Morphological and Molecular Screening of Soybean Genotypes against Yellow Mosaic Virus Disease

Abstract

Background: The growth and productivity of soybean are adversely affected by an array of biotic factors. Viruses are one of them as they cause great loss to the yield of soybean in India. The present study was conducted with an objective to identify yellow mosaic virus (YMV) resistant genotypes among the selected set of 53 soybean genotypes. Methods: The field screening was performed to identify YMV resistant genotypes. The field data was compared with molecular data recorded on the basis of gene specific SSR molecular markers. Result: During field study, 11 genotypes were found to be highly resistant, 26 resistant, 6 moderately resistant, 4 moderately susceptible, 3 susceptible, while three genotypes namely: JS335, JS 97-52 and RVS 2001-4 were found to be highly susceptible. In molecular analysis three genotypes viz.,: JS 20-29, JS 20-69 and JS 20-98 were found to be resistant against YMV. Among the polymorphic SSR markers the highest genetic diversity (0.4785) was observed with Satt554 while lowest genetic diversity (0.037) was observed with Satt308. Similarly polymorphism information content (PIC) was highest (0.364) in Satt554 and lowest (0.0363) in Satt308 among all polymorphic markers used for screening against YMV. The resistant genotypes identified in this study may be used as donor of resistance gene against YMV to develop improved genotypes which would stand as barrier against spread of the disease to newer areas and thus it can boost production and productivity of soybean in the country.

In vitro Selection for Resistance against Charcoal Rot Disease of Soybean [Glycine max (L.) Merrill] Caused by Macrophomina phaseolina (Tassi) Goid

“…DNA extraction of both mother genotypes viz., JS335 and JS95-60 as well as selected putative charcoal rot resistant plants was performed according to the protocol adopted (Mishra et al 2020). PCR amplifications were done with five random decamer primers namely OPC-20, OPA-11, OPB-07, OPC-03 and OPE-11.…”

“…Soybean [Glycine max (L.) Merrill] is an economically important dicot legume in the world's oilseed cultivation scenario. Continuous cultivation and simultaneous increase in area of soybean has led to increase in disease (Mishra et al 2020) that ultimately resulted in yield losses. Among biotic stresses, charcoal rot caused by soil borne polyphagous fungus Macrophomina phaseolina (Tassi) Goid is a major reason of yield loss in soybean (Yang and Navi, 2005).…”

Morphological and Physiological Performance of Indian Soybean [Glycine max (L.) Merrill] Genotypes in Respect to Drought

“…Drought stress escorts to interruption in stomatal mechanism, gas exchange, metabolism activities, cell structure, ultimately enzyme catalyzed reactions and expression of diverse genes (Paul et al 2011). Genetic improvement of stress tolerance in crop plants necessitates recognition of pertinent morphophysiological parameters to be employed as selection criteria (Kumar et al 2014;Sepanlo et al 2014;Kachre et al 2019;Mishra et al 2020;Upadhyay et al 2020a;2020b).…”

Screening of Pearl Millet [Pennisetum glaucum (L.) R. Br.] Germplam Lines for Drought Tolerance Based on Morpho-physiological Traits and SSR Markers

“…In the case of drought tolerance, availability of markers tightly linked to tolerant genes will help in identifying plants carrying these genes. Microsatellite markers are adjudged as the most effective and reliable DNA markers for such studies due to their abundance in the genome, multi allelism, genome specificity and even distribution, multi-allelism easy detection, high-throughput, highly reproducible and codominantly inherited behaviour [12,13,14,15,16,17,18,19,20,21,22]. Therefore, the use of SSR markers is a precious approach for the diversity analysis among pearl millet genotypes.…”