Morphological and Molecular Characterization of Gastrointestinal Nematodes in Sheep

Veena, M; Puttalakshmamm, G. C.; Ananda, K. J.; Sengupta, P. P.

doi:10.20546/ijcmas.2020.904.308

Cited by 2 publications

(1 citation statement)

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“…Conventional PCR targeting the internal transcribed spacer 2 gene with genus-specific primer pairs ( Table-1 ) [ 27 , 28 ] was used to detect resistant nematodes, namely, Haemonchus spp., Oesophagostomum spp., Trichostrongylus spp., and Teladorsagia spp. The PCR assays were conducted in a 25 μL reaction volume, using 12.5 μL HotStar Taq PCR (Qiagen, Valencia, CA) master-mix, 8.5 μL double distilled water, 1 μL forward primer, 1 μL of reverse primer, and 2 μL DNA template.…”

Section: Methodsmentioning

confidence: 99%

Assessment of gastrointestinal nematode anthelmintic resistance and acaricidal efficacy of fluazuron-flumethrin on sheep and goat ticks in the North West province of South Africa

Emsley,

Matshotshi,

Mathebula

et al. 2023

Vet World

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Background and Aim: Anthelmintic resistance (AR) and acaricide resistance (ACR) pose great economic threat to communal livestock raised by rural communities, limiting sustainable production. This study was conducted to assess the occurrence of AR and ACR against nematodes and ticks that infest small ruminants (sheep and goats) from small-scale farming communities in the North West Province of South Africa, as well as document the associated risk factors. Materials and Methods: The study was conducted on small-scale farming locations in two districts of the North West Province, namely, Dr. Ruth Segomotsi Mompati district and Dr. Kenneth Kaunda district, from November 2019 to March 2020. A questionnaire survey based specifically on antiparasitic treatment and related management practices was administered to 86 small-scale farmers. A fecal egg count reduction test (FECRT) was used to determine in vivo AR in small ruminants against benzimidazole (BZD), levamisole, and macrocyclic lactone on nine ruminant farms. Then, deoxyribonucleic acid was extracted from L3 larvae and resistant nematodes were identified using a polymerase chain reaction, targeting the internal transcribed spacer 2 gene. An egg hatch assay (EHA) and a larval mortality assay (LMA) were used to determine in vitro AR against thiabendazole (TBZ and BZD) in the same farms. Acaricide resistance against fluazuron–flumethrin (Drastic Deadline eXtreme) pour-on was assessed using an adult immersion test (AIT) on Rhipicephalus evertsi. Results: Questionnaire results indicated that most farmers (89%) relied solely on anthelmintics. Farmers used visual appraisal to estimate the dosage, which is the primary cause of resistance. The FECRT revealed AR in all the farms. Egg hatch assay results revealed AR development against TBZ in all districts, with >95% of the eggs hatching at variable doses. Larval mortality assay results revealed the development of resistance against BZD, with 50% of L3 larvae surviving at different doses in all farms. Adult immersion test results indicated that fluazuron-flumethrin (>99%) exhibited high acaricidal efficacy against R. evertsi by inhibiting tick oviposition. Conclusion: This investigation found that sheep and goats in the studied areas are developing AR to gastrointestinal parasites. The findings of in vivo tests showed resistance with fecal egg count reduction percentage of <95% or lower confidence limit of <90%. The results of EHA and LMA revealed no evidence of inhibition of egg development and larval mortality, indicating the development of resistance. Acaricide resistance was not detected against fluazuron–flumethrin, which is commonly used in the study areas. Thus, developing management methods for these economically significant livestock nematodes, including teaching small-scale farmers how to properly administer anthelmintics and acaricides to their livestock, is urgently needed. Keywords: acaricidal resistance, adult immersion test, anthelmintic resistance, egg hatch assay, gastrointestinal nematodes, larval mortality assay.

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Section: Methodsmentioning

confidence: 99%