Morphological and physiological changes in bacterial cells treated with acrylamide

Decolorization of an industrial effluent by free and immobilized cells of Stenotrophomonas maltophilia AAP56. Implementation of efficient down flow column reactor

“…However, the exposure of bacteria to polyacrylamide monomer during the immobilization affected seriously some of metabolic and physiological activities of the bacteria. Starostina et al (1983) reported that the bacteria underwent most changes in course of immobilization in the polyacrylamide gel: blockage of cell division, elongation of cells, inhibition of nucleic acid synthesis (mostly DNA), decrease in osmotic stability and ultrastructural alterations of the outer membrane. The decrease of the ability of S. maltophilia AAP56 to decolorize the effluent can be related to these metabolic and morphological alterations.…”

Poly-N-vinylcaprolactam gel: A novel matrix for entrapment of microorganisms

“…However, the number of polymers used to immobilize viable cells is limited by the toxic influences of monomers, hardening or crosslinkable agents during polymerization and immobilization processes. For instance, during the acrylamide polymerization monomers can considerably reduce cell viability and enzyme activity [Siess and Devies, 1981;Starostina et al, 1983]. To diminish this effect, short-time polymerization at low temperatures (0-4oc) or use of prepolymerized linear compounds were proposed [Pines and Freeman, 1982].…”

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

“…Brought to you by | provisional account Unauthenticated | 129.173.72.87 Download Date | 7/10/14 9:40 AM level of the "immobilisation shock" of polyaciylamide entrapped cells depended to a large extent on the initial physiological state of the population (Starostina et al, 1983;Lusta et al, 1990). Bacterial clones of E. coli and Pseudomonas putida with increased resistance to polyacrylamide immobilisation and acrylamide monomer action have been isolated (Starostina et al, 1986;Lusta et al, 1990).…”

“…Bacterial clones of E. coli and Pseudomonas putida with increased resistance to polyacrylamide immobilisation and acrylamide monomer action have been isolated (Starostina et al, 1986;Lusta et al, 1990). By carefully controlling the polymerisation time and temperature, 100% viability of the Arthrobacter globiformis cells could be retained (Koshcheenko et al, 1983;Skryabin and Koshcheenko, 1987).…”