Morphological Assessment of Wound Healing After Cryodestruction of Skin Using an Aqueous Colloidal Solution of C60 Fullerenes

Abstract

The aim is to assess the morphological state of a cold skin wound in an experiment, using an aqueous colloidal solution of C60 fullerenes. Materials and methods: An experimental study was conducted in 30 six-month-old male rats divided into three groups, 10 individuals in each group: group I included rats that did not undergo any manipulations; group II was represented by rats which underwent a cold wound simulation on the lateral thigh surface; group III was represented by rats who had a simulated cold wound followed by introduction of an aqueous colloidal solution of C60 fullerenes at a dose of 0.1 mg/kg for 5 days into the abdominal cavity, starting from the cryodamage day. Animals of all groups were removed from the experiment on days 7, 14 and 21. The material for the morphological study was skin with underlying soft tissues. The resulting sample was studied, using histological, histochemical, morphometric and statistical research methods. Results: C60 fullerenes stimulate proliferative activity in the epidermis, located in the wound marginal regions or covering the regenerate surface, which leads to an increase in epithelial layer thickness by 2.6, 2.6, 2.5 times on 7, 14 and 21 days; promotes faster cleansing of the wound from necrotic tissue, reducing the thickness of the zone of primary necrosis on days 7, 14 and 21 by 1.3 times by increasing the demarcation zone on days 7, 14 and 21 by 1.4, 1.4 and 2, 2 times; reduces the thickness of the secondary necrosis area on 7, 14 and 21 days by 1.4, 1.5 and 1.3 times; accelerates filling of the wound defect with granulation tissue, the layer thickness of which increases by 2.3, 2.2 and 1.4 times on the 7th, 14th and 21st days, respectively; reduces the severity of hemodynamic disorders and cell infiltration in the wound defect adjacent tissues of the dermis, hypodermis with the underlying muscle tissue. Conclusions: A comprehensive morphological study of the experimental material indicates that the injection into the abdominal cavity of an aqueous colloidal solution of C60 fullerenes activates reparative processes in the skin cold wounds, which go through all the classical stages.

A comprehensive review on the applications of carbon-based nanostructures in wound healing: from antibacterial aspects to cell growth stimulation

“…To treat cold skin wounds, Vlasov et al proved that the abdominal cavity injection of a C60 fullerene colloidal solution stimulated multiplication in the epidermis of the areas surrounding the wound, which accelerated the wound cleansing from necrotic tissue, increased the thickness of the epithelial layer and decreased the thickness of the primary and secondary necrosis area. 217 Activated carbon (AC), as another carbon nanostructure, is biocompatible, possesses a large active site and is widely used in biomedical applications such as wound healing because of its high adsorption property. One important factor in patients is their physical and mental well-being during the wound healing process, especially in chronic wounds, where malodor control is necessary.…”

“…To treat cold skin wounds, Vlasov et al proved that the abdominal cavity injection of a C60 fullerene colloidal solution stimulated multiplication in the epidermis of the areas surrounding the wound, which accelerated the wound cleansing from necrotic tissue, increased the thickness of the epithelial layer and decreased the thickness of the primary and secondary necrosis area. 217…”

Morphological Efficacy Evaluation of Gel With Carbon Dioxide Extract of Hops in Case of Complicated Wound Infection Acne Vulgaris

“…The data obtained indicate the organotypic healing of the wound defect. Organotypic regeneration, as is known, is one of regeneration types when an organ defect is replaced by a regenerate corresponding to the organ's structure [13,14].…”