Morphological analysis of collagen and elastic fibers in oral squamous cell carcinoma using special stains and comparison with Broder's and Bryne's grading systems

John, Rinju Elsa; Murthy, S T Srinivas

doi:10.4103/0970-9290.186242

Cited by 8 publications

(15 citation statements)

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“…The weak birefringence and greenish-yellow hue of the PDSCC samples could be either be due to an increased number of thin fibers (i.e., type III collagen fibers that were identified as reticulin fibers) or the result of abnormal or pathological collagen formed by the tumor cells or stroma. Regardless of the cause, our results were in accordance with those of other studies [ 3 , 6 , 7 , 16 , 17 , 31 ].…”

Section: Discussionsupporting

confidence: 93%

“…Previous studies have shown thata collagen-rich microenvironment can promote invasion and metastasis [ 30 ]. Similar to previous findings, the thickness of the collagen fibers decreased with progressing OSCC [ 6 , 31 ]. As cancer progresses, changes in the thickness of collagen fibers occurs with a decrease in the existing type I collagen and a simultaneous increase in type III collagen due to enzymatic degradation brought about by myofibroblasts [ 7 , 32 ].…”

Section: Discussionsupporting

confidence: 91%

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An Immunohistochemical and Polarizing Microscopic Study of the Tumor Microenvironment in Varying Grades of Oral Squamous Cell Carcinoma

Khalid

Siddiqui

Bordoloi

et al. 2018

J Pathol Transl Med

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BackgroundInvasion of epithelial cells into the connective tissue brings about massive morphological and architectural changes in the underlying stroma. Myofibroblasts reorganize the stroma to facilitate the movement of tumor cells leading to metastasis. The aim of this study was to determine the number and pattern of distribution of myofibroblasts and the qualitative and quantitative change that they cause in the collagen present in the stroma in various grades of oral squamous cell carcinoma (OSCC).MethodsThe study was divided into two groups with group I (test group, 65 cases) consisting of 29 cases of well-differentiated squamous cell carcinoma, 25 moderately differentiated SCC, and 11 poorly differentiated SCC, and group II (control group) consisting of 11 cases of normal mucosa. Sections from each sample were stained with anti–α-smooth muscle actin (α-SMA) antibodies, hematoxylin and eosin, and Picrosirius red. Several additional sections from each grade of OSCC were stained with Masson’s trichrome to observe the changes in collagen. For the statistical analysis, Fisher’s exact test, Tukey’s post hoc honest significant difference test, ANOVA, and the chi-square test were used, and p < .05 was considered statistically significant.ResultsAs the tumor stage progressed, an increase in the intensity α-SMA expression was seen, and the network pattern dominated in more dedifferentiated carcinomas. The collagen fibers became thin, loosely packed, and haphazardly aligned with progressing cancer. Additionally, the mean area fraction decreased, and the fibers attained a greenish yellow hue and a weak birefringence when observed using polarizing light microscopy.ConclusionsMyofibroblasts bring about numerous changes in collagen. As cancer progresses, there isincrease in pathological collagen,which enhances the movement of cells within the stroma.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

An Immunohistochemical and Polarizing Microscopic Study of the Tumor Microenvironment in Varying Grades of Oral Squamous Cell Carcinoma

Khalid

Siddiqui

Bordoloi

et al. 2018

J Pathol Transl Med

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“…During malignant transformation, elongated projection of the core of the papilla from the LP into the tumor has been noted in histology, which presents highly retardant spikes seen in cross-sectional, cumulative PS-OCT ( Figure 3 B,G). These retardant changes in PS-OCT are consistent with previous studies using Picrosirius red staining and polarized light microscopy, which have shown densely packed collagen fibers with high birefringence surrounded the tumor islands in well-differentiated tumors [ 18 , 19 , 27 , 28 ].…”

Section: Discussionsupporting

confidence: 91%

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Chen

Lee²,

Chen³

et al. 2020

Cancers

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Detection of oral dysplastic and early-stage cancerous lesions is difficult with the current tools. Half of oral cancers are diagnosed in a late stage. Detection of early stromal change to predict malignant transformation is a new direction in the diagnosis of early-stage oral cancer. The application of new optical tools to image stroma in vivo is under investigation, and polarization-sensitive optical coherence tomography (PS-OCT) is potentially one of those tools. This is a preliminary study to sequentially image oral stromal changes from normal, hyperplasia, and dysplasia to early-stage cancer by PS-OCT in vivo. We used 4-Nitroquinoline-1-oxide drinking water to induce dysplasia and early-stage oral cancer in 19 K14-EGFP-miR-211-GFP transgenic mice. A total of 8 normal, 12 hyperplastic, 11 dysplastic, and 4 early-stage cancerous lesions were enrolled. A new analytic process of PS-OCT imaging was proposed, called an en-face birefringence map. From the birefringence map, the sensitivity, specificity, positive predictive value, and negative predictive values to detect dysplasia and early-stage cancer were 100.00%, 95.00%, 93.75%, and 100.00%, respectively, and the kappa value of these images between two investigators was 0.942. The mean size of malignant lesions detected in this study is 1.66 ± 0.93 mm. This pilot animal study validates the use of PS-OCT to detect small and early-stage oral malignancy with high accuracy and consistency.

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“…However, we cannot disregard that MFs are also capable of producing MMPs, inducing epithelial-to-mesenchymal transition, E-cadherin dysregulation, and increasing cellular invasiveness. 18,27 In ACs, interestingly, the results of this study show that the more SE penetrates the connective tissue and approaches the dysplastic lin- The lack of detailed clinical information was a limitation of this study since we often found incomplete medical records. Also, because the LLSCCs and ACs were histopathologically graded, a higher number of cases could have brought more benefits to work, although there is agreement with the sample calculation performed for this study.…”

Section: Discussionmentioning

confidence: 88%

“…In addition, larger tumors (T3/4) also have lower density of elastic fibers on the tumor front. Similarly, studies have shown that Oral Squamous Cell Carcinoma (OSCC) with lower amounts of collagen and elastic fibers in the invasive borders have more aggressive characteristics, 17,18 and their absence was observed in cases that presented lymph node metastasis 19 …”

Section: Discussionmentioning

confidence: 98%

Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma

Martinez,

Bezerra,

Alves

et al. 2024

J Oral Pathology Medicine

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BackgroundTo evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs).MethodsSamples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF‐β, α‐SMA, and Ki‐67 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann–Whitney, Kruskal‐Wallis/Dunn, and Spearman correlation tests (SPSS, p < 0.05).ResultsACs showed higher number of α‐SMA+ MFs when compared to CG (p = 0.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (p = 0.027). Areas of SE had lower deposits of collagen (p < 0.001), immunostaining for TGF‐β (p < 0.001), and higher density of elastic fibers (p < 0.05) when compared to areas without SE. A positive correlation was observed between high‐risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (p = 0.027). LLSCCs showed a higher number of α‐SMA+ MFs about CG (p = 0.034), as well as a reduction in the deposition of total collagen (p = 0.009) in relation to ACs and CG. There was also a negative correlation between the amount of α‐SMA+ cells and the accumulation of total collagen (p = 0.041). Collagen and elastic density loss was higher in larger tumors (p = 0.045) with nodal invasion (p = 0.047).ConclusionsOur findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.

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Morphological analysis of collagen and elastic fibers in oral squamous cell carcinoma using special stains and comparison with Broder's and Bryne's grading systems

Abstract: The study of the connective tissue stromal changes can be used as an adjunct to histological grading and thereby helping the surgeon to determine the amount of marginal clearance.

Cited by 8 publications

References 0 publications

An Immunohistochemical and Polarizing Microscopic Study of the Tumor Microenvironment in Varying Grades of Oral Squamous Cell Carcinoma

An Immunohistochemical and Polarizing Microscopic Study of the Tumor Microenvironment in Varying Grades of Oral Squamous Cell Carcinoma

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma

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