Morphological and Histochemical Observations on the Primordial and Early Growing Oocytes of Crow (Corvus splendens) and Myna (Acridotheres tristis)

Abstract

A morphological and histochemical study has been made of the primordial and early growing oocytes in the ovaries of crow (Corvus splendens) and common myna (Acridotheres tristis). The primordial oocytes in the myna ovary are loosely arranged in groups or nests, whereas in crow they form compact nests surrounded by highly vascularized connective tissue bands or lie in layers beneath the surface epithelium. The primordial oocytes in both the species are surrounded by flat granulosa cells whose number, shape, and cytochemical properties change with the initiation of growth. The oocyte nucleus shows a single basophilic nucleolus and thick diplotene chromosomes. With the initiation of growth, the number of nucleoli increases; simultaneously the chromosomes attain lampbrush configuration. Crescent-shaped Balbiani's vitelline body consists of ribonucleoproteins, lipoproteins, and phospholipids. The amount of these substances increases with the oocyte growth. The nature of proteins and lipids in the ooplasm and follicular epithelium also changes with the oocyte growth. Some randomly distributed protein bodies are also present in the ooplasm of primordial follicles. They disappear with the initiation of oocyte growth. The enzyme activities of acid phosphatase, NADP-diaphorase and NAD-diaphorase, also increase in the Balbiani's vitelline body with the oocyte growth. Alkaline phosphatase and delta 5-3 beta-HSDH activities are not seen. The possible functional significance of these morphological and histochemical changes has been discussed in relation to the initiation of growth in quiescent oocytes.

Ultrastructural Localization of Acid Phosphatase in Germ Cells of Chick Embryo Left Ovary

“…This author located it in lysosomes and in some of the RER cis ternae. Chalana and Guraya [1979] revealed its presence histochemically in the Balbiani body of two avian species. Very recently.…”

Developmental Stages of Primary Oocytes in Turkeys

“…Although endocrine and morphological events associated with the rapid growth phase of the hen's ovarian follicle have been documented (Marza and Marza, 1935;Perry et at., 1978a,b;Chalana and Guraya, 1979;Yoshimura et al, 1993), considerably less is known of the initial stages in the development of the early primary oocyte in the slow growth stage. Furthermore, nothing is known about the early primary oocyte in the slow growth stage of the The Balbiani body was partially dispersed by Stage III (151 to 350 itm) and associated organelles appeared in clusters in the ooplasm.…”

Structural and cuantitative aspects of the ovary of the Fulica armillata (Aves: Rallidae)

“…Están formados por el ovocito y la pared folicular, estructuras ovocitarias que exhiben las notorias modificaciones producidas durante la maduración de los mismos. Los procesos de foliculogénesis y vitelogénesis han sido estudiados en el ovario de diferentes especies silvestres (Chalana & Guraya 1979, Guraya 1989a, Madekuroswa & Kimaro 2006). Speroni (2003) y Bulfon (2008) analizaron las variaciones estructurales y cuantitativas durante el período reproductivo anual de aves estacionales como Spheniscus magellanicus, Myiopsitta monachus, e Himantopus melanurus.…”