Bicycling is a sustainable form of micromobility and offers numerous health and environmental benefits. Scientific studies investigating bikeability have grown substantially, especially over the past decade. This paper presents a systematic literature review of the developed urban bikeability indices (BIs). The paper provides insight into the scientific literature on bikeability as a tool to measure bicycle environment friendliness; more importantly, the paper seeks to know if the BIs consider bicycle infrastructure design principles. Data extraction included identifying the geographical location, essential indicators, sample size and distribution, data source, the unit of analysis, measurement scale, methods used to weigh indicators, and identification of studies using bicycle design principles in BIs. The database search yielded 1649 research articles using different keywords and combinations, while 15 studies satisfied the inclusion criteria. The studies were found to be conducted in various geographical locations. The unit of analysis for developing the index varied across studies, from street segments or bicycle lanes to zones within the city or even the entire city. The most commonly utilized method in developing urban BIs was a scoring and weighting system to weigh the indicators. The weighting methods include an equal weight system, survey-based and literature review-based methods, expert surveys, the analytic hierarchy process, and a weighted linear combination model. The essential criterion is bicycle infrastructure, such as bike lanes, routes, and bicycle paths as 14 studies considered it for the construction of the BIs. The review findings suggest a lack of consideration of all five bicycle infrastructure design principles, as only three studies considered them all, while others only included a subset. Safety and comfort are the most commonly considered principles, while coherence is the least considered principles in the BIs. It is crucial to consider all five bicycle infrastructure design principles to create a bicycle-friendly environment and attract more people to this sustainable mode of transportation.