IgG subclass and cross‐reactivity, both of which are closely related to the type of antecedent infection, appears to cause the variation in clinical features and severity of the disease. The environment around GM1 expression in neural tissue is thought to interfere with the binding of anti‐GM1 antibodies by modulating the immunoreactive epitope, allowing only anti‐GM1 antibodies with specific fine specificity to bind to the GM1 ganglioside. This might create the characteristic clinical picture of pure motor neuropathy in anti‐GM1‐positive GBS, although GM1 is present equally in motor and sensory nerves. The environment around the GM1 epitope on Campylobacter jejuni, the most frequently identified agent in antecedent infections of GBS, might also be important in creating diversity in the fine specificity of anti‐GM1 antibodies, possibly preventing the development of GBS in most patients with enteritis by the GM1 epitope‐bearing C. jejuni. Because there remains no direct evidence indicative that diversity of anti‐GM1 antibodies is involved in the development of GBS, further study is required.

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Molecularly imprinted and cladded nanoparticles for high-affinity recognition of structurally closed gangliosides

2022

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Monospecific high-affinity and complement activating anti-GM1 antibodies are determinants in experimental axonal neuropathy

Cited by 4 publications

References 41 publications

The Neuroimmunology of Guillain-Barré Syndrome and the Potential Role of an Aging Immune System

The Neuroimmunology of Guillain-Barré Syndrome and the Potential Role of an Aging Immune System

Multifaceted features of immunoglobulin G anti‐GM1 antibodies in Guillain–Barré syndrome

Molecularly imprinted and cladded nanoparticles for high-affinity recognition of structurally closed gangliosides

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