Morphological and molecular characterization of Pyricularia oryzae isolates causing wheat blast in Bangladesh

Noman, Md. Abdullah Al; Hosen, Sarowar; Shamsi, Shamim

doi:10.1007/s42360-020-00300-4

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…The morphology of all isolates was characterized based on upper and lower colonies, growth rate, conidial size and conidiophores. The colony appearance described in the present study was in accordance with studies by Al Noman et al (2021) and Hussin et al (2020). Moreover, the colony growth of all P. oryzae isolates grown on PDA plates was 4.87 ± 0.02 cm on day 7, which was in line with the findings by Al Noman et al (2021) and Castroagudín et al (2016) that stated P. oryzae can grow in a range of 4.3 cm and reach up to 8.4 cm on day 14.…”

Section: Discussionsupporting

confidence: 92%

“…The conidial shape of Pyriculariaceae varies greatly depending on the host (Longya et al, 2020). The conidial features in this study showed similarities with Al Noman et al (2021), Castroagudín et al (2016 and Klaubauf et al (2014). These studies stated pyriform shaped conidia reported as P. oryzae with the conidial size in the range of 17-28 µm × 6-8 µm.…”

Section: Discussionsupporting

confidence: 63%

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Morphological and molecular identification of Pyricularia oryzae causing blast disease on rice (Oryza sativa)

Shukri, I. A. M.,

Yahya, A. R. M.,

Mohd, M. H.

et al. 2023

MJM

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Aims: Rice (Oryza sativa) is one of Malaysia's most significant crops. Rice blast caused by Pyricularia oryzae is one of the most serious diseases of Oryza sativa, causing significant damage to the Malaysian rice crop and impacting productivity. This study was carried out to isolate and characterize phytopathogenic fungal isolates associated with rice blast collected in a paddy field in Alor Setar, Kedah, Malaysia. Methodology and results: Morphological characterization of seven fungal isolates obtained showed thin, white, and grayish green mycelia and the reverse colony was light yellow to brown. The fungal isolates produced two-septate pyriform (pear-shaped) conidia with solitary, unbranched and light brown conidiophores. Pathogenicity tests of all isolates on rice leaves revealed diamond-shaped symptoms with a grayish center and brown edge. All isolates showed similar morphological and pathogenicity characteristics; thus, a representative isolate was further identified through DNA sequencing and phylogenetic analysis of the internal transcribed spacer (ITS) region for species confirmation. Based on DNA sequences of ITS and phylogenetic analysis, the representative isolate was confirmed as P. oryzae. Conclusion, significance and impact of study: Seven isolates morphologically identified as Pyricularia sp. were tested as pathogenic by causing rice blast disease. Representative isolate P2 (USM-PD1) was confirmed to be P. oryzae by DNA sequencing and phylogenetic analysis of the ITS region. This study provides information on the etiology and symptomatology of rice blast disease caused by P. oryzae USM-PD1 that can be applied to diagnose and mitigate the threat posed by this plant pathogen for the disease management.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 63%

Morphological and molecular identification of Pyricularia oryzae causing blast disease on rice (Oryza sativa)

Shukri, I. A. M.,

Yahya, A. R. M.,

Mohd, M. H.

et al. 2023

MJM

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“…Infected rice tissues were collected and placed in a paper envelope with an identity label indicating the type of variety, area/location, and date of collection for future use. The blast pathogens were isolated using the method described in [16]. Macroscopic characterization of the blast pathogen isolates was performed using the texture and color of each isolate's colonies on PDA and OMA media.…”

Section: Isolation and Identification Of The Fungus P Oryzaementioning

confidence: 99%

“…Macroscopic characterization of the blast pathogen isolates was performed using the texture and color of each isolate's colonies on PDA and OMA media. P. oryzae is distinguished by the presence of club-shaped conidia with two septa, though one or three septa are occasionally found [16].…”

Section: Isolation and Identification Of The Fungus P Oryzaementioning

confidence: 99%

Population structures of Pyricularia oryzae Cavara in South Sulawesi

Kuswinanti

Patandjengi

Amin

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Blast disease caused by Pyricularia oryzae is one of the major rice diseases affecting rice output in Indonesia causing a 90% reduction in yield. This study aimed to determine the distribution of Pyricularia oryzae virulence pathotypes (races) in several South Sulawesi regencies. The P. oryzae races were determined in the Green House using 7 rice differential varieties: Asahan, Cisokan, IR 64, Krueng Aceh, Cisadane, Cisanggarung, and Kencana Bali. A total of 72 P. oryzae isolates from Maros, Gowa, Bone, and Pinrang Regencies were tested. The IRRI evaluation standard assessed the disease intensity seven days after inoculation. There were a total of 24 race compositions of P. oryzae were discovered based on the reactions of different rice varieties. However, only two races were dominant. Race 001 was dominant among Pinrang and Maros isolates, whereas race 020 was dominant in Bone and Gowa. Based on this result, monitoring of disease severity and the dynamics of P. oryzae population race structures is important for the breeding and rational distribution of resistant rice varieties.

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Elucidating genetic diversity and population structure of Pyricularia oryzae isolates causing wheat blast in Bangladesh

2022

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Morphological and molecular characterization of Pyricularia oryzae isolates causing wheat blast in Bangladesh

Cited by 3 publications

References 26 publications

Morphological and molecular identification of Pyricularia oryzae causing blast disease on rice (Oryza sativa)

Morphological and molecular identification of Pyricularia oryzae causing blast disease on rice (Oryza sativa)

Population structures of Pyricularia oryzae Cavara in South Sulawesi

Elucidating genetic diversity and population structure of Pyricularia oryzae isolates causing wheat blast in Bangladesh

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