Morphohistological study of the different constituents of a banana ( Musa AAA, cv. Grande naine) embryogenic cell suspension

Georget, Frédéric; Domergue, Régis; Ferrière, Nicole; Côte, François-Xavier

doi:10.1007/s002999900188

Cited by 39 publications

(4 citation statements)

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“…These calli had a creamy yellow coloration and a granular and brittle structure (Fig. 4A) and are considered suitable for the establishment of cellular suspensions because they have been shown to initiate somatic embryos at a high frequency in coffee (Quiroz-Figueroa et al 2002;Teixeira et al 2004) and banana (Domergue et al 2000;Strosse et al 2003). NEC, on the other hand, are of crystalline and aqueous structure (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Cells with embryogenic potential are characterized as being small, isodiametric, having a big nucleus, evident nucleoli, dense cytoplasm, microvacuoles, and being rich in amyloplasts (Domergue et al 2000;Quiroz-Figueroa et al 2006;Namasivayam 2007), thus resembling the meristematic cells (Guerra et al 1999). In fact, embryogenic cell groupings contained in banana cell suspensions, the cells that form the embryo (embryonic), have few amyloplasts.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, embryogenic cell groupings contained in banana cell suspensions, the cells that form the embryo (embryonic), have few amyloplasts. Rather they are surrounded by typical embryogenic cells (Domergue et al 2000), establishing a source-sink relationship among the cells inside the meristematic region. This data was used to classify and evaluate the quality of embryonic cell suspensions (ECSs) in banana, but it did not allow detection of the molecular events that induce the morphological differentiation associated with embryogenic potential, which could be an even more fundamental factor of efficiency.…”

Section: Introductionmentioning

confidence: 99%

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A putative BABY BOOM-like gene (CaBBM) is expressed in embryogenic calli and embryogenic cell suspension culture of Coffea arabica L

Silva

Barduche

Livramento

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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The acquisition of embryogenic cell suspension (ECS) cultures has been one of the main objectives to maximize clonal propagation of the coffee plant. However, the majority of somatic embryogenesis induction requirements are genotype-dependent. Therefore, molecular markers linked to the embryogenic transition events may be useful. The BABY BOOM (BBM) gene can be considered as one of those markers, as it is related to the embryogenic process and to cell proliferation. BBM homologous sequences were obtained from Expressed Sequence Tags (ESTs) in a databank generated by the Brazilian Coffee Genome Project. We selected ESTcontigs that showed similarities with BBM sequence from different species. Two EST-contigs (C2 and C9) were expressed in silico in cellular suspension libraries and embryogenic calli of coffee. Contig C9, defined as BBM-like (CaBBM), presented similarity with BBM genes and showed 2-fold change in expression in ECS relative to embryogenic calli (EC). Contig C2, on the other hand, was related to the ERF-like family. It showed basal expression in nonembryogenic calli (NEC) and approximately 66-and 311-fold less in ECS and EC compared with CaBBM in the same samples, respectively. These data suggest that CaBBM is likely to be a BBM ortholog in Coffea arabica, which has potential for use as a molecular marker to further increase the methodological efficiency of in vitro culture of coffee.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A putative BABY BOOM-like gene (CaBBM) is expressed in embryogenic calli and embryogenic cell suspension culture of Coffea arabica L

Silva

Barduche

Livramento

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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“…The protocol described in our present study does not require any intermediate cell suspension system, making it a more convenient and very effective method for small laboratories lacking facilities for continuous agitation. According to Georget et al (2000), a common feature of many ba nan a c ell cu lt ur e p r ot oco ls is th e sl ow dev elo pme nt o f c ell cl ust ers, makin g pl ant regeneration time-consuming (18-24 months). The current protocol requires just 6 months for producing plantlets from the explant.…”

Section: Discussionmentioning

confidence: 99%

A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran

Lekshmi,

Soni,

Alex

et al. 2016

J. Hortic. Sci.

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A simple and rapid protocol for somatic embryogenesis in banana cv. Nendran (AAB) using immature male flowers (IMF) has been developed. The IMF produced palewhite to yellow, globular embryogenic callus on MS medium supplemented with BA (0.05 - 0.50mgL-1) and picloram (0.50 - 2.00mgL-1) with explant response of to 30 per cent. Addition of ascorbic acid (20mgL-1) and Gelrite© (0.45 per cent) to callus induction medium reduced interference from phenolic exudation. Embryogenesis was induced (33.3 to 60 per cent) on semisolid (0.30 per cent Gelrite©) MS medium supplemented with BA 2mgL-1 + IAA 0.5mgL-1. The somatic embryos showed 60-80 per cent germination on half- strength semisolid MS medium with BA 2mgL-1 + IAA 0.5mgL-1. Transfer of germinated embryos to semisolid MS medium supplemented with BA 2mgL-1 + NAA 1mgL-1under 14 h light /8h dark photoperiod resulted in hundred percent conversion to plantlets. This protocol takes merely 6 months for producing plantlets from immature flower buds through somatic embryogenesis, without any intermediate liquid cultures.

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Somatic Embryogenesis in Banana (Musa spp.)

López

Rayas

Medero

et al. 2022

Methods in Molecular Biology

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Morphohistological study of the different constituents of a banana ( Musa AAA, cv. Grande naine) embryogenic cell suspension

Cited by 39 publications

References 0 publications

A putative BABY BOOM-like gene (CaBBM) is expressed in embryogenic calli and embryogenic cell suspension culture of Coffea arabica L

A putative BABY BOOM-like gene (CaBBM) is expressed in embryogenic calli and embryogenic cell suspension culture of Coffea arabica L

A Rapid Protocol for Somatic Embryogenesis Mediated Regeneration in Banana (Musa Spp.) Cv. Nendran

Somatic Embryogenesis in Banana (Musa spp.)

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