Morphological and physicochemical aspects of microbial lipase obtained from novel agroindustrial waste encapsulated in a sol–gel matrix

Zubiolo, Claudia; Santos, Rafaela Cristiane Andrade; Figueiredo, Renan Tavares; Soares, Cleide Mara Faria; Santana, Luciana Cristina Lins de Aquino

doi:10.1007/s10973-015-4494-3

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…More details on microbial lipases can be noted from recently reported review papers covering the overview on microbial lipases, microbial alkaline lipases, and purification of microbial lipases . Further, it has been recently revealed that microbial lipases can also be obtained through fermentation of agricultural waste and dairy waste contributing to the environmental protection and they may be exploited for future bio‐catalytic applications.…”

Section: Sources Of Lipasesmentioning

confidence: 99%

Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

301

148

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Lipases are the industrially important biocatalysts, which are envisioned to have tremendous applications in the manufacture of a wide range of products. Their unique properties such as better stability, selectivity and substrate specificity position them as the most expansively used industrial enzymes. The research on production and applications of lipases is ever growing and there exists a need to have a latest review on the research findings of lipases. The present review aims at giving the latest and broadest overall picture of research and development on lipases by including the current studies and progressions not only in the diverse industrial application fields of lipases, but also with regard to its structure, classification and sources. Also, a special emphasis has been made on the aspects such as process optimization, modeling, and design that are very critical for further scale-up and industrial implementation. The detailed tabulations provided in each section, which are prepared by the exhaustive review of current literature covering the various aspects of lipase including its production and applications along with example case studies, will serve as the comprehensive source of current advancements in lipase research. This review will be very useful for the researchers from both industry as well as academia in promoting lipolysis as the most promising approaches to intensified, greener and sustainable processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:5-28, 2018.

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Section: Sources Of Lipasesmentioning

confidence: 99%

Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

301

148

View full text Add to dashboard Cite

show abstract

“…Since 1996, the sol-gel production method has been gradually improved and has formed second-generation sol-gel matrixes with the use of a variety of additives [16]. The patent PI0306829-3 introduced a simple and general method to synthesize sol-gel matrices and was used extensively in research [17]. Ranyere et al [18] have reported the affection of polyethylene glycol (PEG, MW 1500) in various concentrations as additives during immobilization.…”

mentioning

confidence: 99%

Immobilized Aspergillus niger Lipase with SiO2 Nanoparticles in Sol-Gel Materials

Huang

et al. 2016

Catalysts

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Lipase from Aspergillus niger was "doubly immobilized" with SiO 2 nanoparticles in sol-gel powders prepared via the base-catalyzed polymerization of tetramethoxysilane (TMOS) and methyltreimethoxysilane (MTMS). The hydrolytic activity of the immobilized lipase was measured using the p-nitrophenyl palmitate hydrolysis method. The results showed that the optimum preparation conditions for the gels were made using a MTMS/TMOS molar ratio of 5, 60 mg of SiO 2 nanoparticles, a water/silane molar ratio of 12, 120 mg of enzyme supply, and 120 µL of PEG400. Under the optimal conditions, the immobilized lipase retained 92% of the loading protein and 94% of the total enzyme activity. Characteristic tests indicated that the immobilized lipase exhibited much higher thermal and pH stability than its free form, which shows great potential for industrial applications.

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Sol–gel encapsulation of pullulanase in the presence of hybrid magnetic (Fe3O4–chitosan) nanoparticles improves thermal and operational stability

Long

Zhan

et al. 2017

Bioprocess Biosyst Eng

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Pullulanase was sol-gel encapsulated in the presence of magnetic chitosan/FeO nanoparticles. The resulting immobilized pullulanase was characterized by scanning electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy and thermogravimetric analysis. The results showed that the addition of pullulanase created a more regular surface on the sol-gel matrix and an enhanced magnetic response to an applied magnetic field. The maximal activity retention (83.9%) and specific activity (291.7 U/mg) of the immobilized pullulanase were observed under optimized conditions including an octyltriethoxysilane:tetraethoxysilane (OTES:TEOS) ratio of 1:2 and enzyme concentration of 0.484 mg/mL sol. The immobilized enzyme exhibited good thermal stability. When the temperature was above 60 °C, the immobilized pullulanase showed significantly higher activity than the free enzyme (p < 0.01); enzyme immobilized by simple sol-gel encapsulation and co-immobilized by crosslinking-encapsulation retained 52 and 69% of their initial activity after 5 h at 62 °C, respectively, compared to 11% for the free enzyme. Moreover, the stability of the pullulanase was improved by crosslinking-encapsulation, as the enzyme retained more than 85 and 81% of its original activity after 5 and 6 consecutive reuses, respectively, compared to 80 and 72% of its original activity for simple sol-gel encapsulated enzymes. This indicated the leakage of enzyme molecules through the pores of the gel was substantially abated by cross-linking. Such immobilized pullulanase provides high stability and ease of enzyme recovery, characteristics that are advantageous for applications in the food industry that involve continuous starch processing.

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Morphological and physicochemical aspects of microbial lipase obtained from novel agroindustrial waste encapsulated in a sol–gel matrix

Cited by 4 publications

References 32 publications

Recent advances on sources and industrial applications of lipases

Recent advances on sources and industrial applications of lipases

Immobilized Aspergillus niger Lipase with SiO2 Nanoparticles in Sol-Gel Materials

Sol–gel encapsulation of pullulanase in the presence of hybrid magnetic (Fe3O4–chitosan) nanoparticles improves thermal and operational stability

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