Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa

Ardina, Rinny; Rosalinda, Sherly

doi:10.33084/jsm.v3i2.91

Cited by 10 publications

(12 citation statements)

References 1 publication

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“…Leukosit dibagi menjadi lima jenis tipe yaitu basofil, eosinofil, neutrofil, limfosit dan monosit [1]. Pendeteksian jenis sel darah putih selama ini dilakukan di laboratorium dengan melakukan preparat darah yang sudah dilakukan pewarnaan terlebih dahulu sehingga pada mikroskop akan terlihat jelas dan kontras antara sel darah merah dan sel darah putih [2]. Hal ini menyebabkan dibutuhkan lebih usaha, waktu, dan biaya dalam mendeteksi jenis sel darah putih, terutama jika preparat yang diperlukan berjumlah banyak.…”

Section: Pendahuluanunclassified

Pemanfaatan Scale Invariant Feature Transform Berbasis Saliency untuk Klasifikasi Sel Darah Putih

Yohannes

Devella

Hadisaputra

2021

JuTISI

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White blood cells are cells that makeup blood components that function to fight various diseases from the body (immune system). White blood cells are divided into five types, namely basophils, eosinophils, neutrophils, lymphocytes, and monocytes. Detection of white blood cell types is done in a laboratory which requires more effort and time. One solution that can be done is to use machine learning such as Support Vector Machine (SVM) with Scale Invariant Feature Transform (SIFT) feature extraction. This study uses a dataset of white blood cell images that previously carried out a pre-processing stage consisting of cropping, resizing, and saliency. The saliency method can take a significant part in image data and. The SIFT feature extraction method can provide the location of the keypoint points that SVM can use in studying and recognizing white blood cell objects. The use of region-contrast saliency with kernel radial basis function (RBF) yields the best accuracy, precision, and recall results. Based on the test results obtained in this study, saliency can improve the accuracy, precision, and recall of SVM on the white blood cell image dataset compared to without saliency.

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Section: Pendahuluanunclassified

Pemanfaatan Scale Invariant Feature Transform Berbasis Saliency untuk Klasifikasi Sel Darah Putih

Yohannes

Devella

Hadisaputra

2021

JuTISI

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“…Blood smears were dipped into Wright solution for 2 minutes then rinsed with aquadest solution. Blood smears were dipped into 10% Giemsa solution for 15 minutes [35]. Vaginal smears and blood smears were rinsed by aquadest solution after staining.…”

Section: Vaginal Smears and Blood Smears Stainingmentioning

confidence: 99%

Vaginal Smear and Neutrophil Count as an Alternative Method for Estrous Phase in Female Tiger (Panthera tigris sumatrae, Pocock, 1929)

Kiranaputri¹,

Sjahfirdi²,

Tumbelaka³

2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Detection of the estrous cycle is essential for basic reproductive aspects in all animals. Both natural mating and assisted reproductive techniques require estrous detection for breeding programs which will assist to maintain the tiger population in captivity. This research aims to explore alternative methods for tiger conservation in the remote area. Vaginal smear and neutrophil count were used to determine the phase of estrous cycle, and it easily applied on the field. Vaginal smears and blood smears were collected twice within interval 6 months n a female tiger at Tambling Wildlife Nature Conservation Rescue Centre. While tiger was restrained mechanically, blood samples were collected from the coccygeal vein and the vaginal smears were collected intravaginal. All procedure followed the animal welfare protocol. Vaginal smears were stained by 10% Giemsa. Basal/parabasal, intermediate, and superficial/cornified cells were identified to determine the reproductive periods under the microscope. Blood smears were stained by Giemsa-Wright. Neutrophils were counted within 200 white blood cells count under the microscope. The result showed that female tiger was in the follicular phase and neutrophil count was 78 cells at the 1 st sample collection. The 2 nd sample collection resulted in luteal phase with 55 neutrophils count. This research suggests vaginal smear and neutrophil count can be used as an alternative method of tiger estrous phase detection. In addition, these methods have economical and practical value.

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“…After which, it was dipped into Wright's solution for 2 minutes. Blood smears were rinsed slowly using aquadest then dipped into 10% Giemsa solution for 15 minutes (Ardina and Roslinda 2018). After dipping in Giemsa solution, blood smears then rinsed slowly using aquadest.…”

Section: Blood Smears Stainingmentioning

confidence: 99%

Positive reinforcement conditioning as Sumatran tiger's (Panthera tigris sumatrae) social enrichment at Tambling Wildlife Nature Conservation Rescue Centre, Lampung, Indonesia

et al. 2021

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Abstract. Kiranaputri G, Sjahfirdi L, Tumbelaka LITA, Yana A, Priyanto SK, Anggarsari LY, Marizal. 2021. Positive reinforcement conditioning as Sumatran tiger's (Panthera tigris sumatrae) social enrichment at Tambling Wildlife Nature Conservation Rescue Centre, Lampung, Indonesia. Biodiversitas 23: 55-61. Tiger individuals are translocated to ex-situ conservations due to human-tiger conflicts and may express behavioral change (stereotypic) in captivity. Furthermore, medical check-up routines may cause injury and stress between tiger and operators under tough circumstances. Positive reinforcement conditioning (PRC) is a well-known method to minimize the risks on medical examination and as social enrichment. Therefore, the purposes of this research are (i) to examine PRC on tiger's blood sample collections and (ii) the correlation between physiological stress and tiger's stereotypic behavior (SB) through the neutrophil per lymphocyte ratio (N/L ratio) method. Four Sumatran tigers’ (1 female, 3 males) behavior were observed using focal animal sampling at Tambling Wildlife Nature Conservation Rescue Centre, Lampung, Indonesia. Each baseline and post-enrichment tiger's behavioral observations were conducted for 1.920 minutes (1 male & 1 female) and 960 minutes (2 males). Then SB was categorized into low (<33,33%), intermediate (33,34-66,66%) or high (>66,67%). Blood collections (BC) were conducted twice (1st without PRC and 2nd with PRC) directly on the tiger after the behavior observation. Tiger's physiological stress during BC was analyzed with Wilcoxon. The correlation between physiological stress and SB was analyzed with Kruskal-Wallis. All tigers’ N/L ratios were in normal value from this present research during both BC. The tigers did not show chronical stress as long this research was conducted. PRC was significantly effective to reduce tiger physiological stress during BC (Z= -0,730, P= 0,465 < 0,05). All tigers showed low (<33,33%) pacing SB (without fur-plucking, self-biting, and self-mutilation behavior) both in baseline and post-enrichment. Any fur-plucking, self-biting & self-mutilation behaviors were not observed. Physiological stress was not correlated to SB ( = 3, P= 0,392 > 0,05). The PRC was an appropriate and effective tool to handle Sumatran tigers during BC. These tigers performed SB as a coping mechanism in the enclosure.

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Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa

Cited by 10 publications

References 1 publication

Pemanfaatan Scale Invariant Feature Transform Berbasis Saliency untuk Klasifikasi Sel Darah Putih

Pemanfaatan Scale Invariant Feature Transform Berbasis Saliency untuk Klasifikasi Sel Darah Putih

Vaginal Smear and Neutrophil Count as an Alternative Method for Estrous Phase in Female Tiger (Panthera tigris sumatrae, Pocock, 1929)

Positive reinforcement conditioning as Sumatran tiger's (Panthera tigris sumatrae) social enrichment at Tambling Wildlife Nature Conservation Rescue Centre, Lampung, Indonesia

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