Morphological and Biochemical Changes Which Occur During Postnatal Development and Maturation of the Rat Testis1

Mills, Nathaniel; Mills, Teresa M.; Means, Anthony R.

doi:10.1095/biolreprod17.1.124

Cited by 21 publications

(8 citation statements)

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“…2) and the body and testicular weight and diameters of seminiferous tubules and Leydig cells nuclei were significantly elevated (Table I, Fig. 6) as previously reported (3,24).…”

Section: Resultssupporting

confidence: 86%

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

2002

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Compound CDRI 84/35 (a piperazine derivative--a potent antispermatogenic agent) has been shown to cause significant inhibition in testicular spermatogenesis without affecting Leydig cell and accessory sex organ function in adult rats. The present study was conducted to determine its effect on the germ cell population and Leydig cell morphology in immature rats (40-50 gm) administered CDRI 84/35 (100 mg/kg/day p.o.), synthetic estradiol benzoate (EB; 5 microg/rat/day) and vehicle at the age of 21 days. Animals were killed 24 h later following 7 and 14 days' treatments. Bouin's fixed testes were sectioned (at 5 microm) and stained with PAS-hematoxylin. Quantitative determination of Sertoli Cell-Germ Cell ratio was carried out in 150 round seminiferous tubules in each group of 5 rats. Results revealed a significant decrease in number of the spermatocytes (non-pachytene and pachytene) and early (round) spermatids in step 1-8 of spermiogenesis without affecting Leydig cell morphology in rats administered CDRI 84/35 for 7 and 14 days as compared to corresponding controls. In contrast, the testes of rats injected with synthetic EB, caused a marked inhibition in these meiotic and post-meiotic germ cell types, as well as in the diameters of round seminiferous tubules, and Leydig cells nuclei (only in 14 days treatment), and testicular weight on autopsy days 8 and 15 as compared to CDRI 84/35-treated rats. While the number of pre-meiotic spermatogoniae was observed to be slightly decreased after only 14 days treatment in both CDRI 84/35 and EB treatment groups, the Sertoli cell number did not show any significant change as compared to controls. The present investigation confirms the antispermatogenic effect of compound CDRI 84/35 in immature rats similar to that reported in adult rats. Marked inhibition in pachytene spermatocytes and other testicular parameters following synthetic estrogen treatment might be due to its antiandrogenic action, contrasting with the non-hormonal profile of CDRI compound.

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“…2) and the body and testicular weight and diameters of seminiferous tubules and Leydig cells nuclei were significantly elevated (Table I, Fig. 6) as previously reported (3,24).…”

Section: Resultssupporting

confidence: 86%

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

2002

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“…At this time, important events occur in the developing testes, such as Leydig cell differentiation, rapid tubular growth and com¬ pletion of the first spermatogenic wave (Mills et al, 1977;Chemes et al, 1979). The nuclear matur¬ ational changes of Sertoli cells are coincident with these high hormonal levels.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of Sertoli cells in neonatally oestrogen-treated rats

Gaytan¹,

Aguilar²

1987

Reproduction

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Summary. On Day 1 of age rats were treated with 500 \g=m\g oestradiol benzoate.Oestrogen-treated rats had increased numbers of Sertoli cells per reference area or volume, whereas the total number of cells per testis was unchanged. The mean nuclear size was significantly smaller in oestrogen-treated rats than in control rats, at 22 and 45 days of age. The volume density of the heterochromatin clumps decreased from 22 to 45 days of age in control rats (68% fall), the decrease being slower in oestrogenized animals (30% fall) during the same period. The differences were significant at 45 days of age only. The relative volume occupied by the nuclear membrane infoldings was significantly less in oestrogenized rats than in control ones at the two ages considered. Nucleolar development was delayed in oestrogen-treated rats, which had lower numbers of nuclear sections showing nucleoli, as well as a decrease in the nucleolar diameter. We suggest that these Sertoli cell alterations are due to the altered gonadotrophin and testosterone concentrations induced by the steroid treatment rather than to a direct effect of oestrogen.

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“…As early as postnatal day 10 some tubular lumina are open in normal rats (Russell et al, 1989) and continue to grow as the SEH and the TD increase (Hodgen and Sherins, 1973;Mills et al, 1977). Therefore, significant rises in TD/SEH values from day 22 through 60 would be unexpected in control rats, but these values might be altered in rats treated neonatally with estrogens.…”

Section: Morphometrymentioning

confidence: 99%

Neonatal exposure of male rats to estradiol benzoate causes rete testis dilation and backflow impairment of spermatogenesis

et al. 1998

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Estrogens administered to perinatal rodents cause spermatogenesis impairment; this study was undertaken to determine the mechanisms by which estrogens exert this effect. Neonatal male Wistar rats received estradiol benzoate (either 0.5 mg/5g BW or 1 mg/5g BW) and were killed at days 10, 22, 33, 45, and 60. Controls received vehicle. In tubule cross-sections of transverse sections of the right testes, 1) tubular diameter (TD) and seminiferous epithelium height (SEH) were measured, 2) normal and impaired spermatogenesis were classified in terms of the most advanced germ cell type present, including tubules lined by Sertoli cells only. A significant dose-dependent rise in the tubule percentage lined by Sertoli cells only at day 60 reflected spermatogenesis impairment. This was evidenced by the presence of multinucleated germ cells in a thin epithelium and sloughed into an enlarged tubular lumen, which was reflected in a significant dose-dependent increase in TD/SEH values from day 22 onward. TD was significantly greater and SEH significantly lower in tubular segments located at the cranial than the caudal halves of rat testes treated with the high (days 22, 33, and 60) and the low dose (day 33). This indicated distension in cranial tubular segments, perhaps due to the fact that these segments were the closest to the dilated rete testis. Consequently, they showed the highest TD/SEH values and the most regressive features of spermatogenesis (tubules lined by Sertoli cells only). In contrast, caudal segments in rat testes treated with the low dose showing TD/SEH values similar to controls displayed a delayed maturation of spermatogenesis coinciding with the late appearance of mature Leydig cells.

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Morphological and Biochemical Changes Which Occur During Postnatal Development and Maturation of the Rat Testis1

Cited by 21 publications

References 0 publications

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

Effect of Compound Cdri 84/35 and Synthetic Estrogen on the Seminiferous Epithelium of Immature Rat

Quantitative analysis of Sertoli cells in neonatally oestrogen-treated rats

Neonatal exposure of male rats to estradiol benzoate causes rete testis dilation and backflow impairment of spermatogenesis

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