Morphogens, Compartments, and Pattern: Lessons from Drosophila?

Lawrence, Peter A.; Struhl, Gary

doi:10.1016/s0092-8674(00)81297-0

Cited by 537 publications

(348 citation statements)

References 96 publications

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“…The expression of conserved developmental regulators en and hh in TFCs during ovary morphogenesis may provide further insights into this process (Forbes et al, 1996b;Zhu and Xie, 2003). En is a transcription factor, while hh encodes a shortrange morphogen (Lawrence and Struhl, 1996). Both genes are highly conserved and play fundamental roles in pattern formation during Drosophila development (Lawrence and Struhl, 1996;Hidalgo, 1996).…”

Section: Discussionmentioning

confidence: 99%

Genetic dissection of a stem cell niche: The case of the Drosophila ovary

Bolı́var

Pearson

López-Onieva

et al. 2006

Developmental Dynamics

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In this work, we demonstrate a powerful new tool for the manipulation of the stromal component of a wellestablished Drosophila stem cell niche. We have generated a bric-a-brac 1 (bab1)-Gal4 line that drives UAS expression in many somatic ovary cell types from early larval stages. Using this Gal4 line, we could effectively induce FLP/FRT-mediated recombination in the stromal cells of the ovarian germline stem cell niche. Mutant clones were observed in the developing ovary of larvae and pupae, including in somatic cell types that do not divide in the adult, such as the cap cells and the terminal filament cells. Exploiting the ability of bab1-Gal4 to generate large clones, we demonstrate that bab1-Gal4 is an effective tool for analyzing stem cell niche morphogenesis and cyst formation in the germarium. We have identified a novel requirement for engrailed in the correct organization of the terminal filaments. We also demonstrate an involvement for integrins in cyst formation and follicle cell encapsulation. Finally using bab1-Gal4 in conjunction with the Gal80 system, we show that while ectopic dpp expression from stromal cells is sufficient to induce hyperplastic stem cell growth, neither activation nor inactivation of the BMP pathway within stromal cells affects germline stem cell maintenance.

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Section: Discussionmentioning

confidence: 99%

Genetic dissection of a stem cell niche: The case of the Drosophila ovary

Bolı́var

Pearson

López-Onieva

et al. 2006

Developmental Dynamics

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show abstract

“…Short range of action does not preclude morphogen activity. For example, a Wingless (Wnt) morphogen gradient is required for the fine-scale patterning of Drosophila segments (Lawrence and Struhl, 1996). Detection of such a gradient in Xenopus or zebrafish is simply beyond the resolution of analyses performed to date.…”

Section: Early Xenopus Morphogens In Vivo: Not Activin But Nodals?mentioning

confidence: 99%

“…The reader of this review is urged to refer to excellent accounts of some other morphogen systems, particularly in the Drosophila segmentation and imaginal disc patterning processes (Lawrence and Struhl, 1996;Day and Lawrence, 2000;Vervoort, 2000;Lawrence, 2001b) and in the dorsoventral patterning of the vertebrate neural tube (Lee and Jessell, 1999;Tabata, 2001;Marti and Bovolenta, 2002). However, there are perhaps two simplifying generalizations that can help in appreciating the complexity of morphogen-dependent patterning.…”

Section: Morphogens In Vivo Part Iii: Interacting Morphogensmentioning

confidence: 99%

“…A graded distribution of a morphogen provides both polarity (the slope of the gradient) and a scalar value (the concentration itself) that can be interpreted by cells according to threshold values to provide a coordinate system. The idea that morphogen gradients provide a coordinate system for guiding cell fate during embryonic development is over 100 years old (Wolpert, 1996;Lawrence and Struhl, 1996). It was first proposed by Thomas Hunt Morgan (later famous for pioneering Drosophila genetics) during his early studies on amphibian and planarian development and regeneration (quoted in Lawrence, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…As has emerged over the past 15 years of developmental biology, embryos of diverse species do in fact use morphogen gradients repeatedly in the development of the individual embryo. There have been several reviews that provide an overview of known morphogen gradients, especially in Drosophila development (Lawrence and Struhl, 1996;Dale, 1997;Strigini and Cohen, 1999;Tickle, 1999;Podos and Ferguson, 1999;Day and Lawrence, 2000;Tabata, 2001;Gurdon and Bourillot, 2001;Lawrence, 2001a). This review will focus on morphogen gradients in vertebrates with an emphasis on how studies using Xenopus have provided insight into both the existence of morphogens and their detailed mechanisms of action.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphogen gradients, positional information, and Xenopus: Interplay of theory and experiment

Green

2002

Developmental Dynamics

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The idea of morphogen gradients has long been an important one in developmental biology. Studies with amphibians and with Xenopus in particular have made significant contributions to demonstrating the existence, identity, and mechanisms of action of morphogens. Mesoderm induction and patterning by activin, nodals, bone morphogenetic proteins, and fibroblast growth factors have been analyzed thoroughly and reveal recurrent and combinatorial roles for these protein growth factor morphogens and their antagonists. The dynamics of nodal-type signaling and the intersection of VegT and ␤-catenin intracellular gradients reveal detailed steps in early long-range patterning. Interpretation of gradients requires sophisticated mechanisms for sharpening thresholds, and the activin-Xbra-Gsc system provides an example of this. The understanding of growth factor signal transduction has elucidated growth factor morphogen action and provided tools for dissecting their direct longrange action and distribution. The physical mechanisms of morphogen gradient establishment are the focus of new interest at both the experimental and theoretical level. General themes and emerging trends in morphogen gradient studies are discussed.

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Regulation and execution of apoptosis duringDrosophila development

Bangs

White

2000

Dev. Dyn.

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Morphogens, Compartments, and Pattern: Lessons from Drosophila?

Cited by 537 publications

References 96 publications

Genetic dissection of a stem cell niche: The case of the Drosophila ovary

Genetic dissection of a stem cell niche: The case of the Drosophila ovary

Morphogen gradients, positional information, and Xenopus: Interplay of theory and experiment

Regulation and execution of apoptosis duringDrosophila development

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