Morphological and molecular identification of Pseudoplagiostoma castaneae sp. nov. (Pseudoplagiostomataceae, Diaporthales) in Shandong Province, China

Taxonomy, Phylogeny, Divergence Time Estimation, and Biogeography of the Family Pseudoplagiostomataceae (Ascomycota, Diaporthales)

“…Most species of Pseudoplagiostomataceae were isolated from Eucalyptus ( Myrtaceae ) ( Pseudoplagiostoma corymbiae , P. corymbiicola , P. eucalypti , P. oldii , and P. variabile ), especially P. eucalypti with more than 40 strains [ 1 , 4 , 5 , 10 ]. Recently, other hosts were reported, including Anacardiaceae ( P. mangiferae and P. myracrodruonis ), Dipterocarpaceae ( P. dipterocarpi and P. dipterocarpicola ), Fagaceae ( P. castaneae ) [ 2 , 3 , 7 , 8 , 9 ]. This study puts more families in the host list, and they are Cyatheaceae ( P. alsophilae ), Gramineae ( P. bambusae ), and Lauraceae ( P. machili ).…”

“…Novel sequences obtained in this study and related sets of sequences from Mu et al [ 2 ] were aligned with MAFFT v. 7 and corrected manually using MEGA 7 [ 33 ]. Multi-locus phylogenetic analyses were based on the algorithms maximum likelihood (ML) and Bayesian inference (BI) methods.…”

“…The sexual morph is characterized by immersed, beaked, ostiole ascomata, unitunicate asci, a non-amyloid subapical ring, hyaline ascospores that are 1-septate near the middle or aseptate, with terminal, elongated hyaline appendages. The asexual morph is characterized by superficial and immersed conidiomata with masses of apically proliferous conidiogenous cells and hyaline, ellipsoidal conidia, but with no conidiophores [ 1 , 2 , 3 ].…”

“…Pseudoplagiostoma species were mainly reported as endophytes, plant pathogens, or saprobes in various regions, viz. Asia, North America, Oceania, and South America [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. As a type species, P. eucalypti was reported with more than 40 strains in the whole world (NCBI Nucleotide database, , accessed on 6 December 2022).…”

“…Recently, Pseudoplagiostoma as an endophyte from Castanea mollissima and Dipterocarpus sp. was introduced by Mu et al [ 2 ] and Tang et al [ 9 ]. This was the first time that Pseudoplagiostoma species had been found on the host of Castanea and Dipterocarpus .…”