Morin protects acute liver damage by carbon tetrachloride (CCl4) in rat

Abstract

The purpose of this study was to investigate possible beneficial effects of morin on CCl(4)-induced acute hepatotoxicity in rats. Rats received a single dose of CCl(4) (150 microL/100 g 1:1 in corn oil). Morin treatment (20 mg/kg) was given at 48, 24, and 2 h before CCl(4) administration. CCl(4) challenge elevated serum alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) levels, but these effects were prevented by the pretreatment of rats with morin. To identify the mechanism of protective activity of morin in CCl(4)-induced hepatotoxicity in rats, we investigated expressions of tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and inducible nitric oxide (iNOS). The expressions of TNF-alpha, IL-1beta, IL-6, and iNOS were increased by CCl(4) treatment and increased expressions of those were decreased by morin. These findings suggest that morin prevents acute liver damage by inhibiting the production of TNF-alpha, IL-1beta, IL-6, and iNOS.

Morin inhibits interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes

“…Their data were supported by Harasstani et al, who found the inhibition of NO production by morin in LPSinduced RAW 264.7 cells [20]. Similar results were also demonstrated by Lee and Aliev et al [30,31]. PGE 2 is also an inflammatory factor which is elevated by COX-2.…”

In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves

“…[14] Since the changes associated with CCl 4 -induced liver damage are similar to that of acute viral hepatitis,[15] CCl 4 -mediated hepatotoxicity was chosen as the experimental model. [1617] It has been established that CCl 4 is accumulated in hepatic parenchyma cells and metabolically activated by cytochrome P450-dependent monooxygenases to form a trichloromethyl radical (CCl 3 ). The CCl 3 radical alkylates cellular proteins and other macromolecules with simultaneous attack on polyunsaturated fatty acids, in the presence of oxygen, to produce lipid peroxides, leading to liver damage.…”

Saeng-Kankunbi-Tang (生肝健脾汤) protects liver against oxidative damage through activation of ERK/Nrf2 pathway

“…The largest lobe of the livers was sliced, and tissue slices were fixed in 10% neutral buffered formalin, then embedded in paraffin, sectioned 3-4 μm and stained with hematoxylin and eosin (HE) and Sirius red (SR) for general histopathology or with masson's trichrome (MT) for collagen fiber. (11,12) The histopathological profiles of each sample were observed under light microscope (Nikon, Japan).…”