Morphological and physiological variation among different isolates of Alternaria spp. from Rapeseed-Mustard

Monowara, Rufaida; Tonu, Nazmoon Naher; Begum, Fatema; Karim, Md. Masud; Sultana, Nazneen

doi:10.22161/ijeab/2.5.21

Cited by 2 publications

(3 citation statements)

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“…showed variation in mycelial growth, colony color, shape, textures, subsurface color, zonation conidia production and sporulation time. Almost same observation was reported by Rufaida Monowara et al (2017) where they studied with 10 Alternaria isolates, and reported that all 10 isolates showed significant variations in respect of their cultural and morphological characteristics on different artificial culture media. The results are also similar with Yadav et al (2016), they were identified morphological and cultural characters of A. brassicae isolates from four different locations, colonies of all the isolates were circular in shape.…”

Section: Discussionsupporting

confidence: 80%

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Isolation and Morpho-Molecular Characterization of Alternaria spp. Causing Grey Leaf Blight of Mustard in Different Regions of Bangladesh

Kabir,

Fakir,

Millat

et al. 2023

Preprint

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Mustard (Brassica spp.) is the most important oil seed crop in Bangladesh and the major constraint is grey blight disease which caused significant yield losses. The prime aim of the study was to characterize the Alternaria species on the basis of morpho-molecular variation responsible for grey blight disease of mustard in different regions of Bangladesh. The experiment was conducted in Molecular Biology and Plant Virology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka and Plant Pathology Laboratory, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur. Diseased mustard leaves displaying characteristic symptoms were collected from eight distinct mustard-growing districts in Bangladesh, namely Jashore, Khulna, Satkhira, Dhaka, Manikganj, Tangail, Sirajganj, and Dinajpur. Fungal DNA was extracted from pure cultures of Alternaria, and PCR amplification was conducted using ITS primers designed to yield 700 bp fragments. Morphological characterization revealed considerable variations among Alternaria species, including differences in radial mycelial growth, colony color, shape, texture, subsurface color, zonation, conidia production, and sporulation time. Pathogenicity tests confirmed the pathogenic nature of all Alternaria isolates. Molecular analysis using ITS regions demonstrated a 99% similarity of the nucleotide sequences with existing sequences in the NCBI database, closely related to Alternaria brassicae, Alternaria brassicicola, and Alternaria alternata. Three species of Alternaria (A. brassicae, A. brassicicola, and A. alternata) were identified from grey blight-infected mustard leaves. Additionally, variations were observed in the number of conidia, longitudinal septation, transverse septation, conidial length, and breadth among the different isolates. The study highlighted the close genetic relationship among the Alternaria isolates and their association with grey blight disease in mustard. These findings contribute valuable insights into the genetic diversity and pathogenicity of Alternaria species in Bangladesh's mustard-growing regions. Such knowledge can be instrumental in developing effective disease management strategies to mitigate yield losses in this crucial oilseed crop.

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Section: Discussionsupporting

confidence: 80%

“…Variation of conidial breath of Alternaria spp. was observed that was 5.57 to 7.15 µm but no statistically significant data was found among the isolates Monowara et al (2017). reported that variations were observed in accordance with length, breadth and beck on different isolates of Alternaria spp.…”

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confidence: 85%

Isolation and Morpho-Molecular Characterization of Alternaria spp. Causing Grey Leaf Blight of Mustard in Different Regions of Bangladesh

Kabir,

Fakir,

Millat

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The plates were sealed and incubated at 27±2EC for seven days. Six replicates were performed for each isolate and culture medium in a complete randomization experimental design 17 . Fungal colony diameter measurements were performed daily using a graduated ruler.…”

Section: Identification Of Fungal Isolatesmentioning

confidence: 99%

Morphometric Analysis of Isolated Conidia of Various Species of Colletotrichum sp. from Avocado and Mango in Côte d'Ivoire

Kouadia¹,

Tienebo²,

Kouadio³

et al. 2023

Plant Pathology J.

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Background and Objective: Fruits are important sources of income for farmers. However, they are subject to many diseases caused mainly by fungi, of which the genus Colletotrichum. The genus Colletotrichum is an important and frequent causal agent of post-harvest infections of fruits. Although this genus can infect a wide range of hosts, one or more species of Colletotrichum may be found on the same host. Based on morphological characteristics, this study aimed to differentiate 49 isolates of Colletotrichum sp., obtained from avocados and mangoes from the main production areas in Côte d'Ivoire. Materials and Methods: In this study, the growth rate of Colletotrichum isolates was evaluated on three culture media (potato dextrose agar (PDA), malt agar and corn meal agar (CMA) media), as well as their sporulation. The size of the spores was measured and their shapes were noted. Descending hierarchical classification (DHC) was used to group the isolates by size. A comparison of isolates obtained from the two fruits was performed by Principal Component Analysis (PCA) based on all morphological characteristics studied. Results: The study revealed a very high morphological variability in the conidia of Colletotrichum isolates. DHC grouped avocado isolates into 11 classes and mango isolates into four classes. Spore shape analysis revealed a majority of spores with either both ends rounded or one end rounded and the other sharp. Growth and sporulation of the isolates were best on the malt medium. PCA revealed that mango isolates were distinct from avocado isolates. Conclusion: This study showed that most isolates had cylindrical or ellipsoidal spores with rounded or one sharp end, with better growth and sporulation on the malt medium. However, these isolates were distinct and reported to have characteristics of Colletotrichum gloeosporioides and Colletotrichum acutatum.

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Morphological and physiological variation among different isolates of Alternaria spp. from Rapeseed-Mustard

Abstract: Abstract-

Cited by 2 publications

References 10 publications

Isolation and Morpho-Molecular Characterization of Alternaria spp. Causing Grey Leaf Blight of Mustard in Different Regions of Bangladesh

Isolation and Morpho-Molecular Characterization of Alternaria spp. Causing Grey Leaf Blight of Mustard in Different Regions of Bangladesh

Morphometric Analysis of Isolated Conidia of Various Species of Colletotrichum sp. from Avocado and Mango in Côte d'Ivoire

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