Morphofunctional Changes in the Lung Vascular Endotheliocytes in Mice with Influenza A/H5N1 A/Goose/Krasnoozerskoye/627/05

Ковнер, А. В.; Потапова, О. В.; Shkurupy, V. A.

doi:10.1007/s10517-013-1981-z

Cited by 3 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the proteins with reduced expression identified in our study, the five most-reduced DEPs in samples from influenza-infected patients that were also correlated with higher viral loads were CTSD, KLK7, MFGE8, MAPK9 and CD27, respectively. Cathepsin D (CTSD) is a lysosomal aspartic protease that has been shown to be highly expressed in alveolar resident macrophages and Kupffer cells following IAV infections in mice [ 67 , 68 ]. KLK7 is a chymotrypsin-like serine protease that cleaves proteins at tyrosine, phenylalanine or leucine [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Respiratory Mucosal Proteome Quantification in Human Influenza Infections

et al. 2016

View full text Add to dashboard Cite

Respiratory influenza virus infections represent a serious threat to human health. Underlying medical conditions and genetic make-up predispose some influenza patients to more severe forms of disease. To date, only a few studies have been performed in patients to correlate a selected group of cytokines and chemokines with influenza infection. Therefore, we evaluated the potential of a novel multiplex micro-proteomics technology, SOMAscan, to quantify proteins in the respiratory mucosa of influenza A and B infected individuals. The analysis included but was not limited to quantification of cytokines and chemokines detected in previous studies. SOMAscan quantified more than 1,000 secreted proteins in small nasal wash volumes from infected and healthy individuals. Our results illustrate the utility of micro-proteomic technology for analysis of proteins in small volumes of respiratory mucosal samples. Furthermore, when we compared nasal wash samples from influenza-infected patients with viral load ≥ 28 and increased IL-6 and CXCL10 to healthy controls, we identified 162 differentially-expressed proteins between the two groups. This number greatly exceeds the number of DEPs identified in previous studies in human influenza patients. Most of the identified proteins were associated with the host immune response to infection, and changes in protein levels of 151 of the DEPs were significantly correlated with viral load. Most important, SOMAscan identified differentially expressed proteins heretofore not associated with respiratory influenza infection in humans. Our study is the first report for the use of SOMAscan to screen nasal secretions. It establishes a precedent for micro-proteomic quantification of proteins that reflect ongoing response to respiratory infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Respiratory Mucosal Proteome Quantification in Human Influenza Infections

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Hence, the necrotic processes in influenza are implemented via cytodestructive effects of the viruses; they are stimulated by hypoxia, ischemia, and thrombosis of the blood vessels resulting from enhanced tropicity of influenza viruses to endotheliocytes [4,9]. Seemingly, the apoptotic death of the cells is induced by hypoxia; its development depends on the number and activation degree of macrophages in the infiltrates and their up-regulated secretion of nitric oxide, proteases [1,2,4], and apoptosis-inducing factors, which is characteristic of mitochondrial pathway; moreover, apoptosis can be triggered from cell membrane via the plasmalemmal receptor pathway. Probably, the functional status and the number of activated macrophages can select the predominant pathways, which trigger apoptosis during viral influenzas.…”

Section: Resultsmentioning

confidence: 99%

“…In infected mice, immunohistochemistry visualized the positive staining of alveolocytes for viral antigen A [3][4][5].…”

Section: Resultsmentioning

confidence: 99%

“…Routine histological processing of the lung specimens included hematoxylin and eosin staining. Immunohistochemical methods [4] with specific primary antibodies were employed to detect the antigen of influenza A virus (InfA; Abcam) and expression of TNFα and TRAIL (DBS) as well as FAS and FADD (DBS) by the pulmonary cells. Expression of caspases 9 and 3 by alveolocytes was also assessed using Abcam assay kits.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Death Mechanisms of Pulmonary Alveolocytes in Mice Infected with Influenza Viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05

Черданцева

Ковнер

Sharkova³

et al. 2019

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

In CBA mice infected with influenza viruses A/H1N1/California/04/2009 and A/H5N1/Goose/ Krasnoozerskoye/627/05 in a dose of 10 MLD 50 , the mechanisms of death of pulmonary alveolocytes over 10 postinfection days were studied by light microscopy, immunohistochemistry, and morphometry. In mice infected with A/H1N1, alveolocytes died predominantly via necrosis, while apoptosis mostly employed the mitochondrial pathway. In mice infected with A/ H5N1, apoptosis was the dominant mechanism of alveolocyte death proceeded via membrane receptor signaling followed by switching to FAS-mediated pathway via activation of FADD, the apoptotic signal transduction protein.

show abstract

Studies of Influenza A/H1N1 A/Tomsk/13/2010 Virus Topology during Development of Infectious Process in Mammals

et al. 2016

View full text Add to dashboard Cite

Influenza A/H1N1 A/Tomsk/13/2010 virus registered in Siberia in 2010 proved to be an extremely pathogenic strain. Dynamic study of the topology of this influenza virus strain in the lungs, liver, kidneys, lymph nodes, and great vessels of infected mice was carried out. Influenza A virus was detected by immunohistochemical methods in cells of different histogenesis in all the studied organs throughout the observation period (days 1-30 postinfection), which indicated effective replication and long persistence of influenza A/H1N1 A/Tomsk/13/2010 virus in mammalian cells.

show abstract

Morphofunctional Changes in the Lung Vascular Endotheliocytes in Mice with Influenza A/H5N1 A/Goose/Krasnoozerskoye/627/05

Cited by 3 publications

References 7 publications

Respiratory Mucosal Proteome Quantification in Human Influenza Infections

Respiratory Mucosal Proteome Quantification in Human Influenza Infections

Death Mechanisms of Pulmonary Alveolocytes in Mice Infected with Influenza Viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05

Studies of Influenza A/H1N1 A/Tomsk/13/2010 Virus Topology during Development of Infectious Process in Mammals

Contact Info

Product

Resources

About