Morphological and molecular characterization of <i>Strongyloides ophidiae</i> (Nematoda, Strongyloididae)

Santos, Karina Rodrigues dos; Carlos, Bianca Cechetto; Paduan, K. S.; Kadri, Samir Moura; Barrella, T. H.; Amarante, Mônica Regina Vendrame; Ribolla, Paulo Eduardo Martins; Silva, Reinaldo José da

doi:10.1017/s0022149x09990381

Cited by 13 publications

(16 citation statements)

References 12 publications

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“…The genus Strongyloides contains more than 40 species of parasitic monoxenous nematodes (Grove, 1989) that mainly infect mammals, and more occasionally reptiles, amphibians and birds (Dorris et al , 2002; Viney & Lok, 2007). Among reptiles, although snakes are the group most commonly infected (Pereira, 1929; Holt et al , 1979; Dos Santos et al , 2010), geckos ( Hemydactylus mabouia , Rodrigues, 1968) and lacertids ( Podarcis pityusensis , Roca & Hornero, 1992) have also been reported as hosts. The screening of tissue samples in this study has revealed the presence of Strongyloides in a new host, the lacertid lizard P. lilfordi .…”

Section: Discussionmentioning

confidence: 99%

“…The lack of sequences from other Strongyloides species infecting reptiles prevents confirmation of host-specificity within this group. However, an assessment including a short fragment (323 bp) in GenBank available for a species infecting a snake ( S. ophididae , accession number EU287935, Dos Santos et al , 2010 – analysis not shown) indicates that the two sequences available from Strongyloides species infecting reptiles and the one from Strongyloides sp. infecting frogs are part of the same clade.…”

Section: Discussionmentioning

confidence: 99%

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Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences

Perera¹,

Maia²,

Jorge³

et al. 2012

J. Helminthol.

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The development of molecular methods is becoming a promising field in the analysis of parasite fauna in wildlife species. This is especially useful in the case of parasite species where developmental larval stages are difficult to assess using standard methods. In this study we screened for the presence of parasitic nematodes infecting lacertid lizards from the Iberian Peninsula and Balearic islands using nematode-specific 18S ribosomal RNA gene primers on host tissue samples. Sequencing of positive samples revealed the presence of different genera of nematodes. The detection of Strongyloides, a monoxenous genus reported for the first time in Podarcis lilfordi is probably the result of the amplification of larval stages present in the host circulatory system. Two spirurid nematodes, Synhimantus and a new unidentified clade, were also found, suggesting that reptiles might be paratenic hosts of several spirurid species. This study demonstrates the benefits of using specific molecular markers on tissue samples to identify infecting larval stages of nematodes, otherwise difficult to assess using traditional screening methods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences

Perera¹,

Maia²,

Jorge³

et al. 2012

J. Helminthol.

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“…Podem ocasionar úlceras hemorrágicas, inflamação severa, anemia, peritonite, infecções secundárias e fezes muco-sanguinolentas (Klingenberg, 1993;Dias et al, 2004). Pinto et al (2010) observaram o parasito Kalicephalus costatus no intestino de uma serpente da espécie Crotalus durissus, a qual pertencia a um sistema semi-intensivo de cativeiro no Brasil, assim como Silva et al (2001). A espécie K. inermis foi reportada no estômago de outro exemplar desta serpente, neste caso, de vida livre.…”

Section: Helmintosunclassified

“…Esta sintomatologia é somente observada em animais com infecção bacteriana secundária, decorrente de um alto grau de parasitismo, estresse, higiene inadequada e umidade relativa e temperatura elevadas (Klingenberg, 1993;Luppi et al, 2007). Luppi et al (2007) Siqueira et al (2009), e em Crotalus durissus de sistema semi extensivo (Silva et al, 2001).…”

Section: Helmintosunclassified

Parasitismo em serpentes

et al. 2020

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A criação cativa de serpentes tem sido cada vez mais intensificada devido ao seu uso como pet, conservação de animais selvagens, importância em pesquisas, desenvolvimento tecnológico, produção de soro antipeçonha e outros produtos. O parasitismo nestes animais é frequente, podendo atingir alta carga parasitária, a qual pode ser intensificada com o estresse de cativeiro que leva à depressão do sistema imunológico. Os parasitos não apenas influenciam na viabilidade dos animais mantidos em cativeiro, como também podem ter potencial zoonótico. Esta revisão visa auxiliar profissionais da área na manutenção adequada de serpentes em cativeiro.

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“…Under indirect development, the rhabditiform larvae develop into either males or females. Postembryonic development of Strongiloides has distinct morphological traits by which its stages are identified (Lyons et al, 1973;Dewes and Townsend, 1990;Grant et al, 2006;Viney, 2006;Santos et al, 2010;Thamsborg et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Morphological features of development of Strongyloides westeri (Nematoda, Rhabditida) in vitro

Gugosyan

Yevstafyeva

Горб

et al. 2018

Regul. Mech. Biosyst.

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Strongyloides westeri (Ihle, 1917), a parasitic horse nematode, has an unusual lifecycle, which allows it to exist for a long time in the environment. Morphometric features of eggs, larvae and free-living S. westeri were studied in vitro under different temperature regimes. The optimal temperature for their embryonic development is 25 °С, under which 90% of the first stage rhabditiform larvae are formed and released within 7 hours of cultivation. The temperatures of 20 and 30 °С are less favorable for their development. Embryonic development of Strongyloides has four stages that differ in morphology and size. The eggs of a parthenogenetic female are 3.7% longer and 19.6% wider than eggs isolated from free-living females of S. westeri. In embryogenesis, the eggs shorten by 4.4 μm (6.5%) and widen by 5.35 μm (8.3%). New data were obtained on postembryonic development of S. westeri. The differential morphometric features of stage 1 and 2 rhabditiform larvae which grow both in length and width (33.7% and 30.4% respectively) are established. The development of filariform larvae is associated with loss of bulbous thickening and formation of cylindrical oesophagus. Simultaneously, the body elongates, and the gut becomes shorter. Differential morphometric features of free-living males and females of S. westeri are the length and width of body, length of oesophagus, gut, tail end, and size of spicules. Postembryonic development of the free-living and parasitic generations from rhabditiform larvae is temperature-dependent. Most of the free-living generations of Strongyloides (54.0%) are formed at 20 °С, and filariform larvae mostly (70.0%) develop at 30 °С. The obtained results of morphological studies improve differential diagnostics of the nematode at various stages of development and further advance the study of its intraspecific variability.

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Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)

Cited by 13 publications

References 12 publications

Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences

Molecular screening of nematodes in lacertid lizards from the Iberian Peninsula and Balearic Islands using 18S rRNA sequences

Parasitismo em serpentes

Morphological features of development of Strongyloides westeri (Nematoda, Rhabditida) in vitro

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