Morfologia do canal alimentar de lagartas de Spodoptera frugiperda (J E Smith) (Lepidoptera: Noctuidae) alimentadas com folhas tratadas com nim

Correia, Alicely Araújo; Wanderley‐Teixeira, Valéria; Teixeira, Álvaro Aguiar Coelho; Oliveira, José Vargas de; Torres, Juliana Rezende

doi:10.1590/s1519-566x2009000100008

Cited by 36 publications

(5 citation statements)

References 18 publications

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“…Vacuolization and changes in the brush border of the midgut digestive cells of the A. aegypti larvae may indicate that these cells were dying, perhaps owing to the toxicity of squamocin, as described in Culex quinquefasciatus (Diptera: Culicidae) subjected to synthetic insecticides [ 22 ] and Spodoptera frugiperda (Lepidoptera: Noctuidae) treated with neem extract [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Costa

Cossolin

Pereira

et al. 2014

Toxins

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Acetogenins are secondary metabolites exclusively produced by Annonaceae, which have antitumor, cytotoxic, and pesticide activities. In this study, we evaluated the larvicidal and cytotoxic effect of squamocin from Annona squamosa on Aedes aegypti (Diptera: Culicidae) midgut. The compound was solubilized in 2% Tween 20 at 10, 20, 50, 80 and 100 ppm. The assay was conducted in a completely randomized design with four replications, each with 20 third-instar larvae. Larval mortality was assessed every hour until total mortality, and the data were subjected to Probit analysis. Cellular damage was evaluated every 30 min in groups comprising five larvae subjected to squamocin at 50 and 100 ppm for 240 min. The total larval mortality occurred after 360 min following application of 50, 80, and 100 ppm squamocin, and 600 min after applying other concentrations with LC50 at 6.4 ppm. Both 50 and 100 ppm of squamocin showed cytotoxic activity in the midgut epithelium of A. aegypti after 240 min with 50 ppm resulting in midgut cells with light cytoplasm containing small vacuoles, whereas at 100 ppm were found cells with cytoplasm highly vacuolated, damaged apical surface and cell protrusion toward the gut lumen. In conclusion, squamocin has the potential to control A. aegypti.

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Section: Resultsmentioning

confidence: 99%

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Costa

Cossolin

Pereira

et al. 2014

Toxins

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“…Damaged cells can originate other faulty or imperfect cells (Barreto et al, 2006). Processes of reduction and damage in generative cells were described by Correia et al (2009) Spodoptera frugiperda caterpillars treated with neem.…”

Section: Discussionmentioning

confidence: 99%

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

et al. 2017

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The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days. The results showed alterations mainly regarding the damaged cell structures, such as yolk granules, organelles and the plasma membrane of the germ cells. In addition, structures related with defense mechanisms were found, such as vacuoles, cytoskeletal filaments, and myelin figures in the germ cells. Damages in the generative cells of the midgut, alterations in the size of digestive cells, the number of endosomes, digestive vacuoles, digestive residues, lipid drops and organelles in the cytoplasm of the digestive cells and the presence of microvilli in the plasma membrane of these cells also demonstrate the progressive damages caused by the action of dinotefuran in the midgut and germ cells of R. sanguineus semi-engorged females. The concentrations applied partially impaired the digestive processes; and, without proper nutrition, all the ectoparasite's physiologic events are prevented from occurring, leading the individual to death. The germ cells were also damaged, and probably would not be able to advance in their development (I-V) and complete the vitellogenesis, which would affect the fertility of the female and consequently impede the formation of a new individual.

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“…The digestive tract is divided into three regions: foregut, midgut, and hindgut. The midgut is the most extended region of the digestive tract and is the chief organ for digestion and absorption (Correia et al, 2009). Among the three areas of the digestive tract, a histological study was carried out on the anterior midgut region.…”

Section: Histological Studiesmentioning

confidence: 99%

Imidacloprid‐induced mortality, histopathology and biochemical impairments in the larvae of oriental latrine fly (Chrysomya megacephala)

Yasmeen

Amir

2023

Medical Vet Entomology

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The concentration–mortality response of third instar larvae of Chrysomya megacephala (Diptera: Calliphoridae) to a synthetic insecticide, imidacloprid, and its impact on histopathological, histochemical, and biochemical parameters were determined in laboratory assays. Larvae displayed a concentration and time‐dependent mortality response for the insecticide. Histopathological studies exhibited quite noticeable modifications in the epithelial cells, peritrophic membrane, basement membrane and muscular layer of the larval midgut. The ultrastructural analysis demonstrated alterations in nuclei, lipid spheres, microvilli, mitochondria, rough endoplasmic reticulum and lysosomes. In addition, histochemical tests on the midgut were performed, which revealed a strong reaction for proteins and carbohydrates in the control group and a weak reaction in the group exposed to imidacloprid in a dose and time‐dependent manner. Imidacloprid also caused a significant reduction in the total midgut content of carbohydrates, proteins, lipids and cholesterol. Larvae treated with imidacloprid also showed a reduction in the activities of acid and alkaline phosphatases at all concentrations compared to untreated larvae.

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Morfologia do canal alimentar de lagartas de Spodoptera frugiperda (J E Smith) (Lepidoptera: Noctuidae) alimentadas com folhas tratadas com nim

Cited by 36 publications

References 18 publications

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Larvicidal and Cytotoxic Potential of Squamocin on the Midgut of Aedes aegypti (Diptera: Culicidae)

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

Imidacloprid‐induced mortality, histopathology and biochemical impairments in the larvae of oriental latrine fly (Chrysomya megacephala)

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