Morphological and Biochemical Changes in Myelin Subfractions of Developing Rats Fed Microbial Lipids

Salvati, Serafina; Vergiliis, L Conti de; Felice, Maurizio Di; Gier, J. De; Demel, R.A.; Crescenzi, G. Serlupi; Tomassi, G.; Tagliamonte, B

doi:10.1111/j.1471-4159.1984.tb02729.x

Cited by 17 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Developing rats deprived of dietary essential fatty acids have reduced brain myelin content and myelin protein levels, and the fatty acid patterns of myelin lipids are altered [Galli et al, 1972;Gozzo and D'Udine, 1997;McKenna and Campagnoni, 1979;Trapp and Bernsohn, 1978]. The type of lipid fed is also associated with morphological and biochemical changes in myelin subfractions of developing rats [Salvati et al, 1984;Srinivasaro et al, 1997], and fat intake influences the developmental increase in the mRNA levels for proteolipid protein, myelin basic protein and stearoyl CoA desaturase in the brains of neonatal mice [DeWille and Farmer, 1992]. Confaloni et al [1993] suggest that dietary lipids or their metabolites play a direct role in transducing intra-or extracellular signals, triggering cellular events that regulate the differentiation, proliferation and migration of oligodendrocytes, from which myelin is derived.…”

mentioning

confidence: 99%

Effect of Docosahexaenoic Acid Content of Maternal Diet on Auditory Brainstem Conduction Times in Rat Pups

et al. 2000

View full text Add to dashboard Cite

Previous studies of dietary docosahexaenoic acid (DHA; 22:6n–3) effects on neurodevelopment have focused mainly on effects on the visual system; these studies may be confounded by effects on the retina rather than on neural pathways. Auditory brainstem conduction times (ABCTs) provide an alternate measure of central neural development. We conducted a dose-response study in which ABCTs were measured in pups whose dams were fed diets containing one of three levels of DHA (2, 4 or 6% of total fatty acids) from a single cell oil. Diets were fed during pregnancy and lactation, and pups were randomly cross-fostered on postnatal day 3 to minimize litter effects. ABCTs showed a dose-response effect, with higher levels of dietary DHA being associated with longer conduction times on postnatal day 31 (p < 0.05). Higher dietary DHA was reflected in pup cerebrums collected on postnatal days 3 and 31, and levels of arachidonic acid (AA, 20:4n–6) were inversely related to levels of DHA. This study demonstrated that the auditory brainstem response is sensitive for identifying effects of diet on neurodevelopment, and that supplementing the maternal diet with high levels of DHA may negatively impact development of the central auditory system of offspring.

show abstract

mentioning

confidence: 99%

Effect of Docosahexaenoic Acid Content of Maternal Diet on Auditory Brainstem Conduction Times in Rat Pups

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Our data show that MBP-and PLP-positive fibers in the telencephalon area are already present at 7 days postnatally in the test rats, indicating that the lipid fraction extracted from yeast grown on n-alkanes interferes with brain development, accelerating myelinogenesis, and that these changes can occur in the absence of any signifi cant changes in food consumption or growth rate [12][13][14][15][16] (data not shown).…”

Section: R E S U Ltsmentioning

confidence: 87%

Accelerated Myelinogenesis Induced by Dietary Lipids in Rats

Confaloni

Avellino²,

Campeggi³

et al. 1993

Dev Neurosci

Self Cite

View full text Add to dashboard Cite

Our previous work has shown an early development of behavioral reflexes in the offspring of rats maintained on diets containing a lipid fraction extracted from yeast (Candida lypolitica) grown on n-alkanes during pregnancy and throughout lactation. Since some of these changes could be linked to an early myelination, in this study we investigated myelin maturation in the rat brain by immunohistochemistry. At 7 days the test groups showed considerable immunopositivity to myelin basic protein and proteolipid protein at a more rostral level, such as the corpus callosum where immunopositivity is usually detected later in brain development. At 7 days in controls, staining fibers were detected only in the lower brainstem and in the cerebellum. Immunopositivity in the same regions was more intense in the test groups. Some litters were fostered at birth to produce 2 groups of animals: pups whose mothers were fed a control diet prenatally and the test diet postnatally, and vice versa. Positive fibers are already present at 7 days in the telencephalon area in both groups. These data indicate that dietary lipids can interfere with brain development by accelerating myelinogenesis.

show abstract

“…In this fraction, changes in the basic proteins percentage were observed (Salvati, 1984). The same fraction was affected in Lewis rats in which acute EAE was induced, where a decrease of basic proteins and morphological changes was observed (Salvati et al, 1986).…”

Section: Changes In Light Myelin Fraction In Cr-eaementioning

confidence: 87%

Biochemical changes in central nervous system membranes in chronic-relapsing experimental allergic encephalomyelitis

Salvati

Confaloni

DiBiase

et al. 1990

Molecular and Chemical Neuropathology

View full text Add to dashboard Cite

Biochemical studies of myelin fractions were undertaken on Lewis rats during various time-points in the development of chronic-relapsing experimental allergic encephalomyelitis (CR-EAE). Lipid and protein composition of myelin fractions obtained by sucrose density gradient centrifugation at 10, 19, 24, and 66 d postinduction (pi) were determined by high-performance thin-layer chromatography (HPTLC) and sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS PAGE), respectively. When comparing the myelin fractions of CR-EAE affected animals with those of controls, main differences were observed at 10 d pi. These changes were particularly evident in the light myelin fraction, where a decrease in the percentage of phosphatidylethanolamine and small basic protein relative to the total lipids and proteins of the fraction were observed. At 19 and 24 d pi no biochemical differences were present in both fractions. At 66 d pi, differences in the lipid composition were observed again only in the light myelin fraction. These findings suggest that the light myelin fraction is the most sensitive, particularly at the early stages of the disease, and must play a key role in demyelinating processes.

show abstract

Morphological and Biochemical Changes in Myelin Subfractions of Developing Rats Fed Microbial Lipids

Cited by 17 publications

References 34 publications

Effect of Docosahexaenoic Acid Content of Maternal Diet on Auditory Brainstem Conduction Times in Rat Pups

Effect of Docosahexaenoic Acid Content of Maternal Diet on Auditory Brainstem Conduction Times in Rat Pups

Accelerated Myelinogenesis Induced by Dietary Lipids in Rats

Biochemical changes in central nervous system membranes in chronic-relapsing experimental allergic encephalomyelitis

Contact Info

Product

Resources

About