Morphological and molecular characteristics of foliar nematode attacking silver birch (Betula pendula Roth) in Poland

Abstract

SummaryAphelenchoides fragariae (Ritzema Bos, 1890) Christie, 1932 was isolated from leaves of silver birch (Betula pendula Roth) seedlings proving that the source of infection was anemones plants. This is the fi rst report to our best knowledge showing that the source of nematode infection of a woody plant could be a perennial plant. A. fragariae was identifi ed by morphometric and molecular analyses. Morphological diagnosis based on the bending shape of the tail of males and pronounced apex and rostrum proved to be the most accurate reliable characteristic. On the opposite, the high variability of the mucron shape in female tails made the identifi cation by microscopic analyses diffi cult. Identifi cation of the species was confi rmed by analysis of 28S rDNA sequences. The morphometric data of adults extracted from silver birch was compared with that of nematodes isolated from Anemone hupehensis (Lemoine) Lemoine. Males body length varied highly in samples collected from both host plant species.

Populations of parasitic nematodes colonizing Jerusalem artichoke (Helianthus tuberosus L.)

“…1). The initial symptoms of infection in Jerusalem artichoke leaves were irregular, water-containing lesions between leaf veins, which spread gradually and caused necrotic changes in plant tissues -symptoms that are very similar in both infested Jerusalem artichoke [15] and in other hosts, for example silver birch [28], among diverse ornamental plants [29][30][31]. The genus Aphelenchoides Fischer, 1894 is composed of ubiquitous fungi-feeding species, as well as species that infect insects and plants.…”

“…These nematodes feed on both fungi and plants [32]. Numerous plant species are colonized by the foliar nematodes [28] that are the most widespread parasitic nematodes in Poland. Nematodes of the genus Aphelenchoides are usually transmitted via clothing, footwear, containers, packaging (wood), land vehicles, paper, soil, sand, and gravel [33].…”

Rapid Detection of the Strawberry Foliar Nematode Aphelenchoides fragariae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks

“…Sequences of the nuclear ribosomal genes 18S rRNA, ITS rRNA and D2-D3 of 28S rRNA are clearly differentiate A. fragariae from other nematodes [20][21][22][23][24][25][26][27]. Ibrahim et al [28] were the first to provide PCR-ITS-RFLP profiles for A. fragariae and other Aphelenchoides spp.…”