2015
DOI: 10.1007/s10658-015-0674-z
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Morphological and molecular charaterisation of Pratylenchus parazeae n. sp. (Nematoda: Pratylenchidae) parasitizing sugarcane in China

Abstract: A new root-lesion nematode was isolated from sugarcane (Saccharum sinensis Roxb.) in Guangxi Zhuang Autonomous Region, China. Morphology and molecular analyses of rRNA small subunit (SSU), D2D3 expansion domains of large subunit (LSU D2D3) and internal transcribed spacer (ITS) confirmed that this nematode is different from other previously described root-lesion nematodes. Herein it is described, illustrated and named as Pratylenchus parazeae n. sp. This new species is characterised by plain and smooth En face,… Show more

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Cited by 32 publications
(15 citation statements)
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“…However, accurate diagnose of Pratylenchus spp. needs to be supported by means of morphological and molecular techniques (Subbotin et al 2008;De Luca et al 2011;Wang et al 2015;Nguyen et al 2017;Divsalar et al 2018). Among the DNA barcoding markers, the 28S rDNA (Carta et al 2001;Al-Banna et al 2004;De Luca et al 2004, 2011Subbotin et al 2005Subbotin et al , 2008 and COI of mtDNA (Singh et al 2018) have been used for Pratylenchus spp.…”
Section: Introductionmentioning
confidence: 99%
“…However, accurate diagnose of Pratylenchus spp. needs to be supported by means of morphological and molecular techniques (Subbotin et al 2008;De Luca et al 2011;Wang et al 2015;Nguyen et al 2017;Divsalar et al 2018). Among the DNA barcoding markers, the 28S rDNA (Carta et al 2001;Al-Banna et al 2004;De Luca et al 2004, 2011Subbotin et al 2005Subbotin et al , 2008 and COI of mtDNA (Singh et al 2018) have been used for Pratylenchus spp.…”
Section: Introductionmentioning
confidence: 99%
“…a. the FAM signal showing specificity for P. neglectus, Gsy24S, Sd12922, Sdwg and Xz9 represent four populations of P. neglectus; nontarget nematodes including P. thornei, P. coffeae, P. brachyurus, P. loosi, P. parazeae, P. scribneri, P. vulnus, P. zeae, Meloidogyne incognita, M. enterolobii, Heterodera fengi, Aphelenchoides besseyi and Caenorhabditis elegans; b. the ROX signal showing specificity for P. thornei. Gsy24L and Xjdm represent two populations of P. thornei; non-target nematodes including P. neglectus, P. coffeae, P. brachyurus, P. loosi, P. parazeae, P. scribneri, P. vulnus, P. zeae, M. incognita, M. enterolobii, H. fengi, A. besseyi and C. elegans Pratylenchus species (De Luca et al 2011;Wang et al 2015), making it difficult to design species-specific primers. Intraspecific variations in the ITS-rDNA of P. neglectus and P. thornei could reach 7% and 6% respectively (De Luca et al 2011).…”
Section: Discussionmentioning
confidence: 99%
“…Some of these have been used separately or in combination with traditional methods to accurately characterize and to fingerprint Pratylenchus species (Waeyenberge et al, 2000;Waeyenberge et al, 2009;De Luca et al, 2011). The use of the former approach has been very successful because of the inter-genetic variation in the ITS1 and ITS2 sequences of different species, notwithstanding any intra-genetic variations in individual nematodes of the same species (Duncan et al, 1999;Waeyenberge et al, 2000;Waeyenberge et al, 2009;Wang et al, 2015). During the analyses of our data, it was surprising to note that although there…”
Section: Discussionmentioning
confidence: 99%