Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro

Borkowska, B.

doi:10.1016/s0304-4238(00)00230-2

Cited by 56 publications

(25 citation statements)

References 13 publications

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“…It suggests that good root growth of the plantlets would support shoot growth during acclimatization period. Similar results were reported by Borkowska (2001) in micropropagated strawberry rooted ex vitro. Roots have a physical role as an anchor to support plantlets to stand upright and a physiological role to uptake water and nutrients for plant growth and development.…”

Section: Shoot Growthsupporting

confidence: 90%

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EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Sumaryono¹,

Riyadi²

2011

Indones. J. Agric. Sci.

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Plantlets of oil palm (Elaeis guineensis Jacq.) derived from somatic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with auxin during ex vitro acclimatization period to simplify the procedure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene-acetic acid (NAA) at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm) for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequency to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indicated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diameter by 30% compared to control treatment, but did not influence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plantlets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.

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Section: Shoot Growthsupporting

confidence: 90%

“…Ex vitro rooting is conducted to simplify tissue culture protocols and to reduce the production cost (Meier-Dinkel et al 1993;Borkowska 2001;Hazarika 2003;Martin 2003;Shekafandeh 2007). Furthermore, ex vitro rooting and acclimatization phases could be done at the same time, hence it is more efficient.…”

Section: Introductionmentioning

confidence: 99%

EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Sumaryono¹,

Riyadi²

2011

Indones. J. Agric. Sci.

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“…These characteristics were also observed in strawberry (Fragaria x Ananassa Duch.) (Borkowska 2001) and in Siratia grosvenorii (Yan et al 2010). However, this is not common to all plant species, as observed in Dickia distachya (Pompelli & Gerra 2005) …”

Section: Concentration (µM)mentioning

confidence: 90%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.

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“…From Borkowska [2000] studies resulted, that the rooting of strawberry in vitro plants should be done ex vitro.…”

Section: Rootingmentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

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Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro

Cited by 56 publications

References 13 publications

EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

In vitro REGENERATION OF Fragraria PLANTS

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