More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer

Abstract

Based on the ability of the probiotics in the gut microflora modification, they can have the beneficial effects on diseases in the short and/or the long term. In previous study, we revealed that unlike Bifidobacterium bifidum, the amount of Lactobacillus acidophilus remained almost unchanged in mice gut microflora in the long term, indicating more stability of L. acidophilus than B. bifidum which can be used to prevent some incurable diseases such as cancer. Thirty-eight male BALB/c mice were divided into four groups, control, azoxymethane (AOM), L. acidophilus, and B. bifidum probiotics, to evaluate the protective effects of the probiotics on AOM-induced mouse colon cancer. Except for the control group, the rest of the animals were weekly given AOM (15 mg/kg, s.c) in three consecutive weeks. Colon lesion incidence was 74% in the AOM group in comparison with the control (0%) (P < 0.05). The lesions were varied from mild to severe dysplasia and colonic adenocarcinoma. Administration of the probiotics inhibited the incidence of colonic lesions by about 57% in L. acidophilus (P < 0.05) and 27% in B. bifidum (P > 0.05) compared to the AOM group. The serum levels of CEA and CA19-9 tumor markers were significantly decreased in L. acidophilus in comparison with the AOM group (P < 0.05). Moreover, the serum levels of IFN-γ and IL-10 and the number of CD4 and CD8 cells were significantly increased in L. acidophilus compared to AOM (P < 0.05). Our study highlighted the more potential effects of L. acidophilus probiotic than B. bifidum on mouse colon cancer.

Combination of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis Shows a Stronger Anti-Inflammatory Effect than Individual Strains in HT-29 Cells

“…Due to this stability, L. acidophilus has been considered an ideal vehicle for mucosal-targeted delivery of drugs [24]. The amount of L. acidophilus remained almost unchanged in the gut for a long time [25].…”

Interconnections between the Oral and Gut Microbiomes: Reversal of Microbial Dysbiosis and the Balance between Systemic Health and Disease

“…Potential risk reduction in CRC following synbiotic supplementation was suggested in a study of oligofructose-enriched inulin, Lactobacillus, and Bifidobacterium in which several CRC biomarkers, including CEA and CA19-9 tumor markers, improved and significant changes in fecal flora were produced, including increased Bifidobacterium and Lactobacillus populations and decreased Clostridium perfringens [83,84]. Additionally, treatment of CRC with supplementation of inulin-type fructans was shown to inhibit growth and induce apoptosis in colon tumor cells [85].…”

Modulation of Anti-Tumour Immune Responses by Probiotic Bacteria

“…L. casei ATCC 393 also delayed the onset of cancer, reduced the number of aberrant crypt foci [ 97 ], and modified pro-inflammatory cytokine levels in serum and T cell populations in the spleen [ 98 ] in DMH-induced colon carcinogenesis in mice. In addition, in a mouse model of azoxymethane (AOM) carcinogenesis, L. acidophilus reduced colonic lesions by 57%, and increased serum levels of IFN-γ and IL-10, as well as the number of circulating CD4+ and CD8+ T cells [ 99 ]. Another LAB strain, Pediococcus pentosaceus GS4, tested in an AOM mouse model, mitigated colon cancer, inducing apoptosis in malignant colonocytes and a lower histone deacetylase (HDAC) activity [ 100 ].…”