No 60 Ni or 61 Ni anomalies have been detected in troilite inclusions from Muonionalusta, a 4565.3 ± 0.1 Ma old IVA iron meteorite, to the level of the analytical precision of 10 ppm. Because Muonionalusta troilite is very old, has a high Fe/Ni ratio, and is free of 61 Ni anomalies, it is the ideal material in which to search for potential excesses of 60 Ni produced by the decay of 60 Fe (t 1/2 = 2.62 Ma). The 60 Ni/ 58 Ni and Fe/Ni ratios (up to 1680) measured here imply an upper limit for the initial 60 Fe/ 56 Fe for the Muonionalusta troilite of 3 × 10 −9 . Assuming that 60 Fe was homogeneously distributed across the nebula, this result suggests that the solar system initial 60 Fe/ 56 Fe ratio was less than 5 × 10 −9 , which is lower than previously measured by at least a factor of 50.