Morphological and Surface Properties of Electrospun Chitosan Nanofibers

Desai, Keyur; Kit, K. M.; Li, Jiajie; Zivanovic, Svetlana

doi:10.1021/bm701017z

Cited by 241 publications

(154 citation statements)

References 27 publications

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“…25 Briefly, the electrospinning setup used in this study consisted of three major components: a power supply using direct current that could generate a voltage of up to 30 kV; a 3 mL syringe with a metallic needle with a 0.65 mM inner diameter that could control the flow rate of a scientific pump (model 780/00; KD Scientific, Holliston, MA, USA); and a collector made from aluminum foil for fiber collection. Chitosan was dissolved in trifluoroacetic acid/dichloromethane (70/30 vol %) at a concentration of 50 mg/mL, and the electrospinning solutions were stirred for 24 hours into well-mixed homogeneous solutions.…”

Section: Electrospinning Of Chitosanmentioning

confidence: 99%

Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Liao²,

Lin³

et al. 2014

IJN

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Osteoblast maturation plays a key role in regulating osteogenesis. Electrospun nanofibrous products were reported to possess a high surface area and porosity. In this study, we developed chitosan nanofibers and examined the effects of nanofibrous scaffolds on osteoblast maturation and the possible mechanisms. Macro- and micro observations of the chitosan nanofibers revealed that these nanoproducts had a flat surface and well-distributed fibers with nanoscale diameters. Mouse osteoblasts were able to attach onto the chitosan nanofiber scaffolds, and the scaffolds degraded in a time-dependent manner. Analysis by scanning electron microscopy further showed mouse osteoblasts adhered onto the scaffolds along the nanofibers, and cell–cell communication was also detected. Mouse osteoblasts grew much better on chitosan nanofiber scaffolds than on chitosan films. In addition, human osteoblasts were able to adhere and grow on the chitosan nanofiber scaffolds. Interestingly, culturing human osteoblasts on chitosan nanofiber scaffolds time-dependently increased DNA replication and cell proliferation. In parallel, administration of human osteoblasts onto chitosan nanofibers significantly induced osteopontin, osteocalcin, and alkaline phosphatase (ALP) messenger (m)RNA expression. As to the mechanism, chitosan nanofibers triggered runt-related transcription factor 2 mRNA and protein syntheses. Consequently, results of ALP-, alizarin red-, and von Kossa-staining analyses showed that chitosan nanofibers improved osteoblast mineralization. Taken together, results of this study demonstrate that chitosan nanofibers can stimulate osteoblast proliferation and maturation via runt-related transcription factor 2-mediated regulation of osteoblast-associated osteopontin, osteocalcin, and ALP gene expression.

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Section: Electrospinning Of Chitosanmentioning

confidence: 99%

Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Liao²,

Lin³

et al. 2014

IJN

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“…The filter media showed high efficiencies to air and water filtration, which correlated with the fibrous media size and shape. Similar nonwoven fiber mats with fiber diameter as low as 80±35 nm for air and water filtration were also made by electrospinning high molecular weight chitosan/PEO (95:5) blends [106]. An affinity membrane made from electrospun hybrid chitosan/nylon-6 nanofibers by nucleophilic reaction of the chitosan's hydroxyl and amidocyanogen with the triazinyl chloride of Cibacron Blue F3GA (CB) ligand was used to study the purification of papain [107].…”

Section: Filtrationmentioning

confidence: 99%

Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning

Sun¹,

Li²

2011

Express Polym. Lett.

218

101

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Chitosan is soluble in most acids. The protonation of the amino groups on the chitosan backbone inhibits the electrospinnability of pure chitosan. Recently, electrospinning of nanofibers based on chitosan has been widely researched and numerous nanofibers containing chitosan have been prepared by decreasing the number of the free amino groups of chitosan as the nanofibiers have enormous possibilities for better utilization in various areas. This article reviews the preparations and properties of the nanofibers which were electrospun from pure chitosan, blends of chitosan and synthetic polymers, blends of chitosan and protein, chitosan derivatives, as well as blends of chitosan and inorganic nanoparticles, respectively. The applications of the nanofibers containing chitosan such as enzyme immobilization, filtration, wound dressing, tissue engineering, drug delivery and catalysis are also summarized in detail

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“…Chitosan [27,28] is a polysaccharide obtained by deacetylation of natural chitin, which is one of the important natural polymers found in the shells of crustaceans and the cell walls of many fungi. Due to its oxygen linkages to nitrogen from the NH 2 groups and OH moieties present in the polysaccharide chains, it can bind metal ions forming macromolecular metal complexes [29].…”

Section: Introductionmentioning

confidence: 99%